3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells
Type
Blog Post
Published
Sept. 5, 2017, 1:58 p.m.
...silent CRISPR/Cas9 blocking mutations in either the PAM (changing one of the G’s to an A,T or C – but avoid...improve editing efficiency.
But what’s better: PAM-blocking or guide RNA blocking mutations? This depends...silent. In our view, it’s always best to change the PAM site, however we have also demonstrated the efficiency...mutations. So if the locus does not allow blocking the PAM sequence (e.g. if it would lead to a missense or ...recommend changing several bases as close to the PAM as possible (see below to find out why). Please check...