...the experts in the CRISPR/Cas field, have been extremely helpful for scientists that are looking for more...on “Making Your Own Competent Cells” has been extremely popular, generating many additional questions....and Money by Making Your Own Competent Cells By Melina Fan Plasmids 101: The Promoter Region – Let's...

...unmatched customer service that is consistently timely, informative, friendly, and professional Addgene..., Lianna, Luke, Marcy, Mary, Max, Maya, Meghan, Melina, Meron, Michael Lo., Michael Le., Michel, Michelle...Michelle, Morgan, Natashia, Nicole, Olivia, Pamela, Racheal, Ramsey, Randall, Ray, Rebecca, Renee, Shannon...

...adventure. Many thanks to our guest writer, Pamela J. Hines, PhD! Pamela J. Hines is a Senior Editor at Science...This post was contributed by guest writer Pamela J. Hines PhD, Senior Editor at Science Magazine. Although...

...This post was contributed by Manon Eckhardt and Melanie Brewer from the QBI Coronavirus Research Group ...to our guest bloggers Manon Eckhardt (left) and Melanie Brewer (right) from the University of California...California, San Francisco. Manon Eckhardt and Melanie Brewer are on the Science Communications Team at QBI UCSF...

...Commemorative Travel Awards: Melissa Chiasson, Elizabeth Unger, and Michal Bernach. Melissa Chiasson: Quantifying...one at a time is time consuming and unscalable.  Melissa Chiasson, a graduate student in Douglas Fowler'...

...factors (which repair those breaks) makes Cas9 extremely effective as a genome editing reagent. This interplay...2014) have shown that this technique supports extremely high frequencies of genome cutting and, when donor...DNA is included in the nucleofection reaction, extremely high frequencies of HDR. The detailed protocol...

...analysis performed in experiment 1. Use camelCase: In camelCase, each subsequent word in a file name after... more than one word should be named in the camelCaseFormat since spaces between each word can make it ...

...microbiome species such as Snodgrassella alvi and Gilliamella apicoli. The plasmids could also be transformed...antibiotic treatment. To facilitate these studies, Pamela Silver’s lab developed a synthetic mark and recapture.... PubMed Central PMCID: PMC6467691. Takahashi, Melissa K., et al. "A low-cost paper-based synthetic biology...

... questions, and (most importantly) decide on a timeline. My experience is that tasks often don’t get done...discussion can be confidential. Finally, provide timely feedback. We often associate feedback with end-...feedback should be given throughout the year and in a timely manner. If someone’s performance or behavior is...

...rings, dilution cloning into 96-well plates, is extremely time- and resource-consumptive, since only wells...densities for selection). A 2% agarose solution is then melted (and thereby sterilized) by microwaving and brought...left covered (to avoid drying out) while 3 ml of melted agarose are mixed with 3 ml of 2X DMEM in a 50 ...

...one developed for Zaire ebola virus (EBOV) are extremely useful, allowing researchers to study aspects ... systems, such as the one described here, are extremely useful research tools. In addition to the reduced... each plasmid transfected into cells are also extremely important. For example, particular ratios of plasmids...

...18 (end) - Panel Alex Tucker (Ginkgo Bioworks) Pamela Hines (Senior Editor at Science) Edward J. Hall...billions of dollars (Freedman et al 2015). Finally, as Pamela Hines of AAAS points out on the panel, irreproducibility...journalists and scientific experts discussed by Pamela Hines Minisymposium on Reproducibility Booklet ...

... of critical differences (adapted from Zhou and Melton, 2008, [43]): Characteristic Reprogramming+ ...induces expression of insulin genes in liver and ameliorates streptozotocin-induced hyperglycemia. Nat Med...32. PubMed PMID: 18754011. 43. Zhou, Q. and D.A. Melton, Extreme makeover: converting one cell into another...

...agent than BME and has a less… distinctive smell. BME smells like rotting eggs but has a longer shelf life...decision is probably already made: gradients are extremely difficult to handcast, so unless you have an expert...

...accelerate your research. Sincerely, Melina & The Addgene Team ... Melina Fan...

...project that would be a big deal if it worked out.”- Melina Fan, Chief Scientific Officer “Choose your PhD ...customized letter to the place that you are applying.” - Melina Fan, Chief Scientific Officer If there’s a career...

...cytochrome P450 genes discovered in Drosophila melanogaster (Gilbert, 2004). Mutations in these genes are...mediate steroid hormone biosynthesis in Drosophila melanogaster." Molecular and cellular endocrinology 215.1...

...help guide policies and ultimately treatments in a timely fashion.”  With this in mind, Bourque and Tara ...other research, scientific sharing will remain an extremely important part of the future of research. To find...

...you during the request stage, before you order.  Timeline Once your order is placed, we estimate it will...and QC assays to be completed and reviewed. The timeline therefore will be as follows:  Request review:...

...at one of my first “All Addgene” meetings, when Melina, our CSO and co-founder, approached me and asked... time for me to present, I thought I would be extremely nervous (I mean I WAS presenting to three department...