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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,494 results for: pcas9

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Showing: 1 - 20 of 2494 results

  1. pAAV-EFS-SpCas9 (AAV8)

    Viral Prep
    #104588-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-EFS-SpCas9 (#104588). In addition to the viral particles, you will also receive purified pAAV-EFS-SpCas9 plasmid DNA. EFS-driven expression of Myc-tagged SpCas9. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ryohei Yasuda
    Article
    Nishiyama et al Neuron. 2017 Oct 18. pii: S0896-6273(17)
    Promoter
    EFS
    Available Since
    Jan. 14, 2026
    Availability
    Academic Institutions and Nonprofits only

  2. pAAV-RSV-SpCas9 (AAV8)

    Viral Prep
    #85450-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-RSV-SpCas9 (#85450). In addition to the viral particles, you will also receive purified pAAV-RSV-SpCas9 plasmid DNA. RSV-driven expression of SpCas9. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Hetian Lei
    Article
    Duan et al J Biol Chem. 2016 May 31. pii: jbc.M116.
    Promoter
    pRSV
    Available Since
    Jan. 14, 2026
    Availability
    Academic Institutions and Nonprofits only

  5. pCas9

    Plasmid
    #167547
    Purpose
    Used for CRISPR-Cas9 mediated recombineering in Enterococcus. Can clone desired gRNA using BsaI digestion.
    Depositor
    Howard Hang
    Article
    Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00
    Inserts
    chloramphenicol acetyl transferase
    pUC19 origin of replication
    Use
    CRISPR
    Expression
    Bacterial
    Available Since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    167547-plasmid-map-sequence-id-332733

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  7. Nickase SpCas9 - pCMV-T7-nSpCas9(H840A)-P2A-GFP (HES140)

    Plasmid
    #208976
    Purpose
    Nickase SpCas9 (H840A) with a P2A GFP and 3xFLAG, expressed from a CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    nSpCas9(H840)-BPNLS-P2A-GFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A)
    Promoter
    CMV and T7
    Available Since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    208976-plasmid-map-sequence-id-415837

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  13. pCas9(D10A)

    Plasmid
    #74495
    Purpose
    Nicking Cas9 derived from pWT Cas9 (Addgene #44250). Ampicillin resistance marker, the tetracycline repressor (TetR), a ColE1 origin of replication and Streptococcus pyogenes Cas9 D10A
    Depositor
    Xiao Wang
    Article
    Standage-Beier et al ACS Synth Biol. 2015 Nov 20;4(11):1217-2
    Insert
    Cas9 D10A
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    D10A
    Promoter
    pTeto
    Available Since
    May 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    74495-plasmid-map-sequence-id-140495

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Showing: 1 - 20 of 2494 results