We narrowed to 351 results for: SUA

... Because the G is only one base, the Zhang lab usually adds it when they order the oligo. If your spacer...single-allelic and bi-allelic cells. Single-allelic cells usually make up the majority in culture unless the targeting...Ran et al. (2013). As for target selection, we usually pick 3–6 guides around the region to find the most...consider using ssDNA oligo as donor template, it's usually more efficient than plasmid donor. Typical design...CRISPR protospacer target in the HR template, you usually would need to make mutations of the HR template...probably a single stranded DNA (ssDNA) oligo which usually work better than plasmids. For ssDNA oligo design...PCR reaction. The gDNA extracted in this way is usually more concentrated than using other kits like the...

...markers found within the cytosol/nucleus, or visualizing DNA instead of proteins. Some stains can diffuse...antibodies used for target marker detection are usually conjugated to a fluorophore, not every marker will...bead-activated cell sorting, or MACS. But MACS usually only allows for sorting based on a single (lineage...proliferation between cell populations can be visualized, achieved through, for example, a previously ...divisions).   Figure 4: Proliferation dyes can visualize proliferative capacity and speed of cells. (a)...hopefully learned a few new methods of target visualization beyond the simple surface labeling. Let’s briefly...

... fluorescence is no longer quenched and can be visually detected using lateral flow strips. Since the ...AIOD-CRISPR) assay – for rapid, ultrasensitive and visual detection of novel coronavirus SARS-CoV-2 Sensitivity...machines by DIY-Bio, P51 cardboard fluorescence visualizer with 9V battery Approach :This preprint uses ...cleavage. This cleavage is coupled to fluorescence visualization. In this technique, however, instead of using...the authors employ a P51 cardboard fluorescence visualizer that is powered by a 9V battery which uses a ... Upon binding, changes in fluorescence can be visualised. Another approach was to apply the same concept...

...digestion (usually 3-6bp) Restriction Site: Your chosen restriction site for cloning (usually 6-8bp) ...primer that binds to the sequence to be amplified (usually 18-21bp) When selecting restriction sites, you...the desired location in your recipient plasmid (usually in the Multiple Cloning Site (MCS)), but do not...genomic DNA or a cDNA library), roughly 18-21bp is usually sufficient to give specificity and to also be compatible...

...wavelength of light. The switch or photoconversion usually takes less than a second. The first successful ...the fluorescence the better. More fluorescence usually means more photons are being emitted, making it...studying organelle trafficking. Monomeric PA-FPs are usually preferred for studying single protein characteristics...and after photoactivation. A high contrast ratio usually indicates that a PA-FP has a low level of spontaneous...protein (mito-PAGFP) the Youle Lab was able to visualize and quantify the fusion dynamics of mitochondria... best PA-FPs used for super-resolution imaging usually have the following characteristics-high contrast...

...basic observation and dissections. Students don’t usually perform more modern techniques used in genetics...Whole mount in Situ Hybridization (WISH) for visualizing expression of genes. Double-stranded RNA (dsRNA...protocol resources. Find Sánchez lab Plasmids Visualizing gene expression via Whole Mount In Situ Hybridization...sample, several washes, and temperature changes to visualize the expression of specific genes. After riboprobe...of T7 polymerase encoded in the E. coli genome (usually controlled by IPTG). For this IPTG-induced expression...

...organisms. Furthermore, fusing FPs to another protein usually does not affect its fluorescence. Therefore, FPs...measuring the fluorescence intensity); and 2) to visualize the localization of the FP (fused to the protein...emission color (emission wavelength range) FPs are usually classified by emission color as outlined below ...Therefore, it is recommended to use monomeric FPs (usually denoted by a “m” as the first letter in the protein...Resources: FP guide at Addgene Interactive Visualization of Fluorescent Protein Properties  Fluorescence...

...gives off a signal read by the machine’s sensors. (Usually this something is a cell, but it could be debris...populations are positive for both markers, because we usually assume (0,0) to be at the intersection of X,Y. ...the researcher, based on their controls. You can usually tell where this point is by looking at the different...plot generated in Biorender! While a helpful visualization tool, a quadrant will often be too clean for...-Anacker et al., 2017, with quadrants to help visualize the different populations. Used under Creative...

...system useful for visualization of cellular extensions, such as neurons in the fly visual system, which has...endogenous fluorescence in live animals only permits visualization of two colors. Similarly, Flybow adapts Brainbow...pdb-prot5546. Richardson, Alexander, et al. "Visualizing the Fate of Transplanted K14-Confetti Corneal...Cell Deficiency." Investigative ophthalmology & visual science 59.3 (2018): 1630-1640. Sakaguchi, Richi...

...membrane, stained with antibodies, and finally visualized.   This process allows you to detect a single... the proteins are immobilized onto a membrane, usually composed of a nitrocellulose or polyvinylidene ... Stuart Orkin's and Daniel Bauer's labs.    Visualizing your protein of interest with the help of secondary...secondary antibodies In order to visualize proteins on the membrane the antibodies are typically conjugated...species-specific secondary antibody is used to visualize the proteins. In this set-up, multiple secondary...

... cover some screening methods to identify them. Visual screening Regardless of the RNAi-trigger type you...As a plant researcher, the traditional hpRNA and visual screening method was limited in two substantial...labor-intensive processes to isolate RNAi plants. With a visual reporter of RNAi, phenotypes, such as morphological...specific target required physical isolation of the visually identified subpopulation. For traditional cell... analysis. Instead, microscopic plants must be visually identified as undergoing RNAi through the fluorescent...

...protein is targeted with an antibody, you can visualize the protein via fluorescence or chemiluminescence...and tissue labeling Antibodies can be used to visualize tissues (immunohistochemistry) or cells (immunocytochemistry...preserved, this technique allows you to not only visualize the presence of specific proteins, but their localization...contextualize its localization. These could be visualized using fluorophore-conjugated antibodies (i.e.... using an unconjugated primary antibody, then visualized using a fluorophore-conjugated secondary antibody...

...for direct visualization while epitope tags are used for both direct and indirect visualization. Some of...alternative to amylose resin and also provides a visual detection mechanism via applications such as western...affinity tags can sometimes be repurposed for visualization. However, due to the nature of the size, structure...

... has been incorporated into the host organism, usually achieved with the proper selection marker or antibiotic... fluorescent fusion protein will allow you to visualize YGOI in vivo (try pcDNA3-EGFP). If you can get...various markers and promoters. If you need to visualize YGOI on a western blot or under a microscope, ... Pick your promoter, selection cassette and visualization vehicle to conduct your custom experiment. Fly...

...The first read At the first read of the paper, I usually try to grasp the main message of the work. I underscore...the given context. After this first read, I am usually able to construct the beginning of my review - ...another day - preferably the following morning - as usually deadlines are pressing. During this second read... (3) development of tools for data analysis/visualization using R/Shiny. She is passionate about open ...

... then its concentration is detected through visualization of an enzymatic reaction. Types of ELISA There...its substrate is added and the reaction can be visualized via a colorimetric reaction. The direct ELISA...secondary antibody is conjugated to an enzyme for visualization. The indirect ELISA is used for similar experiments...additional amplification step, which helps to visualize low-abundance proteins. Figure 4: Overview...

...protein tag for visualization (Fig. 1b). While the probes target chromatin to visualize DNA, an editor ...fluorescent protein can still be included for visualization purposes, but the key component of the editing...result in depletion of the FP, which can be easily visualized.  The tool-kit consists of highly useful vectors...

...The ocean is full of striking visuals from fluorescent jellyfish, bioluminescent phytoplankton, and colorful...shorter history. In 1987, scientists described an unusual blue-colored protein from the jellyfish Cassiopea...methods like sequencing are more definitive, but visual identification is a good place to start before ...FRET References Blanquet RS, Phelan MA (1987) An unusual blue mesogleal protein from the mangrove jellyfish...

...replication, selection, and expression. Replication It’s usually not enough to get your vector into your target ...both target species. Fun fact! Plant vectors are usually designed to shuttle between E. coli and Agrobacterium...a reporter, such as a fluorescent protein, to visualize vector uptake. These reporters might be used as... as markers for automated cell sorting or to visually select cells in a microscopy image for individual...

...proteins like mScarlet are essential tools for visualizing biological processes in vivo. However, mScarlet...will be a useful addition to the toolkit for visualizing cellular processes in C. elegans: mScarlet3 for...Figure 5). The researchers used the reporter to visualize biologically relevant forces at focal adhesions...colocalizes with the focal adhesion marker paxillin (visualized with GFP). Reprinted with permission from Zhong...