...Many thanks to our guest blogger Samantha Young. Samantha Young is a Medical Writer with a PhD in reproductive... post was contributed by guest blogger Samantha Young. The use of CRISPR/Cas9 for gene editing has expanded... in scientific content.      References 1. Cho, Seung Woo, et al. "Analysis of off-target effects of CRISPR...

...proteins protect fragile samples during cryo-EM grid plunge freezing By Rachel Leeson Cryo-EM samples can be...glutaraldehyde. Adding RvLEAMshort to the sample before plunge freezing represents a convenient, effective approach...protectant for fragile samples during cryo-EM grid plunge freezing. bioRxiv 2024.02.06.579238. doi: https..., J. M., Adriaens, C., Ramadoss, G. N., Shi, Q., Hung, K. L., Samelson, A. J., … & Weissman, J. S. (2021...

...genotyping options. Figure reproduced from Tsai and Joung 2016 with permission. Even though progress has...experiments move closer to the clinic (Tsai and Joung 2016). Table 2: Unbiased Genotyping Options Technique...specificities of CRISPR–Cas9 nucleases”
by Tsai and Joung 2016. Many thanks to our guest blogger Søren Hough...PMC3651036. 15. Tsai, Shengdar Q., and J. Keith Joung. "Defining and improving the genome-wide specificities...

...Montreal, QC, Canada 45.5017°N 73.5673°W – Vanessa Sung Hundreds of scientists and supporters in Montreal...allies outside the scientific community. Vanessa Sung is currently a PhD student studying breast cancer...restorative justice.  Follow her on twitter @sung_vanessa. Albany, NY, USA 42.6526°N 73.7562°W – Nicholas... Astronomy. She studies how planets form around young stars and the instruments we use to study them. ...

...across the USA during this time have revealed that young scientists are aware of, and are engaged in, these...change the landscape facing all scientists, both young and established, as they embark on or continue their...along with progress over the past two years give young academic scientists a foot in the door, but we will...

...instillation is used to deliver substances directly to the lungs. It is an advanced technique, as it requires intubation...intratracheal counterpart, as both procedures target the lungs. The main differences are that intranasal is easier...upper airways and can target the airways before the lungs. This is most often used for delivery of viral vectors...

..., which is critically important, especially for young researchers. It was a really nice incubator for ...academic positions and poor grant situation. So, young researchers need to publish good publications in...transforming conventional research. So, I hope my younger colleagues may be able to develop new technologies...

...0022-1317-73-3-653. PMID 1372038. Randall, R. E., Young, D. F., Goswami, K. K., & Russell, W. C. (1987)....CRISPR/Cas9 system By Aryana Khosravani Deborah Hung’s lab has developed a novel dual-plasmid CRISPR/Cas9... in M. abscessus here! Neo D, Clatworthy A, and Hung, D. (2024). A dual-plasmid CRISPR/Cas9-based method...

..., Stelzer Y, Wu X, Czauderna S, Shu J, Dadon D, Young RA, Jaenisch R (2016) Editing DNA Methylation in...Reyon D, Bernstein BE, Costello JF, Wilkinson MF, Joung JK (2013) Targeted DNA demethylation and activation...Mendenhall EM, Williamson KE, Reyon D, Zou JY, Ram O, Joung JK, Bernstein BE (2013) Locus-specific editing of...

...Heart AAV1, AAV8, AAV9 Liver AAV7, AAV8, AAV9 Lung AAV4, AAV5, AAV6, AAV9 RPE (Retinal Pigment Epithelium...PMC4678200.  Kohara, K., Pignatelli, M., Rivest, A.J., Jung, H.-Y., Kitamura, T., Suh, J., Frank, D., Kajikawa...R.J.O., Mori, T., Finke, S., Conzelmann, K.-K., Young, J.A., and Callaway, E.M. (2007). Monosynaptic Restriction...

...advent tree displaying over 25 archaea, bacteria, fungi, and viruses. Final tree with the complete #MicrobeAdventCalendar...set (plus more!) It features archaea, bacteria, fungi, viruses, and one microbe ornament even dyed with...

...wanted to run and play with my kids like any other youngster. His parents, Kristi and Rico, had to keep him...Kurt has been running on behalf of AJ Gonzales, a young boy suffering from FOP, raising money for NORD for...

... field – Jennifer Doudna, Feng Zhang, and Keith Joung. All Addgene depositors! Addgene was mentioned specifically...dramatically and reducing off-target effects. Other young scientists discussed new CRISPR delivery systems...

...calcineurin is required for virulence in pathogenic fungi, it is an attractive drug target. Calcineurin reporters...as a drug-screening platform to identify new antifungals that target calcineurin. Current calcineurin ...

... KK-H, Zhang X, Fung AY-F, Tsoi H-W, Cai J-P, Chan W-M, Ip JD, Chu AW-H, Zhou J, Lung DC, Kok K-H, To ...

...Ahringer Alexander Stark Bas van Steensel Richard Young Yi Zhang Leonard Zon  You!     Chromatin-related.... Gong F, Chiu LY, Cox B, Aymard F, Clouaire T, Leung JW, Cammarata M, Perez M, Agarwal P, Brodbelt JS...

...role in Cas9 activity and efficiency (Sander and Joung, 2014). Thus it is essential to identify the best...//doi.org/10.3389/fcell.2018.00054 Sander JD, Joung JK (2014) CRISPR-Cas systems for editing, regulating...

...This guest post was contributed by Johnny Kung, Director of New Initiatives for the Personal Genetics...public on the implications of their work. Johnny Kung is the Director of New Initiatives for the Personal...

...modified version of the Python program described by Joung et al. 2017, which we hope to make available as ...:664–678 . https://doi.org/10.1089/hum.2019.277 Joung J, Konermann S, Gootenberg JS, Abudayyeh OO, Platt...

...retired! Conversely, cells younger than the 3rd – 4th passage are too young and should not be used for...