We narrowed to 39 results for: cDNA
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TypeBlog PostPublishedJuly 13, 2023, 1:15 p.m....types of pooled libraries here at Addgene, including cDNA, barcoding, screening, and gRNA libraries. Most ...
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Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus
TypeBlog PostPublishedMarch 17, 2016, 2:30 p.m....of bovine respiratory syncytial virus (BRSV) from cDNA: BRSV NS2 is not essential for virus replication... -
Tips for Screening with Yeast Two Hybrid Systems
TypeBlog PostPublishedOct. 22, 2015, 2:30 p.m....fragments from the organism of interest, or a defined cDNA library (e.g. the entire proteome of a particular... -
Fluorescent Proteins 101: When GFP lets you down
TypeBlog PostPublishedAug. 23, 2018, 12:05 p.m....proteins are made by connecting its DNA code to the cDNA encoding for another protein. Effectively, it equips... -
SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas
TypeBlog PostPublishedMay 5, 2020, 1:15 p.m....reactions also contain reverse transcriptase to produce cDNA. Isothermal amplification is done at one constant... -
Plasmids 101: Using Transposons in the Lab
TypeBlog PostPublishedJuly 6, 2021, 1:28 p.m....for lentivirus). Large cargos, such as the ~11 kB cDNA for dystrophin, the gene mutated in muscular dystrophy... -
Plasmids 101: Modular Cloning Applications and Kits
TypeBlog PostPublishedMay 14, 2024, 1:15 p.m...., solubility and purification tags, connectors, cDNAs, and more to find the perfect combination. The EcoFlex... -
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation
TypeBlog PostPublishedNov. 6, 2018, 1:12 p.m.... A.B. Lassar, Expression of a single transfected cDNA converts fibroblasts to myoblasts. Cell, 1987. 51... -
Delivery Methods for Generating iPSCs
TypeBlog PostPublishedApril 17, 2018, 1:37 p.m.... They contain only a eukaryotic promoter and the cDNA(s) to be expressed and they don’t integrate or replicate... -
28 Hot Plasmid Technologies from 2015
TypeBlog PostPublishedDec. 23, 2015, 3:30 p.m....constitutive (CMV, SV40, SFFV) or inducible (CMV-TO) cDNA expression, sgRNA (U6) expression, shRNA/shRNA-miR30...CreERT2 gene deletion, (3) simultaneous expression of cDNAs and shRNAs with a reporter gene, or (4) multiple...luciferase-fluorophore pH fusion reporters, pRSETb-pHlash and pcDNA3.1+-pHlash, that use Bioluminescence Resonance Energy... -
CRISPR 101: Epigenetics and Editing the Epigenome
TypeBlog PostPublishedJune 24, 2020, 5:45 p.m....mammalian expression constructs including pcDNA-dCas9-p300 Core and pcDNA3.3-Nm-dCas9-p300 Core, as well as pLV-dCas9... in mammalian cells. This plasmid is used with pcDNA3.1-MS2-Tet1-CD to decrease methylation and activate...Methyltransferase MQ1 Margaret Goodell's lab has deposited pcDNA3.1-dCas9-MQ1(Q147L)-EGFP, a fusion of dCas9 to a ... -
Choosing Your Perfect Empty Backbone
TypeBlog PostPublishedAug. 19, 2014, 3:39 p.m....mammalian expression vectors, c-Flag pcDNA3, comes from the pcDNA series. It has the CMV promoter for ...protein will allow you to visualize YGOI in vivo (try pcDNA3-EGFP). If you can get away with generating transiently... -
27 Hot Plasmids from 2016
TypeBlog PostPublishedDec. 22, 2016, 3:03 p.m....Takashi Yamamoto pcDNA-TAL-NC2, pCAGGS-TAL-NC2 Charles Gersbach pcDNA3.1-GoldenGate, pcDNA3.1-GoldenGate-...Type 74309 pcDNA3.0-TRPV4-p2A-ferritin-p2A-mCherry Mammalian Expression 74308 pcDNA3.0-Magneto2.0-p2A-mCherry... -
Illuminating Epigenetics with A FRET Based Biosensor
TypeBlog PostPublishedNov. 19, 2015, 3:30 p.m....reporter to target the K9 (pcDNA3-K9 histone methylation reporter) and K27 (pcDNA3-K27 histone methylation... -
Twenty Years of Sharing Science!
TypeBlog PostPublishedJan. 8, 2024, 3:25 p.m....Our first request was on September 8, 2004 (for pcDNA-p160MBP, if you're curious.) Since then, we've distributed... -
Export Control Regulations Lifted for Plasmids Containing Rabies and VSV-G Genes
TypeBlog PostPublishedFeb. 6, 2025, 2:15 p.m....paper for more information Miguel Sena-Esteves pcDNA-SADB19L Contains rabies viral RNA polymerase; ... -
Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools
TypeBlog PostPublishedSept. 18, 2018, 1:33 p.m.... is available in a mammalian expression vector (pcDNA3) and a bacterial expression vector (pRSETB). ... -
Addgene: The First Twenty Years
TypeBlog PostPublishedDec. 19, 2024, 2:15 p.m.... plasmid request came on September 8, 2004, for pcDNA-p160MBP, while the first request for a kit came ... -
Plasmids 101: Expressing shRNAs for RNA Interference
TypeBlog PostPublishedJune 17, 2025, 1:15 p.m....between the red boxes. (B) Plasmid sequence from pcDNA3-miR21 (Addgene plasmid #21114). The miRNA sequence...