Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 181 results for: PID

Showing: 1 - 20 of 181 results

  1. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

    Type
    Blog Post
    Published
    Nov. 20, 2018, 8:17 p.m.
    ...solution is the use of unique persistent identifiers (PIDs), a long-lasting way to identify and reference documents...Perhaps the most commonly known form of a unique PID is the Digital Object Identifier (DOI). Nearly all... to be persistent and exist online indefinitely. PIDs historically referred to objects that exist online...numbers associated with them, which serve as unique PIDs within the Addgene catalog. These IDs allow you ...now recommend or require authors to use specific PID formats. For example, several journals like eLife...which are supported by SciCrunch. Other well known PID formats are resolvable compact identifiers, which...compact identifier for every plasmid. In case these PID formats are new to you, we’ll go through examples...

  2. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...subtracted from Ramanathan et al.’s RaPID datasets. Applications of RaPID RaPID sounds like a nifty technique...created the RNA-protein interaction detection (RaPID) method. RaPID borrows the E. coli biotin ligase BirA* ...organisms. Detecting RNA-protein interactions with RaPID RaPID is similar to the BioID platform for identifying...signaling. Limitations of RaPID Like other BirA*-based proximity labeling methods, RaPID cannot distinguish ...native regulatory elements. Controls for RaPID experiments RaPID negative controls include both scrambled... of how RaPID can be used to study RNA-protein interactions. Do you have a question that RaPID could help...identifying protein-protein interactions, but RaPID identifies RNA-protein interactions. Both methods rely on...

  3. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    Published
    Dec. 2, 2019, 2 p.m.
    ...APEX: engineered ascorbate peroxidase that can rapidly biotin label proteins A year after BioID became...  Find the APEX2 plasmids! TurboID and minturbo: Rapid and non-toxic proximity labeling  TurboID and miniTurbo...hours to label proteins and APEX2, although it can rapidly label proteins, requires H2O2 which can be toxic...more information, read the Split-BioID blog post.  RaPID: RNA-protein interaction detection So far, we’ve...that can regulate multiple biological functions. RapID (RNA-protein interaction detection) uses proximity...interactions (Ramanathan et al., 2018). To develop RapID, scientists engineered a mutant BirA* from B. subtilis... compared to the standard E. coli BirA* mutant. RapID consists of this mutant BirA* and an RNA component...

  4. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ... agarose addition, we found it was possible to rapidly punch out cell clones with sterile truncated pipet...from a 10 cm dish in 15- 20 minutes- a far more rapid pace than using cloning rings. A picture of a 60...only warm but not hot- and still liquid- it is rapidly poured onto the middle of the plate, where it immediately...and partially dislodged from the agarose plug by rapid pipetting, as shown in the YouTube video. Before... appear to be yielding well-spaced clones. For rapidly growing cells we sometimes use TPP peelaway T-75...drop of serum to each well in the 48-well plate; rapidly growing cells such as HEK and CHO do not seem to...technique should be useful to scientists who wish to rapidly isolate individual cell clones with different expression...

  5. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...CRISPR/Cas based COVID-19 testing methods.   Rapid detection of 2019 novel coronavirus SARS-CoV-2 using...incorporated into a lateral-flow readout slip that allows rapid detection.  Highlights: Cas13a is not catalytically...All-in-one dual CRISPR/Cas12a (AIOD-CRISPR) assay – for rapid, ultrasensitive and visual detection of novel coronavirus...operated, made accessible by the DIY-Bio movement. Rapid, field-deployable nucleobase detection and identification... D., Sharma, N., Kumar, M., … Maiti, S. (2020). Rapid, field-deployable nucleobase detection and identification..., & Alejandra, G. C. (2020). An ultrasensitive, rapid, and portable coronavirus SARS-CoV-2 sequence detection...

  6. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ... read our secondary nanobodies blog post.  A rapid selection platform for generating sybodies against...fewer hydrophilic surfaces, require detergents or lipids to remain folded, and are often less abundant than...developed a robust in-vitro protocol that enables rapid selection of synthetic nanobodies, called sybodies...for further characterization and analysis. In a rapid response to the COVID-19 pandemic, the Seeger lab...lab demonstrated they could use the protocol to rapidly identify and isolate sybodies targeting the SARS-CoV...

  7. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...revolution in genome engineering, with new techniques rapidly being developed. Performing a CRISPR experiment...entry of a DNA template. Other techniques, such as lipid-mediated transfection (B in the figure above), are... Liu’s Lab has demonstrated the use of cationic lipid-mediated methods to deliver Cas9-gRNA RNPs to hair...been acquired, genomic editing can be performed rapidly and, in many cases, with reduced chance for off-target...PMC4938170. 2. Zuris, John A., et al. "Cationic lipid-mediated delivery of proteins enables efficient ...

  8. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    Published
    May 2, 2019, 12:41 p.m.
    ...EDGE program solicitation requires funded labs to rapidly disseminate protocols, methods, data, and tools... organisms) -  perhaps you’ll choose to study Crepidula atrasolea or Prochlorococcus with your next project...used to study aspects of cognition. Spiralians - Crepidula atrasolea (Jonathan Henry) This black footed slipper...real bottleneck. New gif for #MolluscMonday: #Crepidula atrasolea juvenile, light microscope with polarizing...are using a cool fluorescent indicator system to rapidly identify recombinants. Arthropods (Jason Rasgon...

  9. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...membrane-permeable pharmacological agent that can rapidly induction heterodimerization of proteins that contain... the mitochondria (binding FRB), allowing for a rapid and inducible inactivation. Find the Robinson plasmids...approach to control for these effects, drawing on the rapidly growing field of optogenetics. Using a rational... J 2014 Aug 1;33(15):1713-26. RUSH & CRUSH – rapid & conditional gene silencing in RNAi transgenic ...CRUSH alleles off or on enables this method to rapidly address questions of tissue-specificity and cell...engineering with difficult-to-clone sequences and rapid construction of synthetic biological systems” as...colleagues, GreenGate is a cloning system for the rapid assembly of plant transformation constructs. As ...

  10. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...unprecedented ease. These new C. elegans approaches rapidly enrich for editing events without the need for ...rescued progeny, which makes screening extremely rapid. This stringent selection allowed me to optimize...built on this work, discovering that melting and rapidly cooling dsDNA repair templates dramatically boosted...editing in other organisms and systems, allowing rapid progress in a wide range of fields.   Thank you....org/10.1534/genetics.113.156075 Ward JD (2014) Rapid and Precise Engineering of theCaenorhabditis elegansGenome...

  11. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

    Type
    Blog Post
    Published
    Sept. 18, 2018, 1:33 p.m.
    ...system: A versatile in vivo fluorescent reporter for rapid quantification of base-editing and Cas9 cleavage...popularity of single base-editors has resulted in the rapid expansion of the genome editing toolkit. However...technologies across several cell types is the lack of a rapid and quantitative editing assay. To help accelerate...has deposited a reporter plasmid that allows for rapid fluorescence-based quantification of base editors...

  12. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    Published
    Aug. 4, 2020, 1:15 p.m.
    ...advancement of SARS-CoV-2 research because they provide rapid access to validated reagents, especially during ...nonprofit research institution, has been able to rapidly propagate this strain, making it available to scientists...provides. Repositories around the world facilitate the rapid pace of SARS-CoV-2 research by removing logistic...hurdles to continue making materials available rapidly to scientists (Nature Biotechnology 2020). The ...

  13. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...advantages over pharmacological inhibitors including rapidly depleting a protein-of-interest, increasing target...system as we can use it to evaluate the effects of rapid and selective degradation of protein targets in ...machinery through binding with cereblon, leading to rapid target degradation (Figure 1). Plasmids: We generated...tagged, the dTAG lentiviral plasmids can serve as a rapid means of comparing functionality of N- and C-terminal...

  14. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...article ultimately reported the development of a rapid design and prototyping pipeline that can take pathogen...the Zika virus genome with Addgene to facilitate rapid sharing of these tools with the Zika virus research...other critical needs and to further develop our rapid prototyping and sensor assembly pipeline for future...sustainability.   References 1. Pardee, Keith, et al. "Rapid, Low-Cost Detection of Zika Virus Using Programmable...

  15. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ... that CRISPR technology is consistently showing rapid development and quick adoption by the community....request and use new tools. Because there’s such rapid adoption, we thought that requests would be biased...CRISPR tools that fill needs in the community. The rapid adoption of new tools, illustrated in Figure 2, ...usefulness of popular CRISPR tools is both enduring and rapidly recognized.   Where is the CRISPR community?  Addgene...

  16. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ...Science” described in Chapter 3, and of course the rapidly changing world of genome engineering via CRISPR...implications of using these technologies, but also the rapidity of the technologies’ adoption by the scientific... also spends some time on Addgene’s role in the rapid dissemination of these technologies, proposing that...are reading this review--in the current period of rapid flux, the chapter might still feel slightly dated...

  17. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...opening and allowing positively charged ions to rapidly pass through the cell membrane and initiate an ... their experiments. Some tools turn on and off rapidly with light to control precise timing of the perturbation...activation kinetics Some channels turn on and off very rapidly in response to a single light pulse whereas others...responses (Gunaydin et al., 2010). This can affect how rapidly you can activate or inhibit a cell in succession...

  18. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...restriction enzyme site and unique primer sites for more rapid screening by restriction digest and Sanger sequencing... sites, this technique also allows for easy and rapid targeting of complex elements to specific genomic...genome engineering method in bacteria that enables rapid, automated, and high-throughput genome editing is...(ssDNA) oligonucleotides (oligos), and thereby rapidly create desired allele combinations and combinatorial... al. J. Mol. Biol. 2015. PubMed PMID: 26264872 Rapid, conditional degradation of essential proteins using...for new actuators that are non-invasive and can rapidly and reversibly stimulate neurons. Recent studies...

  19. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...Assembly Standard and New E. Coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional....2020.100135. Fonseca, João Pedro, et al. “A Toolkit for Rapid Modular Construction of Biological Circuits in Mammalian...Extensible Mammalian Modular Assembly Toolkit for the Rapid Design and Production of Diverse Expression Vectors...Abby V., et al. Modular Vector Assembly Enables Rapid Assessment of Emerging CRISPR Technologies. Cold...

  20. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    Published
    May 12, 2020, 1:15 p.m.
    ...reason this research has been able to progress so rapidly is scientific sharing. Already we’ve seen articles...and data associated with COVID-19 research have rapidly been developed. We asked the individuals behind...is giving Rutgers investigators the agility to rapidly initiate complex research projects that they would...
Showing: 1 - 20 of 181 results