We narrowed to 215 results for: Ung;

... Plasmid Collections Synthetic Biology Fungal Synthetic Biology: Fungal SynBio Resources...collection of synthetic biology plasmids for use in fungus. Fungal Plasmids Search the table by keyword or sort... for use in fungus. Plasmid...

...informative events are one of the main ways budding young scholars across Europe get introduced to the cultures...organisation is to encourage iGEM alumni to mentor younger students in society and to support iGEM alumni-...and networking meetups. The society hopes to help young scientists interested in this field excel and develop...to see these biological societies engaging with young scholars. We believe it’s very important to develop...mentioned above provide strong foundations upon which young scientists can build powerful networks and we encourage...

... uracil DNA N-glycosylase (UNG) from E. coli. The resulting construct, eUNG-BE4max(R33A)ΔUGI, was given...Editor, or GBE, for its reliance on the glycosylase UNG to crease abasic sites (Zhao et al., 2021). Their...processes. Created with BioRender.com. Beyond UNG: Optimizing C → G editors Now that several labs had...mechanism: cytidine deaminase converts C to U, and UNG excises the new U to create an abasic site. But how... repair proteins fused to CGBEs Instead of using UNG, the Wei Leong Chew Lab developed two fusions of ...Yuan et al., 2021). In addition, they showed that UNG from both E. coli and C. elegans could be effective...team also demonstrated that different deaminase and UNG enzymes had different sequence preferences, which...

...either fused or endogenous Uracil DNA N-glycosylase (UNG) enzymes. Various other proteins included in different...guanosine instead, perhaps analogous engineering of UNG, which excises uracil, could allow it to recognize... et al., 2024; Yi et al., 2024). In each case, a UNG enzyme was modified to recognize T and/or C substrates...Also called an apurinic / apyrimidinic (AP) site. UNG / UDG Uracil DNA N-Glycosylase Removes uracil from...

...uracil N-glycosylase (UNG) during base excision repair. Adding a second copy of the UNG inhibitor, UGI, increases...and cytosine editing components together. Keith Joung’s lab created a dual-deaminase editor called SPACE... M. S., Rinaldi, C., Slaymaker, I. M., Yen, J., Young, L. E., & Ciaramella, G. (2020). Directed evolution...R., Langner, L. M., Hsu, J. Y., Aryee, M. J., & Joung, J. K. (2020). A dual-deaminase CRISPR base editor...A., Sawyer, C., Schwaegerle, N. D., Peraro, L., Young, L., Lee, S.-J., Ciaramella, G., & Gaudelli, N. ...

...pipette ready, push down the plunger. There will be multiple “stops” on the plunger, where you feel resistance... front view photo of a P1000 pipette shows the plunger at the top, which is used to draw and expel liquid...shows the volume adjustment ring right below the plunger. This ring changes the pipette volume. Below that...behind the numerical display or the top of the plunger of the pipette. Select the pipette tip that is ...ve reached step 8. Continuing to hold down the plunger, gently lower the pipette tip into the liquid. ...end of the tip is submerged, slowly release the plunger, allowing it to go back to its resting position...the pipette tip). Then, slowly press down on the plunger all the way to the second stop to release the liquid...

...Disinfectant may be added to prevent growth of bacteria or fungi. There are disinfectants designed to be used in ...

...to New England Biolabs by TelesisBio. Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA, Smith HO....

...Lee, J. K., Jeong, E., Lee, J., Jung, M., Shin, E., Kim, Y., Lee, K., Jung, I., Kim, D., Kim, S., & Kim,...Ramadoss, G. N., Shi, Q., Hung, K. L., Samelson, A. J., Pogson, A. N., Kim, J. Y., Chung, A., Leonetti, M. D...., Liu, M., Hibshman, G. N., Dangerfield, T. L., Jung, K., McCool, R. S., Johnson, K. A., & Taylor, D....Sousa, A. A., Harrington, L. B., Sternberg, S. H., Joung, J. K., Yildiz, A., & Doudna, J. A. (2017). Enhanced..., J. A., Khayter, C., Maeder, M. L., Reyon, D., Joung, J. K., & Sander, J. D. (2013). High-frequency off-target... Y., Sander, J. D., Reyon, D., Cascio, V. M., & Joung, J. K. (2014). Improving CRISPR-Cas nuclease specificity...., Peterson, R. T., Yeh, J. J., Aryee, M. J., & Joung, J. K. (2015). Engineered CRISPR-Cas9 nucleases ...

...Reagents (Kit # 1000000013 ) Depositing Labs: Keith Joung This kit consists of plasmids and strains used to...and nonprofits only. You may also like... Keith Joung Lab plasmids Daniel Voytas Lab plasmids Scot Wolfe... ML, Thibodeau-Beganny S, Sander JD, Voytas DF, Joung JK. Nature Protocols . 2009;4(10):1471-501. doi:...requires OPEN pools (available by request from the Joung lab) and the following reagents (listed below and...1997 ) and later modified by Pabo and colleagues ( Joung et al., PNAS 2000 ). The Oligomerized Pool ENgineering...protocol for practicing OPEN is available in the 2009 Joung Nature Protocols paper . How to Cite this Kit These... used in this publication was a gift from Keith Joung (Addgene kit # 1000000013)" For your Reference section...

...Axel Brunger 12339 pBD-0071 VCP His T7 ALS Axel Brunger 12344 pBD-0010 VCP His T7 ALS Axel Brunger 12345...Axel Brunger 12346 pBD-0013 VCP His T7 ALS Axel Brunger 12347 pBD-0006 VCP His T7 ALS Axel Brunger 12348...Axel Brunger 12349 pBD-0070 VCP His T7 ALS Axel Brunger 12350 pBD-0069 VCP His T7 ALS Axel Brunger 12351...Axel Brunger 12352 pBD-0007 VCP His T7 ALS Axel Brunger 12353 pBD-0018 VCP His T7 ALS Axel Brunger 12354...Axel Brunger 12355 pBD-0022 VCP His T7 ALS Axel Brunger 12356 pBD-0024 VCP His T7 ALS Axel Brunger 12357...Axel Brunger 12358 pBD-0035 VCP His T7 ALS Axel Brunger 12359 pBD-0039 VCP His T7 ALS Axel Brunger 12360...Axel Brunger 12361 pBD-0072 VCP His T7 ALS Axel Brunger 12362 pBD-0050 VCP His T7 ALS Axel Brunger 12363...

...TALengineering Reagents Joung Lab TAL Effector Engineering Reagents You may also like... Keith Joung Lab plasmids...Reagents from the Keith Joung laboratory for engineering TAL effectors, including designed TALENs, and... Daniel Voytas Lab plasmids CRISPR plasmids The Joung Lab has developed three platforms for engineering...pages describing these reagents are provided below: Joung Lab REAL Assembly TALEN Kit Individual REAL TALE...

...Welch MM, Sousa AA, Harrington LB, Sternberg SH, Joung JK, Yildiz A, Doudna JA. 2017. Enhanced proofreading...: 26456817 Fu Y, Sander JD, Reyon D, Cascio VM, Joung JK. 2014. Improving CRISPR-Cas nuclease specificity...Pattanayak V, Prew MS, Tsai SQ, Nguyen NT, Zheng Z, Joung JK. 2016. High-fidelity CRISPR-Cas9 nucleases with...Gonzales AP, Li Z, Peterson RT, Yeh JR, Aryee MJ, Joung JK. 2015. Engineered CRISPR-Cas9 nucleases with ...Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin EV, Zhang F. ...

...in multiple organisms. Dan Voytas Adam Bogdanove Joung Lab TAL Effector Engineering Reagents Assembly via...ligation. Validated in zebrafish somatic cells. Keith Joung Zhang Lab TALE Toolbox PCR/Golden Gate cloning method... cloning (LIC). Validated in human cells. Veit Hornung Musunuru/Cowan Lab TALEN Kit Kit comprised of 834... and mouse pluripotent stem cells. 48706 pTAL7b Joung Lab REAL Assembly TALEN Kit 49042 EMM65 Bradley ...

...deposited by Zinc Finger Consortium members like the Joung Lab, Voytas Lab, and Wolfe Lab... Consortium Reagents You may also like... Keith Joung Lab plasmids Daniel Voytas Lab plasmids Scot Wolfe...zinc finger technology. Consortium members Keith Joung and Daniel Voytas have deposited at Addgene various...

...interest, including bacteria, viruses, protozoa, fungi, and more. ...bacteria, viruses, parasites such as protozoa, and fungi. Find plasmids below for the species you work with...Plasmids for Cyanobacteria Cyanobacteria Plasmids for Fungi Species Aspergillus sp. Cryptococcus sp. Plasmids...

...best-selling kit in Addgene’s history. Dr. Keith Joung’s lab at Massachusetts General Hospital also recently...serial ligation protocol to assemble arrays. Dr. Joung, co-founder of the Zinc Finger Consortium with Dr...Sander JD, Cade L, Khayter C, Reyon D, Peterson RT, Joung JK, Yeh JR. Nat Biotechnol. 2011 Aug 5;29(8):697...

...explains. Elizabeth Unger: Developing a serotonin sensor The goal of Elizabeth Unger’s postdoctoral work...Commemorative Travel Awards: Melissa Chiasson, Elizabeth Unger, and Michal Bernach. Melissa Chiasson: Quantifying... fluorescent sensor for serotonin. According to Unger, “serotonin is a well-known but poorly studied neuromodulator...incredibly important for nearly every aspect of life.” Unger began with the a bacterial periplasmic binding protein...no known bacterial proteins that bind serotonin. Unger redesigned the binding pocket of the periplasmic...for serotonin by over four orders of magnitude. Unger validated the serotonin sensor with proof-of-principle...can also kinetically outperform current methods. Unger plans to use the sensor to answer the question of...

...mutated genes in lung adenocarcinoma. Delivery of a single AAV vector ( AAV-KPL ) in the lung generated loss-of-function...complex. Konermann S*, Brigham MD*, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg...

... on the lungs, and, among other projects, using stem cells to develop in vitro models for lung disease...goal - to develop therapies to treat disease in the lung. Chapter 2: Balancing research with medical practice...used to better understand the biology of certain lung diseases. Chapter 6: Near-term possibilities for...for using engineered cells in therapy. The lungs are difficult, but other tissues like the retina have ...therapies underway. Chapter 7: Generating entire lungs using a decellurized scaffold as a base. Darrell... using stem cells from patients to study various lung diseases. Chapter 10: How Darrel defines Open Science...