...calcium sensors from Janelia (jGCaMP7) continue to be useful for monitoring neuronal activity in the form of...while maintaining high peak fluorescence) and is useful for imaging signaling activity in large populations...AAV1) Optogenetics Optogenetics continues to be a useful method for manipulating specific neurons. We recently...

...being developed and we are excited to offer these useful tools to the community. Among other new biosensor...Minimally-tagged DREADD AAV from the Richie lab are especially useful for studies in non-human primates, where fusion...to our recombinase AAV inventory. Flp is another useful recombinase that can be used in combination with...

... directly what protocols would be useful. Think GCE could be useful in your research? We definitely recommend... scientists on which protocols would be broadly useful for biomedical researchers. One way of doing that...

...continue to provide and expand this diverse and useful collection of tools so that researchers around ...Curious which viruses researchers have found the most useful so far? We crunched the numbers on our Viral Service... made in your own lab. We provide protocols and useful tips for generating your own lentivirus. Secondly...

...important neuronal signalling pathway - if you’ve got a useful tool, there’s likely someway you can apply it in...lentivirus are now available from Addgene. While super useful, these vectors require a bit of expertise and can...learn more about these tools and to tell us how useful the service has been for their experiments. This...

... more than one word should be named in the camelCaseFormat since spaces between each word can make it ...Comments on what a given line of code does are very useful for those that are just starting out, and helps...line history by recording all commands entered is useful for beginners who are still learning the basics...

...Monster RANbody doesn’t recognize the scaffold and is useful for detecting GFP. Spaghetti Monster tagged RANbodies...IgY domains. Chicken IgY domains are especially useful for multi-color staining of tissue because they...RANbodies. If you know of a nanobody that would make a useful RANbody, consider leaving a link to its sequence...

...characterized parts into a TU! What is modular cloning useful for? All of this sounds really neat — but when ...when is this type of large-scale assembly useful, you may ask? You might be surprised by how multi-purpose... promoters, enhancers, etc. MoClo is especially useful for more complex CRISPR experiments (e.g., many...

...typically used in neuroscience research (e.g., AAV) are useful for labeling the individual neurons they infect...connections. While this transsynaptic spread is useful for neuronal tracing, it has two practical limitations...neurons doesn’t reveal network connections in a useful way, but rather just labels the whole brain. To...

...potentially a photograph of yourself. A photo can be very useful in the event that you will not be physically present...readable from several feet away. It can also be useful to use two different font styles; one for your ...The Thompson Rivers University Library provides useful tips on how to craft this type of poster using ...

...quantitative colocalization analysis methods and useful image analysis software tools. Pitfalls, limitations...colocalization methods Fluorescent proteins are very useful tools, but they are not without limitations and...you find the resources discussed in this article useful for your next localization experiment and invite...

... basics of multicistronic vectors: why they are useful, how they work, and how to get started with them...exclusively be used as a means of detection; they are useful almost anytime you want to express multiple genes...independent of the 5’ cap.  IRES elements are very useful and commonly found in bicistronic vectors; however...

...human language and observed that we humans find it useful to describe and interact with the world using words...like OR and AND. These logical operators are so useful they are implemented in most materials that have...thought these were so much fun and so incredibly useful that we wanted to make them free to use by everybody...

...your (scientific) skill set and is therefore very useful for complex projects. It is also fun! I really ...practical tools like alumni networks. I find it useful to remind myself to check in regularly with collaborators... people, checking in regularly, and maintaining useful notes that remind me about the current state of...

...element found on the neck of my guitar...not really useful for a biologist you would say. The student was ...apprehend molecular complexes and is still a very useful tool.    Like a guitar hero (that I’m not), FRET...appropriate controls are required to make this method useful for dynamic experiments in which FRET changes are...

...clearance, which is useful for imaging applications; better tissue penetration which is useful for therapeutic...delivery by viral vectors like AAV, which could be useful for delivering scFvs that inhibit HIV.   References...

...making them useful for in vivo studies. These attributes make frog eggs some of the most useful tools in ...2009). Rats are larger than mice making them more useful for certain studies that involve surgery or imaging...

...resources,  we’re highlighting just a few of the many useful kits you can order from the repository: FX Cloning...favorite gene at your level of choice and should be useful for a variety of synthetic biology or protein purification...from its creators. We hope you’ll find these kits useful in your research. If you’d ever like to deposit...

...talks at iBiology which hosts an amazing amount of useful, entertaining science lab, career, and policy content..., you just need to figure out how to make it suspenseful. 4. Start preparing your talk early - like today...information will find it. Experienced speakers present useful protocols, geeky science details, products that...

...can also cross the blood-brain barrier, making it useful for applications targeting the CNS (Issa et al....Tervo et al., 2016). This functionality makes it useful for mapping neural connections. Although derived... tissue tropism, different AAV serotypes can be useful to evade neutralizing antibodies developed from...