Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 227 results for: mel

Showing: 21 - 40 of 227 results

  1. Addgene’s Top 10 Blog Posts from 2014

    Type
    Blog Post
    Published
    Dec. 16, 2014, 8:01 p.m.
    ...the experts in the CRISPR/Cas field, have been extremely helpful for scientists that are looking for more...on “Making Your Own Competent Cells” has been extremely popular, generating many additional questions....and Money by Making Your Own Competent Cells By Melina Fan Plasmids 101: The Promoter Region – Let's...

  2. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    Published
    June 5, 2017, 3:19 p.m.
    ...unmatched customer service that is consistently timely, informative, friendly, and professional Addgene..., Lianna, Luke, Marcy, Mary, Max, Maya, Meghan, Melina, Meron, Michael Lo., Michael Le., Michel, Michelle...Michelle, Morgan, Natashia, Nicole, Olivia, Pamela, Racheal, Ramsey, Randall, Ray, Rebecca, Renee, Shannon...

  3. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    Published
    Sept. 26, 2017, 12:12 p.m.
    ...adventure. Many thanks to our guest writer, Pamela J. Hines, PhD! Pamela J. Hines is a Senior Editor at Science...This post was contributed by guest writer Pamela J. Hines PhD, Senior Editor at Science Magazine. Although...

  4. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    Published
    April 9, 2020, 1:15 p.m.
    ...This post was contributed by Manon Eckhardt and Melanie Brewer from the QBI Coronavirus Research Group ...to our guest bloggers Manon Eckhardt (left) and Melanie Brewer (right) from the University of California...California, San Francisco. Manon Eckhardt and Melanie Brewer are on the Science Communications Team at QBI UCSF...

  6. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    Published
    March 24, 2016, 2:30 p.m.
    ...factors (which repair those breaks) makes Cas9 extremely effective as a genome editing reagent. This interplay...2014) have shown that this technique supports extremely high frequencies of genome cutting and, when donor...DNA is included in the nucleofection reaction, extremely high frequencies of HDR. The detailed protocol...

  7. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    Published
    June 2, 2016, 2:30 p.m.
    ...analysis performed in experiment 1. Use camelCase: In camelCase, each subsequent word in a file name after... more than one word should be named in the camelCaseFormat since spaces between each word can make it ...

  8. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...microbiome species such as Snodgrassella alvi and Gilliamella apicoli. The plasmids could also be transformed...antibiotic treatment. To facilitate these studies, Pamela Silver’s lab developed a synthetic mark and recapture.... PubMed Central PMCID: PMC6467691. Takahashi, Melissa K., et al. "A low-cost paper-based synthetic biology...

  9. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    Published
    Jan. 30, 2018, 2:42 p.m.
    ... questions, and (most importantly) decide on a timeline. My experience is that tasks often don’t get done...discussion can be confidential. Finally, provide timely feedback. We often associate feedback with end-...feedback should be given throughout the year and in a timely manner. If someone’s performance or behavior is...

  10. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ...rings, dilution cloning into 96-well plates, is extremely time- and resource-consumptive, since only wells...densities for selection). A 2% agarose solution is then melted (and thereby sterilized) by microwaving and brought...left covered (to avoid drying out) while 3 ml of melted agarose are mixed with 3 ml of 2X DMEM in a 50 ...

  11. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    Published
    March 17, 2016, 2:30 p.m.
    ...one developed for Zaire ebola virus (EBOV) are extremely useful, allowing researchers to study aspects ... systems, such as the one described here, are extremely useful research tools. In addition to the reduced... each plasmid transfected into cells are also extremely important. For example, particular ratios of plasmids...

  12. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ...18 (end) - Panel Alex Tucker (Ginkgo Bioworks) Pamela Hines (Senior Editor at Science) Edward J. Hall...billions of dollars (Freedman et al 2015). Finally, as Pamela Hines of AAAS points out on the panel, irreproducibility...journalists and scientific experts discussed by Pamela Hines Minisymposium on Reproducibility Booklet ...

  14. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...agent than BME and has a less… distinctive smell. BME smells like rotting eggs but has a longer shelf life...decision is probably already made: gradients are extremely difficult to handcast, so unless you have an expert...

  17. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    Published
    Oct. 31, 2019, 1:06 p.m.
    ...cytochrome P450 genes discovered in Drosophila melanogaster (Gilbert, 2004). Mutations in these genes are...mediate steroid hormone biosynthesis in Drosophila melanogaster." Molecular and cellular endocrinology 215.1...

  18. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    Published
    Oct. 15, 2024, 1:15 p.m.
    ...you during the request stage, before you order.  Timeline Once your order is placed, we estimate it will...and QC assays to be completed and reviewed. The timeline therefore will be as follows:  Request review:...

  19. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    Published
    May 12, 2020, 1:15 p.m.
    ...help guide policies and ultimately treatments in a timely fashion.”  With this in mind, Bourque and Tara ...other research, scientific sharing will remain an extremely important part of the future of research. To find...

  20. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    Published
    June 29, 2023, 7 p.m.
    ...at one of my first “All Addgene” meetings, when Melina, our CSO and co-founder, approached me and asked... time for me to present, I thought I would be extremely nervous (I mean I WAS presenting to three department...
Showing: 21 - 40 of 227 results