We narrowed to 204 results for: Xpa
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TypeBlog PostPublishedJuly 27, 2021, 1:15 p.m....physiology, development, and disease. In order to expand their model’s impact, organoid users have recently...months: Our selection of GCaMP8 AAV preps has expanded. Check out the new selection of ready-to-use AAV9... INTRSECT 2.0 calcium sensor available in AAV8! Expand your imaging experiments with the Cre-OFF/FLP-ON...
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New and Upcoming Viral Vectors - December 2019
TypeBlog PostPublishedDec. 19, 2019, 2:40 p.m....for light mediated neuronal control and we are expanding our viral service to make these readily available... experiment. The Addgene viral service has now expanded to offer many of our control plasmids in new serotypes... is vital in studying neural networks. We have expanded our calcium sensor collection to include our first... -
15 Hot Plasmids from 2017
TypeBlog PostPublishedJan. 9, 2018, 3:02 p.m....).This toolbox adds new neuronal tools to the expanding gateway compatible vectors currently shared amongst...Podcast Segment Photocleavable protein PhoCl expands optogenetics toolbox Increasingly popular Optogenetics...proteins (FP) for cellular imaging is constantly expanding. Case in point - check out the growing list of...new base editors with distinct PAM sequences, expanding the number of available target sites for base ... -
Addgene’s Viral Service - Why Virus? Why Now?
TypeBlog PostPublishedMarch 23, 2017, 2:30 p.m....hundreds of scientists about various ways we might expand Addgene’s services. There was an overwhelmingly... to facilitate the formation of partnerships to expand the service and figure out what we can afford to...educational content via the Addgene Blog and our expanding website resources. I hope this gave you the impression... -
Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity
TypeBlog PostPublishedNov. 5, 2019, 1:28 p.m....Our previous blog post The PAM Requirement and Expanding CRISPR Beyond SpCas9 described the PAM specificity...toolkit available to researchers will continue to expand. Final thoughts Since the first use of Cas9 to ..., et al. "Engineered CRISPR-Cas9 nuclease with expanded targeting space." Science 361.6408 (2018): 1259... -
Addgene: The First Twenty Years
TypeBlog PostPublishedDec. 19, 2024, 2:15 p.m....celebrating Addgene's 10 year anniversary. Expanding our work In March 2015, Addgene reached the 500,000...over 114,600 viral preps. And in 2025, we’ll be expanding our viral vectors service to include AAV Packaged...slowed, Addgene began to think about how we could expand our support for the research community once more... -
CRISPR 101: Cas9 vs. The Other Cas(s)
TypeBlog PostPublishedDec. 8, 2022, 2:15 p.m....enzyme. Fast facts – PAM requirement: NGG and an expanding number of others. Best for: a wide range of DNA...scientist toolbox. If your genome engineering goals expand beyond generating frameshift mutations or single...my CRISPR experiment? The PAM requirement and expanding CRISPR beyond spCas9 Cas proteins More resources... -
CRISPR-mediated Plant Base Editors
TypeBlog PostPublishedJan. 3, 2019, 1:35 p.m....plant base editors. Plant CBE and ABE with expanded PAM compatibility All of the base editors mentioned.... Hua, Kai, Xiaoping Tao, and Jian‐Kang Zhu. "Expanding the base editing scope in rice by using Cas9 variants.... PubMed PMID: 29867128. 11. Li, Chao, et al. "Expanded base editing in rice and wheat using a Cas9-adenosine... -
Transferable Skills Guide: External Collaborations
TypeBlog PostPublishedApril 19, 2018, 1:25 p.m....below). Expand: Collaboration opportunities outside an organization The next step is to expand your efforts... to start close to your comfort zone and slowly expand from there. Start small: Collaboration opportunities... -
Using Phosphoserine to Study Protein Phosphorylation
TypeBlog PostPublishedJune 23, 2016, 2:30 p.m....modified through phosphorylation (1). However, the expansion of studies on protein phosphorylation in an array...PubMed PMID: 15790858. 5. Park, Hee-Sung, et al. "Expanding the genetic code of Escherichia coli with phosphoserine... Marc J., et al. "Genomically recoded organisms expand biological functions." Science 342.6156 (2013):... -
Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands
TypeBlog PostPublishedMarch 21, 2024, 1:15 p.m.... of more stable, brighter, and specific dyes, expanding the capabilities of fluorescence-based techniques...® system, the new Janelia Fluor HaloTag Ligands expand the self-labeling capabilities of this platform...Rampioni, G., Visca, P., & Kolmakov, K. (2023). Expanding the microbiologist toolbox via new far-red-emitting... -
The Developmental Studies Hybridoma Bank: Over 25 Years of Antibody Sharing
TypeBlog PostPublishedMarch 10, 2015, 2:25 p.m....called hybridomas, are individually isolated and expanded. The clonal cells are screened to identify hybridoma... Addgene's First 10 Years Data Freedom: The Expansion of Data Sharing in Research Publications ... -
2022 and 2023: Reflections and Looking Forward
TypeBlog PostPublishedFeb. 23, 2023, 2:15 p.m....information sharing, and research reproducibility while expanding our suite of materials and services to support...many exciting things planned in 2023, from the expansion of our Data Hub to process improvements in every... -
Phage Directory: From Phage Therapy to a Repository of Phage Information
TypeBlog PostPublishedJan. 10, 2019, 2:38 p.m....year ago, Sacher and Zheng found that they could expand their role in the phage community by being a repository...Sacher says. Sacher hopes that as Phage Directory expands, it can also fill this role by cataloging and archiving... -
Early Career Researcher Toolbox: Free Online Molecular Biology Tools
TypeBlog PostPublishedSept. 12, 2023, 1:15 p.m....or protein sequences to sequences in a database ExPASy translation tool: Translates a DNA sequence using...for fluorescent proteins and their properties. Expasy: Bioinformatics resource portal which includes ... -
Embracing Serendipity: A Crucial Element in the PhD Journey
TypeBlog PostPublishedApril 25, 2024, 1:15 p.m....inviting serendipity into your research lies in expanding the scope of your exploration. While it is essential...diverse literature. By delving into new topics and expanding your knowledge base extensively, you open doors... -
Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools
TypeBlog PostPublishedSept. 18, 2018, 1:33 p.m....single base-editors has resulted in the rapid expansion of the genome editing toolkit. However, a challenge...quantitative editing assay. To help accelerate the expansion of genome editing tools, the Reuben Harris lab... -
Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource
TypeBlog PostPublishedMay 25, 2021, 2:29 p.m....neuroscience reagents and data, the platform can be expanded for any recombinant antibody tool and therefore...distributions to cover costs, which can limit expansion into new services. “We appreciate NIH’s vision... -
Extracurricular Activities for a Strong Science Career Path
TypeBlog PostPublishedJan. 21, 2014, 3:08 p.m....Participating in activities outside of the lab expands your professional network. No brainer! Engaging...spent away from the bench, but as an opportunity to expand associations outside of the traditional academic... -
New and Upcoming Viral Vectors - September 2019
TypeBlog PostPublishedSept. 12, 2019, 1 p.m....AAV inventory for the entire collection! We are expanding our entire control AAV inventory to give scientists...Overview of AAV Encoded Calcium Sensors). We are expanding the inventory of these vectors to more serotypes...