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We narrowed to 137 results for: ckb

Showing: 21 - 40 of 137 results

  1. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ... resistances, restriction enzymes and plasmid backbones. pUCXKT contains a nonfunctional truncated version...site in pUCXKT, removing the stop codons during backbone digestion and providing the missing codons for... both kanamycin and ampicillin (from the pUC19 backbone). *False positives can occur in this system if...lentivirus production Finding a lentiviral vector backbone with just the right combination of promoter, cloning...create the pCell-Free vectors, Gateway-compatible backbones which enable cell-free expression of proteins ...originating from cryptic promoters in the plasmid backbone (Giannaks et al 2003, Bert et al 2000). In 2000...

  2. An Addgene Summer Internship

    Type
    Blog Post
    Published
    Aug. 8, 2024, 7:22 p.m.
    ... cloning the Syntaxin3 gene in a pLenti vector backbone. During those five weeks, I worked with my mentors...had successfully incorporated Syntaxin3 into its backbone. Once we had gotten our results back from the ...successful clone of the Syntaxin3 in the pLenti backbone! Once everything was confirmed, I worked on my...

  3. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type
    Blog Post
    Published
    Aug. 28, 2014, 3:34 p.m.
    ...particular vector by excising the insert from the backbone. To do this, you'll use enzymes with restriction...to know both the approximate size of the vector backbone as well as the predicted size of the insert. You...bands at 6kb and 1.2kb (red box), matching the backbone and insert, respectively. The results on the gel...

  4. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ... NGS result to a reference sequence to confirm backbone elements.   Confirming the gene/insert by aligning...NGS result to a reference sequence like a known backbone sequence. We’re not necessarily expecting a perfect...mismatches in the origin of replication or other common backbone elements. Since we’ve successfully grown the plasmid...

  5. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ...vector backbone from the Shih lab. You can find more lentiviral expression vectors (empty backbones) here...Plasmid #52961) This is a 3rd generation lentiviral backbone from the Zhang lab expressing S. pyogenes Cas9...

  6. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type
    Blog Post
    Published
    May 24, 2018, 1:20 p.m.
    ...species. These plasmids all have a PR-T4 vector backbone, which is a modified version of L440. This vector...cloning to insert the cDNA into the desired plasmid backbone. The Sánchez lab plasmids allow students and new... gene of interest into the empty PR-T4P vector backbone. The empty vector PLL4, which is similar to PR-T4P...

  7. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ... of the essence. This method requires a vector backbone containing a topoisomerase I cut site, toposisomerase...the topoisomerase I generated overhangs in the backbone. Once combined, the cut and ligation processes...out protocols for plasmid cloning Find vector backbones Read our molecular biology reference ...

  8. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...expressed from different plasmid backbones. They noted that backbone differences such as the order, or...used to assess how different lentiviral plasmid backbone elements impact viral titer and to compare different...

  9. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    Published
    Jan. 11, 2017, 8:47 p.m.
    ...peptides. PX459 This is the vector backbone. This particular backbone can be used to transfect a variety...

  11. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    Published
    Sept. 12, 2023, 1:15 p.m.
    ...links can help you calculate how much insert and backbone you need for different types of cloning methods...method. You can select from five different insert:backbone ratios. In-Fusion cloning molar ratio calculators...

  12. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    Published
    April 25, 2023, 1:15 p.m.
    ...parental antibody (blue, rat) are cloned into a backbone expressing the constant regions from an alternative... a specific antibody are cloned into a plasmid backbone containing all constant regions, just the heavy...

  13. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...Check Out Addgene's Empty Backbone Resource: Choosing Your Perfect Plasmid Backbone Read More: Plasmids...

  15. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ...create an amplicon that  was cloned into a plasmid backbone for expression of the mAb heavy and light chain...modify the CH encoding sequence within the plasmid backbone to generate R-mAbs of different subclasses, including...

  18. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    ...blue-white screening Use a good control: Transform the backbone plasmid without insert. All colonies on this plate...list of Bacterial Expression Systems Find Empty Backbones for Your Experiments    ...

  19. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ...while expressing the gene. Briefly, the vector “backbone” in this system contains ccdB. A successful insertion...propagate and prep your ccdB-containing empty backbones but should never be used when selecting for recombinant...

  20. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ...that target the common features of viral vector backbones; once validated these universal primers can be... for functional assays depending on the vector backbone. When using qPCR based methods for viral titration...
Showing: 21 - 40 of 137 results