CRISPR Guide
Type
Guide
...adjacent to a P rotospacer A djacent M otif (PAM). The PAM sequence serves as a binding signal for Cas9...found in the REC2, REC3, and PAM interacting domains and allows for expanded PAM recognition as well as increased... non-NGG PAM sequences . Another Cas9 alternative is xCas9 , which targets a broad set of PAM sequences...identifying all PAM sequences within the genetic region to be targeted. If there are no PAM sequences for...Cas enzymes ( see Cas9 variants and PAM sequences ). Once possible PAM sequences and putative target sites... near the PAM sequence, so gRNAs with no homology or those with mismatches close to the PAM sequence will...of Cas Enzymes Cas Enzymes and PAM Sequences Species/Variant of Cas9 PAM Sequence* Streptococcus pyogenes...