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We narrowed to 63 results for: cDNA

Showing: 1 - 20 of 63 results

  1. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    ...region of a gene (cDNA) can be done by either plasmid or virus. A plasmid carrying your cDNA can be targeted...to integrate your choice cDNA. Both lentivirus and retrovirus can package cDNAs for genomic delivery and...to choose an appropriate promoter to express your cDNA, depending on the level of expression you would ...

  2. Plasmid Cloning by PCR

    Type
    Blog Post
    ...For this example, we will describe how to copy a cDNA from one vector into a new vector that is better...example, we will use EcoRI and NotI to ligate our cDNA into the recipient plasmid. Remember to insert your...from plasmid DNA (rather than from genomic DNA or a cDNA library), roughly 18-21bp is usually sufficient ...Tm if you are trying to clone from genomic DNA, a cDNA library, or by RT-PCR. Isolate the PCR product Isolate...

  3. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    ... set of one to three mutant complementary DNAs (cDNAs) representing core nodes that, when over-expressed...curate the perfect library, obtained the appropriate cDNAs from other researchers, and cloned them into lentiviral...months from start to finish. They cloned all the cDNAs into a single consensus lentiviral expression vector...

  4. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...simpler to implement in genome-wide screens over cDNA libraries. However, using CRISPRa to target individual...highlight a key advantage of CRISPRa over traditional cDNA overexpression: isoform-specific gene expression...— at least, more straightforward than generating cDNA for each gene isoform individually. To highlight...application can be found here.  Why CRISPRa? Although cDNA overexpression approaches may still be preferable...strategies) and then consider whether traditional cDNA overexpression is suited to your experimental goals...

  5. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type
    Blog Post
    ...large scale, broadly available collection of mutant cDNA clones corresponding to cancer variants found in... genome sequencing studies (as well as reference cDNA clones mapping to well-annotated cellular pathways...Addgene. These include 634 sequence confirmed mutant cDNA clones corresponding to cancer variants derived ...

  6. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...For this example, we will describe how to copy a cDNA from one vector into a new vector that is better...example, we will use EcoRI and NotI to ligate our cDNA into the recipient plasmid. Remember to insert your...from plasmid DNA (rather than from genomic DNA or a cDNA library), roughly 18-21bp is usually sufficient ...Tm if you are trying to clone from genomic DNA, a cDNA library, or by RT-PCR. Isolate your PCR product ...

  7. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...they want to activate, we would recommend using a cDNA overexpression vector rather than going through ...protein structure and function (Pan et al., 2018). cDNA libraries, which consist of plasmids that over-express...and deliver when compared to gRNAs. Additionaly, cDNAs cannot be used to study in cis regulation and also...

  10. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type
    Protocol
    ...purposes of this tutorial we will discuss how to move a cDNA from one plasmid to another. However, the same technique... problem is that the only version of full-length cDNA you can find for YGOI is in a bacterial expression...

  11. Lentivirus Plasmids

    Type
    Collection
    ...expression of cDNA. Puro selection. Shih 25737 pSLIK-Hygro 3rd Tet-based inducible shRNA or cDNA expression...EGFP 3rd CMV-driven EGFP fusion; can be used for cDNA expression; puro resistance Sabatini 14883 FUGW ...FUGW 3rd hUbC-driven EGFP; can be used for cDNA expression Baltimore 12247 pLVTHM 2nd EF-1a-driven GFP and...plasmid with constitutive or inducible expression of cDNAs, shRNAs or miRNAs, and use a wide variety of drug...3rd Tet inducible gateway destination plasmid for cDNA expression Ramalho-Santos 24129 pULTRA 3rd bi-cistronic...3rd Gateway plasmid for CMV driven expression of cDNA. See article for alternative versions of this plasmid...21373 pHIV-EGFP 3rd EF-1alpha driven expression of cDNA and and EGFP co-expression. See plasmid 21374 for...
Showing: 1 - 20 of 63 results