We narrowed to 326 results for: nans

...Plasmid Collection, including tools using NanoLuc®, NanoBiT, and more. Which luciferase and assay have...blog Luminescent Imaging with Nano-lanterns  Technologies Enabled by NanoLuc® Luciferase Fluorescent Proteins... engineered a variety of improved luciferases. NanoLuc® Luciferase is the best known: it’s derived from...gracilirostris) Natural 455 19 Coelenterazine None Yes NanoLuc®(derived from Oplophorus gracilirostris) Engineered... (fluorescent and luminescent) worlds Forster Resonance Energy Transfer (FRET) is the transfer of energy...which then emits it as light. Bioluminescence Resonance Energy Transfer (BRET) works the same way, except...protein-protein interactions Split luciferases, such as NanoBiT, are expressed in two subunits and only catalyze...

...microenvironment’s malignancy but also incite different organs to become malignant. Exosomes also contain...on the conversion of non-malignant forms of cancer to invasive and malignant forms and noticed that myoepithelial...that when there’s less caveolin 1, there’s less malignancy because there’s less proliferation. However, ...exosomes are tools of communication and provide malignancy to the microenvironment itself. They deliver ...myoepithelial cells prevented progression to malignancy, concluding that these cells may be considered...PMC4788391. Kogure, Takayuki, et al. "Intercellular nanovesicle‐mediated microRNA transfer: A mechanism of environmental...Central PMCID: PMC3310362. Martins, Diana, and Fernando Schmitt. "Microenvironment in breast tumorigenesis...

...through the use of protein tags and separated from contaminants using a variety of purification methods. Deciding...expression systems are very powerful for high level recombinant protein expression. These systems enable high...cheap and low-tech means of mass expression of recombinant proteins. Many cells from various types of plants...modifications. Extraction and purification of recombinant proteins from plants however can be expensive...biochemicals and proteins through plant roots. Tagging recombinant proteins with a naturally secreted plant peptide...expression system to generate large quantities of recombinant eukaryotic proteins. Although many species of...using S. cerevisiae, researchers often place recombinant proteins under the control of the galactose inducible...

...serotype of the packaged DNA and look for potential contaminants. The analysis is a 2-step process using Geneious...total reads are most likely to correspond to the contaminant. Each contig is then BLASTed against the NCBI...contaminated. For AAV-68544, the best match for the contaminant is Addgene plasmid #44362. Method validation,... were easily able to identify AAV-44632, the contaminant we mixed into the original AAV sample at 5%. ...software looks for and quantifies the presence of recombinants in recombinase-dependent sequences. Recombinase-dependent...powerful tool that can be used to identify DNA contaminants in AAV preparations and provide information ...Encapsidation of Prokaryotic Sequences during Recombinant Adeno-Associated Virus Production and Their in...

...to identify the critical NANOG binding sites: 1. Find Enhancers that have NANOG binding sites 2. Design...ChIP-seq experiment (think “GPS for proteins”) for NANOG, an essential transcription factor in embryonic ... just over 13,000 significant binding peaks for NANOG in the human genome. But not all of those 13,000...Design a CRISPR screen to target and disrupt the NANOG enhancers 3. CRISPR out the enhancers from step 2. ... ESCs die or differentiate Step 3 reveals which NANOG-related genes are critical to stem cell survival...CRISPR in the next section. In order to denote the NANOG enhancers from step 1 with genome math, we’ll need...shorthand from the field of epigenomics: H3 – one of NANOG’s associated histone proteins K4 and K27 – locations...

... technology, but Oxford Nanopore is one of the most popular options. Nanopore sequencing deviates from...3: Overview of Nanopore sequencing technology. Created with BioRender.com. Nanopore can provide longer...sequencing methods (Figure 3). It uses (aptly named) “nanopores” that are attached to the flow cell membrane and...proteins. When each nucleotide passes through the nanopore, the electrical current recorded by the instrument...-read sequencing. At Addgene, we routinely use Nanopore technology to sequence our packaged viral DNA ...an entire viral genome in one read. We also use Nanopore sequencing to QC plasmids that are highly repetitive... Y., Bollas, A., Wang, Y., & Au, K. F. (2021). Nanopore sequencing technology, bioinformatics and applications...

... GFP-binding nanobodies, the project really took off. Unlike most antibodies, nanobodies, which occur ...inside cells. Nanobodies binding GFP were designed in 2009. Subsequent work showed that nanobodies could be...and money needed to develop new lines. Using nanobodies to create GFP scaffolds for transcriptional activation...  References 1.Tang, Jonathan C.Y., et al. “A nanobody-based system using fluorescent proteins as scaffolds...Modulation of protein properties in living cells using nanobodies.” Nat. Struct. Mol. Biol. 17 (2010): 133–138....Fluorescent fusion protein knockout mediated by anti-GFP nanobody.” Nat. Struct. Mol. Biol. 19 (2012), 117–121. ...

... Encoded Nanothermometer. Nano Lett. 2022 Jul 27;22(14):5698-5707. doi: 10.1021/acs.nanolett.2c00608. ... size (~1500 bp) makes iGECInano suitable for AAV packaging.    Find iGECInano plasmids here!   Matlashov....13.503850; doi: 10.1101/2022.08.13.503850.  iGECInano NIR calcium reporter Near-infrared (NIR) fluorescent...using small monomeric NIR fluorescent proteins (miRFP670nano and miRFP720) and the Ca2+-sensing domain from...from Twitch-2B (Matlashov et al. 2022). iGECInano displays better response kinetics and photochemical ...

...editing CHARM: A compact epigenetic silencer Recombinant antibodies for marking astrocytes MagIC-Cryo-EM... linkers and spacer proteins ending with a GFP nanobody (Figure 1). This enrichment streamlines the purification...alpha helix) and target capture module (SpyTag-GFP nanobody) assembled on beads. C) Micrograph of H1.8-GFP...spacers could enable many future variations using nanobodies or scFvs for other targets or could be used for...https://doi.org/10.1016/j.cell.2021.03.025.    Recombinant antibodies for marking cell types in the nervous...s Neuroscience Antibody Collection to find a recombinant anti-GFAP [N206A/8R] antibody shared by James...other cell types in the nervous system!  Find recombinant anti-GFAP [N206A/8R] antibody here!   ...

...Woolard, J., & Hill, S. J. (2021). Use of NanoBiT and NanoBRET to monitor fluorescent VEGF-A binding kinetics...HiBiT is a minimal 11-amino-acid fragment of the NanoBiT® complex, which, upon binding to its complementary... addition and a simple add-mix-measure method. NanoBiT® luciferase activity is compatible with both intra...LgBiT, and the authors used a p53 response-element NanoLuc reporter to show that the mutants abolished transcriptional...facilitating metastatic spread. The authors used NanoBiT assays to quantify ADAR1-HiBiT in cell lysates,...linking EV-mediated protein spread to enhanced malignant potential. This work highlights HiBiT tagging ...subtle changes in co-receptor dynamics, including NanoBRET assays to monitor the binding of fluorescently-labeled...

...confirmation of recombinant clones If a antibiotic resistance gene has been inserted, recombinants can first...outgrowth of bacteria. Selection and confirmation of recombinant clones.    Figure 2: Overview of using...replacements and tags.  The typical frequency of recombinants is 1 positive clone out of 104 to 105 colonies...easy to remove the recombineering system once recombinant clones are generated.  A simple way to do this...integrates, drug selection is not required for maintenance. A temperature shift to 42˚C not only allows ... Court DL. Enhanced levels of λ Red-mediated recombinants in mismatch repair mutants. Proceedings of the.... 2. Daiguan Yu, Hilary M. Ellis, E-Chiang Lee, Nancy A. Jenkins, Neal G. Copeland, and Donald L. Court...

...second day of Antibody, Addgene gave to me…  A recombinant anti-GFP!    🎶On the third day of Antibody, ...fourth day of Antibody, Addgene gave to me…  A recombinant Anti-Arl13b!    🎶On the fifth day of Antibody...sixth day of Antibody, Addgene gave to me… A recombinant Anti-PSD-95!    🎶On the seventh day of Antibody...eighth day of Antibody, Addgene gave to me…  A recombinant Anti-DYKDDDDK!   🎶On the ninth day of Antibody...tenth day of Antibody, Addgene gave to me…  A recombinant Anti-APP!   🎶On the eleventh day of Antibody... of Antibody, Addgene gave to me…  A growing recombinant antibody collection!     ...

...primate brains Endothelial-specific AAV delivery Recombinant anti-FLAG tag antibodies Peptide-assisted genome...throughout the non-human primate brain. Nature Nanotechnology, 18, 1241–1251 (2023). https://doi.org/10.1038....1038/s41467-023-38582-7. PMID: 37291094.   Recombinant anti-FLAG tag antibodies By Ashley Waldron Of...versatile. Whether you plan to purify or image a recombinant protein, the FLAG tag has proven itself a good...did you know that we distribute ready-to-use recombinant antibodies as well? Our collection includes a...available in trial sizes. Addgene recommends these recombinant antibodies for use in western blots, though the... variety of applications. Find anti-DYKDDDDK recombinant antibodies here!...

... of 1:1 (Zeltner et al., 2010). However, for recombinant AAV2, the same study reported a physical-to-infectious...2010), while another reported that only 20% of recombinant AAV2 particles were full, compared to 50% of ...David Bell and Svetla Stoilova-McPhie, Center for Nanoscale Systems, Harvard University.    Which titer...annealing can be affected by the presence of protein contaminants, which is a factor in AAV titration since the...packaging of genomes can limit production of recombinant AAV-2." Gene therapy 6.7 (1999). PubMed PMID:... Lock, Martin, et al. "Characterization of a recombinant adeno-associated virus type 2 Reference Standard...wild-type AAV as benchmark for infectivity of recombinant AAV vectors." Gene therapy 17.7 (2010): 872-879...

...nature can inform us on how to create better recombinant vectors. In this blog post, we’ll define plasmid...harness those features to create broad host range recombinant vectors.  A plasmid’s host range is determined...phylogenetic groups (Jain and Srivastava 2013).  Recombinant vectors that are made from broad host range plasmids...host-independent   Building broad host range recombinant vectors Replicons, which consist of a plasmid...broad host range plasmids can be used to create recombinant vectors that can replicate in different bacteria...to incorporating broad host range replicons, recombinant vectors can be modified further to increase the...cloning sites (Keen 1988).  Find broad host range recombinant vectors at Addgene!   References and resources...

...components into plants. Nanoparticles (carbon nanotubes, Kwak et al., 2019), DNA nanostructures (Zhang et al., ...chitosan-complexed single-walled carbon nanotube carriers. Nat Nanotechnol 14:447–455 . https://doi.org/10.1038...Hu P, Giraldo JP (2020) Targeted delivery of nanomaterials with chemical cargoes in plants enabled by a... Cunningham FJ, Fan C, Landry MP (2019) DNA nanostructures coordinate gene silencing in mature plants....

...expressed in frame on the N- or C-terminus of a recombinant protein of interest. The tag was developed as...wash steps removes excess proteins and other contaminants. The protein of interest is then eluted using...wash buffer should be high enough to disrupt contaminant-metal ion binding without eluting the target ...more stringent wash conditions and remove more contaminants without losing your protein of interest. But...Genetic Approach to Facilitate Purification of Recombinant Proteins with a Novel Metal Chelate Adsorbent...native histidine-tagged protein expressed by recombinant vaccinia virus. Proc Natl Acad Sci U S A. 1991...

...expressing the recombinant plasmid to propagate. Any cells that contained non-recombinant vectors (re-ligated...ccd operon, which is responsible for plasmid maintenance during cell division. ccdB codes for the toxic...the number of clones that do not contain the recombinant plasmid and therefore makes the cloning process... but should never be used when selecting for recombinant plasmids. Check out our blog post on Common Lab...References 1. Bernard, P. “Positive Selection of Recombinant DNA by CcdB.” Biotechniques. 1996 Aug;21(2):320...

...more complex and the presence of helper virus contaminants have slowed the use of this system. New and ...detailed instructions on how to generate the final recombinant adenoviral plasmid construct, please see Addgene... references 1, 2.*Pro-Tip*: once the correct recombinant pAdV plasmid is identified, re-transform into...2-3 weeks) - Here you’ll produce the primary recombinant adenoviral stock (rAdV-S). HEK293 cells are transfected..., et al. "A protocol for rapid generation of recombinant adenoviruses using the AdEasy system." Nature...2009): 547. PubMed PMID: 19373227. 4. Mittereder, Nanette, Keith L. March, and Bruce C. Trapnell. "Evaluation...

...escape in SARS-CoV-2 Recombinant antibodies for aminoacyl tRNA synthetases Nanocompartments for yeast bioproduction...Validated recombinant antibody toolbox for aminoacyl tRNA synthetases By Kate Harten Recombinant antibodies...Clouse Claudia Vickers’ lab has created a novel nanocompartment for transporting proteins in yeast by using...virus-like particles (MPyV VLPs ). This artificial nanoparticle can protect proteins from unfavorable interactions...