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We narrowed to 39 results for: cDNA

Showing: 1 - 20 of 39 results

  1. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    Published
    Feb. 16, 2023, 7:05 p.m.
    ...region of a gene (cDNA) can be done by either plasmid or virus. A plasmid carrying your cDNA can be targeted...to integrate your choice cDNA. Both lentivirus and retrovirus can package cDNAs for genomic delivery and...to choose an appropriate promoter to express your cDNA, depending on the level of expression you would ...

  2. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ...For this example, we will describe how to copy a cDNA from one vector into a new vector that is better...example, we will use EcoRI and NotI to ligate our cDNA into the recipient plasmid. Remember to insert your...from plasmid DNA (rather than from genomic DNA or a cDNA library), roughly 18-21bp is usually sufficient ...Tm if you are trying to clone from genomic DNA, a cDNA library, or by RT-PCR. Isolate the PCR product Isolate...

  3. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    Published
    March 22, 2016, 2:30 p.m.
    ... set of one to three mutant complementary DNAs (cDNAs) representing core nodes that, when over-expressed...curate the perfect library, obtained the appropriate cDNAs from other researchers, and cloned them into lentiviral...months from start to finish. They cloned all the cDNAs into a single consensus lentiviral expression vector...

  4. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    ...simpler to implement in genome-wide screens over cDNA libraries. However, using CRISPRa to target individual...highlight a key advantage of CRISPRa over traditional cDNA overexpression: isoform-specific gene expression...— at least, more straightforward than generating cDNA for each gene isoform individually. To highlight...application can be found here.  Why CRISPRa? Although cDNA overexpression approaches may still be preferable...strategies) and then consider whether traditional cDNA overexpression is suited to your experimental goals...

  5. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type
    Blog Post
    Published
    May 11, 2017, 2:30 p.m.
    ...large scale, broadly available collection of mutant cDNA clones corresponding to cancer variants found in... genome sequencing studies (as well as reference cDNA clones mapping to well-annotated cellular pathways...Addgene. These include 634 sequence confirmed mutant cDNA clones corresponding to cancer variants derived ...

  6. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...they want to activate, we would recommend using a cDNA overexpression vector rather than going through ...protein structure and function (Pan et al., 2018). cDNA libraries, which consist of plasmids that over-express...and deliver when compared to gRNAs. Additionaly, cDNAs cannot be used to study in cis regulation and also...

  12. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...expression construct, to cells/organisms. This is often cDNA, shRNA, or something similar, and is contained on...

  13. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ...In this approach, viral RNA is first converted to cDNA and then quantified using qPCR primers targeting...

  18. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ... created for a variety of applications including cDNA expression, shRNA-mediated knockdown, Tet and Cre-regulated...

  19. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...of these species and can often be used to clone cDNAs from them. These enzymes might not, however, be ...

  20. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ...types of DNA fragments may be cloned: PCR fragments, cDNA or Genomic DNA and is available for all kind of ...
Showing: 1 - 20 of 39 results