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We narrowed to 52 results for: pcas9

Showing: 21 - 40 of 52 results

  1. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    Published
    Dec. 12, 2017, 3:13 p.m.
    ...Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9 Day 10: Transcriptional regulation...The PAM Requirement and Expanding CRISPR Beyond SpCas9 Day 8: Repair mechanisms: CRISPR 101: Non-homologous...

  2. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ...Once upon a time, not so long ago, spCas9 was the only Cas enzyme widely available and applied by researchers...getting the desired features and cutting (note: spCas9 isn’t always the winner!).   Fig. 3 – Comparison...The PAM Requirement and Expanding CRISPR Beyond SpCas9 More resources from the Innovative Genomics Institute...

  3. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...the fact that it is smaller than the traditional SpCas9 enzyme (about a third of the size) and does not...cleave RNA through the same mechanism as traditional spCas9. If you are looking to modify RNA, Cas9 just won...The PAM requirement and expanding CRISPR beyond spCas9 Cas proteins More resources on the Addgene Website...

  4. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...Yoon, S., & Kim, H. H. (2019). SpCas9 activity prediction by DeepSpCas9, a deep learning–based model with... and PE5 prime editing strategies) based on a DeepSpCas9 score ranking (Kim et al., 2019). PRIDICT saves...

  5. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...CRISPR, and specifically Cas9 from S. pyogenes (SpCas9), is truly an exceptional genome engineering tool... species, and has many applications. That said, SpCas9 is not the only game in town, and other Cas proteins... can circumvent the limitations associated with SpCas9. Cas13a (previously referred to as C2c2), has several...

  6. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...period of time. High fidelity Cas9s such as eSpCas9 and SpCas9-HF should be explored to reduce off-target...mutation in dystrophin exon 23. Long et al. used SpCas9 in conjunction with AAV9, whereas Nelson et al....

  7. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...capacity of a locus. When using S. pyogenes Cas9 (SpCas9), potential target sites are both [5’-20nt-NGG]...editing, there are usually few potential gRNAs. While SpCas9, with a PAM preference of NGG, is still the most...gRNAs that are likely to be active, not only for SpCas9 but also for some other Cas enzymes. It seems to...

  8. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...used for each gene. At the moment, 13 full genome SpCas9 knockout libraries designed by different academic...Library, for example related to strategies beyond SpCas9, or species not currently included in Green Listed...libraries. As of now I have focused on human and mouse SpCas9 knockout libraries, but there are of course great...

  9. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    Published
    Dec. 6, 2016, 3:30 p.m.
    ... makes it unuseable. With the large size of the SpCas9 nuclease (~4.2 kb) this is a particularly prominent...Institute to provide lentiviruses for delivery of SpCas9 and Cas9 nickase for genome editing. You can also...

  10. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...crRNAtracrRNACas9 crRNACpf1 Endonuclease size spCas9: ~4 kbsaCas9: ~3 kb ~3.9kb crRNA/gRNA length...sequence, tRNAs, multiple individual promoters).  spCas9 and its gRNAs are also larger than their Cpf1 counterparts...

  11. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ...protein The MLV Gag protein fused to a Flag-tagged SpCas9 is incorporated into Nanoblades, just like the ...these variants are fused to the MLV Gag in place of SpCas9. gRNA A gRNA targeting a DNA sequence of interest...

  12. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    ...well characterized than the conventionally used SpCas9 (derived from the bacterium Streptococcus pyogenes...been shown to exhibit more limited activity than SpCas9 (2). In many cases this is due both to lower relative...

  14. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    Published
    May 8, 2018, 1 p.m.
    ...multiplex CRISPR-Cas9 double-strand DNA breaks using SpCas9. More features will be added to the ICE software...configured for Cas9 fromStreptococcus pyogenes (SpCas9). ICE Score - The editing efficiency (percentage...

  15. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ... for reduced off target editing, such as SpCas9-HF1, eSpCas9(1.1), or HypaCas9. Read more about Cas9 options...

  16. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ... deaminase portion of the base editor to create SpCas9 base editors with editing windows as small as 1...available fused to ABEmax(7.10) or to BE4max   Evolved SpCas9 with NRN or NYN PAM site Chen et al., 2023 Td-CBE...

  17. Editor's Choice, July 2016

    Type
    Blog Post
    Published
    Aug. 5, 2016, 3 p.m.
    ...compared to the most commonly used Cas9 variant, SpCas9. These properties, combined with other benefits...

  18. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ...create a colorful Cas9 toolbox, Ma et al. turned to SpCas9 and its orthologs NmCas9 and St1Cas9. Each ortholog...

  19. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ...editing in moderate thermophiles.  These evolved SpCas9 variants have improved activity on NAG PAMS and...
Showing: 21 - 40 of 52 results