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Showing: 21 - 40 of 683 results
  1. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles.  ... or want to swap out the constant domains, the plasmids for the heavy chain and light chain are also available...minimal background.   Find these C. elegans mScarlet plasmids here!   Cao W.X., Merritt D.M., Pe K., Cesar ...Neo et al., 2024 under CC-BY license.   Find plasmids for CRISPR/Cas9 in M. abscessus here! Neo D, ...with a time resolution of minutes.  Find PILATeS plasmids here! Zhong, B. L., Elliot, J. M., Wang, P., ...
  2. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...of the new plasmids, antibodies, and viral preps in our repository through our Hot Plasmids articles. ...be used for applications beyond cryo-EM.   Find plasmids for MagIC-Cryo-EM here!   Arimura, Y., Konishi...disease phenotype in an animal model. Find PE-eVLP plasmids here! The lab’s other recent deposit builds on...over the wild-type enzyme. Find optimized PASSIGE plasmids here!     Figure 3: Schematic of the PASSIGE...without compromising their efficacy.   Find CHARM plasmids here!   Neumann, E. N., Bertozzi, T. M., Wu, E...
  3. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...of the new plasmids, antibodies, and viral preps in our repository through our Hot Plasmids articles.  ...including in human retinal organoids and live mice. Importantly, REVeRT avoids the introduction of extraneous... over the original CROPseq. Find CROPseq-multi plasmids here Walton, R.T., Qin, Y., Blainey, P.C. (2024...potential improvements to the editor. Find PE7 plasmids here!     Figure 2: PE7 improves prime...conjugated with its HaloTag domain.  Find Voltron2 plasmids here! Abdelfattah, A.S., et al. (2023) Neuron...therapeutic applications.  Find microglia-targeting AAV plasmids here! Serrano, C., et al. (2023). Simple and ...potential for therapeutic success. Find REVeRT plasmids here! Riedmayr, L.M., et al. (2023). mRNA trans-splicing...
  4. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...on Addgene.org CRISPR Plasmids – Protein Tagging CRISPaint Gene Tagging Plasmids 101 eBook Resources ...Resources on the Addgene blog Plasmids 101: Protein Tags Plasmids for Endogenous Gene Tagging in Human Cells ...
  5. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ...NEB’s DAD Interview with Nicola Patron on MoClo Plasmids 101: Golden Gate Cloning ...
  6. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles.  ... al. 2023 with permission.   Find Cas-PAGE plasmids here!   Zhang, Z., et al. (2023). Efficient engineering...inspired and informed this section.  Find mStayGold plasmids at Addgene here!   Ando, R., et al. (2023). StayGold... primate (NHP) brains.  Systemic gene delivery capsids previously identified in rodents have not been ...Addgene’s plasmid collection includes over 10,000 plasmids with a FLAG tag (!), but did you know that we ...
  7. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    Published
    Feb. 1, 2024, 2:15 p.m.
    ... ship rabies plasmids and viral vectors containing genetic elements from rabies plasmids to international... materials in our repository, including rabies plasmids, are export-controlled materials in the USA, where...export license applications for VSV and SARS-COV-1 plasmids. Export control license process    An export ...license, once approved, allows Addgene to ship plasmids or viral vectors containing sequences from rabies...  Timelines  We have offered VSV and SARS-COV-1 plasmids under this application process since May 2020....at [email protected].  Curious about how rabies plasmids are used in the lab? Check out our “Rabies and...browse tools for AAV tracing, made from rabies plasmids deposited by Ian Wickersham’s lab.   ...
  8. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles. .... Today, it's time for another edition of Hot Plasmids!  Here's what you'll find in this post:  New...., 2023 under CC-BY 4.0 License. Find PE6 plasmids here!  Doman, J.L., Pandey, S., et al. (2023)....therapies and medical applications.  Find DIRECTED plasmids here! Strebinger, D., et al. (2023). Cell type-specific...pulse-chase experiments, and more. Find LOV-Turbo plasmids here! Next, Qin and Cheah et al. described TransitID...LOV-Turbo into TransitID experiments.  Find TransitID plasmids here! These two developments open up new possibilities... promoter activity in single cells. Find iPAK4 plasmids here! Lin D., Li X., et al. (2023). Time-tagged...
  9. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...of the new plasmids, antibodies, and viral preps in the repository through our hot plasmids articles. ... missed something smokin'? You can pitch a Hot Plasmids section (antibodies and viral preps welcome!) ...Figure 3 Colognori et al 2022.   Find CRISPR Csm plasmids here! Colognori D, Trinidad M, Doudna JA. Precise...inducible expression.   Find heat-inducible lentiviral plasmids here!   Wu Y, et. al. Control of the activity ... Campbell et al, Nature Methods   Find YFP plasmids here!   Campbell, B.C., et al. Chemically stable...
  10. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...Addgene blog:  Plasmids 101: What is a plasmid? Plasmids 101: Origin of ReplicationPlasmids 101: PCR... the sequencing results for their plasmids, they found the plasmids were much longer than expected, and...When performing restriction digests on plasmids at Addgene, we sometimes observe something odd in our...full plasmid sequencing services to verify the plasmids, including long-read sequencing techniques, such...was repeated two or more times in tandem. Such plasmids are known as multimers, and they are the result...been observed to occur in many different kinds of plasmids, taking many forms (Levene, 2009; Higgins & Vologodskii... has been observed to occur more frequently in plasmids with large inserts (>7 kb)(Berg et al., 1989),...
  11. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...Tet-Off system.  For more information on Addgene Tet plasmids, see our Tetracycline resource page. Cumate Switch...20697123. PubMed Central PMCID: PMC2942869. Find plasmids from this publication at Addgene. del Valle Rodriguez...20434990. PubMed Central PMCID: PMC2883883. Find plasmids from this publication at Addgene. Wei, Xing, ...22406855. PubMed Central PMCID: PMC3846601. Find plasmids from this publication at Addgene. Riabinina, ...25581800. PubMed Central PMCID: PMC4344399. Find plasmids from this publication at Addgene. Riabinina O...
  12. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...Resources on the Addgene Blog: Plasmids 101: The Promoter Region Plasmids 101: Cre-lox Light-Switchable...Systems for E. coli Browse other blog posts from our Plasmids 101 series Resources on Addgene.org: Inducible...Inducible Systems at Addgene Cre-lox Guide and Plasmids     ...
  13. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...editing for precise genome modification. Plasmid ID Plasmid Name Cas9 Variant Speacial Features 85169...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These brief articles... you to find and use the plasmids you need. You can find all the hot plasmids from 2017 below. With over...write about your plasmids in a future blog post. No time to read? Listen to our hot plasmids segment on the...articles provide a synopsis of a plasmid or group of plasmids' functions and applications. We hope that these...over 50,000 plasmids, we can't write posts for every great plasmid that comes into the repository, but...this cell type. To this end, they constructed AAV plasmids bearing one of the distal-less homeobox 5 and ...
  14. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    Published
    April 2, 2020, 6:17 p.m.
    ...cloning plasmids, expression plasmids, gene knock-down plasmids, reporter plasmids, viral plasmids, and ...plasmid lovers - serves as an introduction to plasmids. Plasmids 101 will provide you with an overview of ...cloning site. The ease of modifying plasmids and the ability of plasmids to self-replicate within a cell ...bacteria from which plasmids are isolated grow quickly and make more of the plasmids as they grow, scientists....     How do scientists use plasmids? Generally, scientists use plasmids to manipulate gene expression...and genome engineering plasmids. Some of the many things that plasmids can be used to do include: Produce...quantity inside a cell Plasmids 101: Green Fluorescent Protein (GFP) Plasmids 101: Luciferase Monitor...
  15. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ...inability of different plasmids to be maintained in one bacterial cell. In this Plasmids 101 post, we’ll cover...First, why are plasmids incompatible? Plasmid incompatibility occurs when multiple plasmids within one cell...Replication? See our Plasmids 101: Origin of Replication blog).  The replication problem Plasmids with the same...with low copy plasmids, though the partitioning problem does occur in high copy plasmids (Diaz et al.,...on each plasmid and pair the plasmids together (akin to sister chromatids coming together in eukaryotic...worth noting that plasmids can be symmetrically incompatible, meaning that both plasmids are lost from the...that is easy to use with other plasmids.  How do you know if your plasmids are incompatible? Scientists ...
  16. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...our depositors. Below are the 15 top requested plasmids over the last 15 years - the majority of these...lentiviral or CRISPR tools. How many times have these plasmids been shared in total? Just over 49,000 times! ...different areas of biology as they grow. The top 15 plasmids requested from Addgene psPAX2  - psPAX2 is a ...point, you may have noticed that many other CRISPR plasmids are from the Zhang lab. In fact, the Zhang lab...retroviral particles. It can be used with packaging plasmids such as pCMV-dR8.2 dvpr (lentiviral) or pUMVC ...: this plasmid grows more slowly than standard plasmids. Find pLKO.1 - TRC cloning vector. Tet-pLKO-puro...Addgene website Browse our CRISPR collection Find plasmids for your research  Learn about our viral service...
  17. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ...to find Gateway plasmids for your organisms of interest: Find Gateway cloning plasmids here! References...desired, recombined construct. Read our recent Plasmids 101 Post on CcdB for more information. How to ... to use the Snapgene program to design Gateway plasmids.    Figure 3: Method A to create an entry...entry clones can be used with a large variety of plasmids. Speed The Gateway system enables the generation.... Take a look at some of the Gateway Multisite plasmids available at Addgene, including the Frew Lab Multiple...MultiSite Gateway cloning kit, and MultiSite Gateway plasmids. Constant reading frame When you move a DNA fragment...generated and deposited their own Gateway-compatible plasmids with Addgene. These can be used to express genes...
  18. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...immunohistochemistry, and are summarized in the table below:   ID Cv (Capsid) FLAG Tag Terminus 79629 (N) and 79628... Neurosci 2016. PubMed PMID: 26950006 Plasmid ID Plasmid Name Plasmid Type 74309 pcDNA3.0-TRPV4-...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These brief articles...set of plasmids. We hope that these articles make it easier for you to find and use the plasmids you need...You can find all the hot plasmids from 2016 below. With over 45,000 plasmids, we can't write posts for...write about your plasmids in a future blog post. No time to read? Listen to our hot plasmids segment on the...360 Ras pathway plasmids to the scientific community. This collection consists of plasmids containing genes...
  19. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...to Easily Generate Insertion and Deletions in a Plasmids Learn How to Verify Your Plasmid Resources on...
  20. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ...step is to mini-prep these clones and submit the plasmids for Sanger sequencing. Sequencing allows you to...
Showing: 21 - 40 of 683 results