Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 72 results for: phage

Showing: 21 - 40 of 72 results

  1. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ...packaged into phage and then extracted. Besides taking a couple days to generate the phage-derived uracil...with mutational scanning: ligand binding assays, phage display assays, ubiquitination activity assays, ...template is generated by transforming a backbone phagemid vector into an E. coli strain that is deficient...

  2. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...transcription of the phage repressor cI. Repressor cI inhibits transcription from phage promoter pR, preventing...

  4. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...editors By Susanna Stroik The Liu lab recently used phage-assisted continuous evolution and protein engineering... here!  Doman, J.L., Pandey, S., et al. (2023). Phage-assisted evolution and protein engineering yield...

  5. What's New in CRISPR - September 2019

    Type
    Blog Post
    Published
    Sept. 10, 2019, 12:56 p.m.
    ...CRISPRi screens to identify essential genes and phage host factors, and identify sequence-specific toxicity...

  8. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...single array to target the genome of the invading phage pathogen. Over the past decade, CRISPR tools have...

  9. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ... to maximize the mutation rate. For example the Phage Assisted Continuous Evolution (PACE) method requires...of the thioredoxin-binding domain (TBD) from bacteriophage T7 DNA polymerase increased the processivity...

  12. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...occur naturally in any known genome other than P1 phage, and is long enough that there is virtually no chance...from the P1 bacteriophage: the Cre recombinase and a loxP recognition site. The P1 bacteriophage uses these...Let us know in the comments! References 1. Bacteriophage P1 site-specific recombination. I. Recombination...in mammalian cells by the Cre recombinase of bacteriophage P1. Sauer, B. and Henderson, N. 1988. PubMed...

  13. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...transcriptase domain for optimization. They developed phage-assisted evolution strategies to evolve more effective... Tolar, J., Osborn, M. J., & Liu, D. R. (2023). Phage-assisted evolution and protein engineering yield...

  15. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...hypercompact genome editor from the Biggiephage clade of huge phages. In this article, they investigated...as in human and plant cells. Casɸ from Biggiephage can eliminate competing mobile genetic elements...

  16. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...2018). xCas9 was generated by the Liu lab through phage-assisted evolution, specifically with the goal of...

  19. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...by using 6-12 phage PP7 coat protein binding sites and 24 copies of the MS2 bacteriophage coat protein ...Synthetic Biology Center have deposited a set of 11 phage integrases which act irreversibly on their cognate.... These pairs are closely related to the lambda phage integrase system which is the basis for Gateway ...authors recently used computational protein design, phage display and high throughput binding assays, to engineer...

  20. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...Central PMCID: PMC3760253. 2. Rohwer, Forest. "Global phage diversity." Cell 113.2 (2003): 141. PubMed PMID:...more abundant are the Earth’s estimated 1031 bacteriophages (viruses that infect bacteria), many of which...
Showing: 21 - 40 of 72 results