Designer PUF Proteins for Any RNA Target
Type
Blog Post
Published
June 17, 2014, 7:57 p.m.
...PUF proteins!
A newly available PUF Assembly Kit makes it possible to devise RNA binding proteins to hit...applying the Golden Gate cloning method to human proteins known as Pumilio/fem-3 mRNA binding factors (PUF...step, researchers can now assemble designer PUF domains for RNA specificity engineering.
“The RNA binding...University of Illinois at Urbana-Champaign.
The PUF proteins include eight modular repeats, each of which binds...explained, the specificity of other RNA binding domains depends on the secondary structure of RNA. As a...eukaryotic RNA.”
Endless possibilities with PUF proteins
The tool she and her colleagues led by Zhao described...repressor. In principle, however, designer PUF proteins can be fused to other components to lend them ...