We narrowed to 9 results for: PID

1. AAV Production in HEK293 Cells

   Type

   Protocol

   ...565-0010 (or for larger volumes 1000 mL 0.45 µm Rapid-Flow PES Filtration Unit, Nalgene 167-0045) Pro-...-Tip The pH of this solution will drift pretty rapidly upon addition of acid or base. Add only a few drops...

2. Fluorescence Titering Assay

   Type

   Protocol

   ...associated with freeze-thaw. If using frozen virus, rapidly thaw the lentiviral aliquot on ice prior to use...

3. General Transfection

   Type

   Protocol

   ...-Tip The pH of this solution will drift pretty rapidly upon addition of acid or base. Add only a few drops...

4. Lentivirus Production

   Type

   Protocol

   ...first. Pro-Tip The pH of this solution will drift rapidly upon addition of acid or base. Add only a few drops...

5. Transfection for Recombinant Antibodies

   Type

   Protocol

   ...have dissolved. Note: The powder should dissolve rapidly (with 20 min) but check for the presence of particles...

6. AAV Purification by Iodixanol Gradient Ultracentrifugation

   Type

   Protocol

   ... to collect the next fraction. The flow will be rapid at first and will progressively slow down. Option...

7. What is Polymerase Chain Reaction (PCR)

   Type

   Protocol

   ...elongate the new target DNA strand accurately and rapidly. Repeat steps 2-4 25-30 times. Final Extension ...

8. Virus Protocol - Generating Stable Cell Lines

   Type

   Protocol

   ...especially if that transgene is toxic. Eventually, the rapidly growing low-level transgene expressors may take...

9. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

   Type

   Protocol

   ...430281 0.45 µm PES complete filtration unit, 500 mL, rapid-flow, VWR 73520-984 Syringes, 30 mL, VWR BD302832...