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We narrowed to 50 results for: phage

Showing: 21 - 40 of 50 results
  1. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ...packaged into phage and then extracted. Besides taking a couple days to generate the phage-derived uracil...with mutational scanning: ligand binding assays, phage display assays, ubiquitination activity assays, ...
  2. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...transcription of the phage repressor cI. Repressor cI inhibits transcription from phage promoter pR, preventing...
  3. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...editors By Susanna Stroik The Liu lab recently used phage-assisted continuous evolution and protein engineering... here!  Doman, J.L., Pandey, S., et al. (2023). Phage-assisted evolution and protein engineering yield...
  4. What's New in CRISPR - September 2019

    Type
    Blog Post
    Published
    Sept. 10, 2019, 12:56 p.m.
    ...CRISPRi screens to identify essential genes and phage host factors, and identify sequence-specific toxicity...
  5. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...single array to target the genome of the invading phage pathogen. Over the past decade, CRISPR tools have...
  6. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ... to maximize the mutation rate. For example the Phage Assisted Continuous Evolution (PACE) method requires...
  7. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...occur naturally in any known genome other than P1 phage, and is long enough that there is virtually no chance...
  8. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...transcriptase domain for optimization. They developed phage-assisted evolution strategies to evolve more effective... Tolar, J., Osborn, M. J., & Liu, D. R. (2023). Phage-assisted evolution and protein engineering yield...
  9. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...2018). xCas9 was generated by the Liu lab through phage-assisted evolution, specifically with the goal of...
Showing: 21 - 40 of 50 results