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Showing: 21 - 40 of 74 results

  1. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ... cells (e.g., a neuron subtype) and specific subcellular compartments for measurements. All of the sensors...Localization The calcium concentrations in different subcellular compartments can vary greatly. Additionally, ...

  2. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...lysosome-associated membrane protein, and following its subcellular localization overtime (Subach et al.) Monitor...particular biological event (trafficking to a subcellular location, start of gene expression, development...

  3. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ... be included in a single PCR, for example for subcloning genes from different templates. All PCRs are ...can be included at the 5’ end of either primer. Subcloning - To move a gene from one vector to another simply...

  4. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ...eukaryotic) proteins localize to chemically distinct subcellular environments, including the organelles that compose...focused on optimizing fluorescent proteins for subcellular environment. Currently, she is a SPIRE postdoctoral...

  5. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...microtubule-destabilizing drugs are impossible to target to specific subcellular regions, laser ablation can damage other cellular...reversibly control Rho GTPase activity on a global to subcellular scale, enabling precise control over vascular...

  6. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...consider the subcellular location of your protein. Proteins located in membrane-bound subcompartments, like ...

  7. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...electron microscopy (EM) tag providing superior subcellular localization of tagged proteins compared to traditional...proteins of interest for EM imaging, or targeted to subcellular regions or protein complexes for proteomic mapping...

  8. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...2021). Enhancer viruses for combinatorial cell-subclass-specific labeling. Neuron,109(9), 1449–1464.e13... P. (2021). Functional enhancer elements drive subclass-selective expression from mouse to primate neocortex...

  11. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ... are you’ve used a GFP derivative to conduct subcellular localization studies or make a reporter construct...

  14. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    Published
    May 18, 2023, 1:15 p.m.
    ...receptors are specific to isotype class or even subclass, and are part of the reason why different isotypes...

  15. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ...results always pick a single colony and do not subculture directly from glycerol stocks, agar stabs, or...

  19. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ... here!   Andrews NP,  et. al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for...

  20. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    Published
    June 24, 2014, 3:59 p.m.
    .../Phototoxicity Not susceptible Susceptible Subcellular Imaging Improving Well-established High-throughput...
Showing: 21 - 40 of 74 results