We narrowed to 51 results for: phage

Data-driven design of Golden Gate Assembly overhangs greatly increases the number of fragments that can be used in cloning.

Aug. 1, 2023, 1:15 p.m.

One-pot saturation mutagenesis can quickly generate libraries of mutant plasmids for phenotypic screening. Learn more from SciComm intern Beth Kenkel.

Feb. 22, 2017, 3:30 p.m.

Some promoters are only active under specific circumstances. Here's a guide to inducible promoters, which are regulated by chemicals, light, and/or temperature.

Jan. 18, 2018, 2:34 p.m.

Learn all about how to use a new plasmid for RNAi in C. elegans, tools for proximity labeling, RNA-based methods for detecting viral outbreaks, and tools for characterizing protein domain binding specificities.

Dec. 6, 2018, 1:34 p.m.

This month's Hot Plasmids articles features new plasmids, new antibodies, and a viral ticker tape system!

Nov. 14, 2023, 3:35 a.m.

Learn about CRISPR plasmids at Addgene such as an E. coli genome-wide CRISPRi library, GeneWeld vectors, CasX plasmids, and drug inducible CRISPR/Cas9 activation.

Sept. 10, 2019, 12:56 p.m.

This post describes applications of pooled libraries, briefly explains several approaches, and highlights the most popular pooled libraries at Addgene.

Nov. 19, 2025, 2:15 p.m.

Learn how the 2017 Addgene-Seeding Labs Plasmid Grant Awardees will be using plasmids in their research.

July 18, 2017, 12:41 p.m.

The Addgene viral team has found a way to increase AAV yields through media supplementation of sucrose, an endocytosis inhibitor.

Dec. 13, 2018, 1:14 p.m.

Drive-and-proccess (DAP) arrays are a new tool from the Gao lab that allows for up to 31-loci CRISPR multiple base editing.

July 7, 2022, 1:15 p.m.

Targeted mutagenesis helps molecular biologists make changes to a DNA sequence at a precise location. Read on to learn more about EvolvR, a CRISPR-Cas9 based targeted mutagenesis method from the Dueber Lab.

Feb. 21, 2019, 1:15 p.m.

Learn how DNA methylation may affect the activity of certain restriction endonucleases.

June 30, 2016, 2:30 p.m.

Overview of Cre-lox recombination and how it is used to make DNA modifications in transgenic animals, embryonic stem cells, and tissue-specific cell types.

Jan. 13, 2015, 3:47 p.m.

This post discusses takeaways from, and tools presented at, the Deaminet 2026 conference, focusing on base editors, prime editors, and translational work.

March 10, 2026, 1:14 p.m.

Learn all about new plasmids available at Addgene including an anti-CRISPR optogenetic tool, the fluorescent voltage indicator Archon1, photoswitchable proteins, and a simplified 2in1 cloning method.

March 19, 2019, 1:31 p.m.

A review of alternative PAM and PAM-flexible Cas enzymes.

March 7, 2024, 2:39 p.m.

Plasmid-encoded antibodies offers many advantages over other types of antibodies. Learn about how recombinant antibodies are made.

May 4, 2021, 1:15 p.m.

This round-up of new plasmids from Addgene include blue-light inducible Cre for use in bacteria, CRISPR-Casɸ mGold, and new CRISPR plasmids and viral preps.

Jan. 5, 2021, 2:15 p.m.

Joanne Kamens provides highlights from the 2015 Canton Nucleic Acids Forum (CNAF).

Dec. 1, 2015, 3:30 p.m.

Read on for a sampling of some of the exciting new plasmid technologies that have come through our doors in 2015.

Dec. 23, 2015, 3:30 p.m.