...this time using the Staphylococcus aureus Cas9 (SaCas9). This AAV-based genome editing system is regulated... in Molecular Neuroscience Find the AAV-based SaCas9 genome editing plasmids String assembly gRNA ...

...electroporation. Then at your preferred time points, sacrifice the animals and fix the samples. When you use ...AAV titer for the best results. The timing of sacrifice when using Tetbow AAVs - You will have to wait...

... tested, including SpCas9, SaCas9, LbCas12a, enAsCas12a, SpCas9-NG, SaCas9-KKH, CP1028-SpCas9, and CP1041...

...primarily pertains to ORIs in budding yeast, Saccharomyces cerevisiae; however, we’ve also noted some features...required for the replication of fission yeast, Schizosaccharomyces pombe, vectors at the end.) In a previous...

...biochemistry department at the University of Massachusetts Medical School. We are working with postdocs...to hold. There are institutions in Boston - Massachusetts General Hospital, Mass Eye and Ear Infirmary...

...by Kivanc Birsoy. Find the library here A new SaCas9 ABE variant (microABEI744) has improved on-target...footprint compared to current N-terminal linked SaCas9 ABE variants. It's also one of the smallest AAV-deliverable...

...CRISPR. AAV transduction Only compatible with SaCas9 (packaging limit ~4.5kb). CRISPR elements are inserted...AAV particles. Transient or stable expression of SaCas9 and/or gRNA. Infects dividing and non-dividing ...

...under the control of an inducer, i.e. tet-off transactivator (tTA), is injected at a high dose to maximize...An AAV expressing the inducer (i.e. tet-off transactivator) is co-injected. The dose of this AAV can be...

...by Kusumika (Kushi) Mukherjee, a Postdoc at Massachussetts General Hospital. A little over a decade back...Mukherjee is currently a postdoctoral fellow at Massachusetts General Hospital with research interests in ...

...et al 2017 use a dual vector system composed of SaCas9 and a gRNA in one AAV and a repair template in ... by the fact that even small Cas9 homologs like SaCas9 and their requisite gRNAs take up essentially all...

... of the fly (Pfeiffer et al., 2010). Yeast (Saccharomyces cerevisiae) Yeast, one of the simplest eukaryotic....5 (2011): 190-195. Liu, Wei, et al. "From Saccharomyces cerevisiae to human: The important gene co‑expression...

...pharmaceutical industry, depend on the yeast, Saccharomyces cerevisiae, as a key host for the production... of dCas9-VPR plasmids, which already includes dSaCas9-VPR in an AAV transfer vector for in-vivo expression...designed to complement unused auxotrophies in Saccharomyces strains by compensating for histidine (HIS3)...

...photoswitchable spCas9, dCas9, and S. aureus Cas9 (saCas9) proteins, showing the versatility of this approach...This study generated the first optically active saCas9 protein. Stop light! Photoactive anti-CRISPRs ...

...Features 85169  pJL-SaBE3  SaCas9 (NNGRRT)    85170  pJL-SaKKH-BE3  SaCas9 (NNGRRT)    85171  pBK-VQR-BE3...

...travel to Addgene's headquarters in Watertown, Massachusetts, for the occasion! Addgenies enjoying the ...

...this kit to complement unused auxotrophies in Saccharomyces strains.  Listen to Our Segment on The Yeast...

...Alex Chavez, a Clinical Fellow in Pathology at Masachusetts General Hospital and Postdoctoral Fellow in ...

...finally December, and here at Addgene HQ in Massachusetts that means it’s time for near-freezing temperatures...

... chilly this winter as the Addgenies are in Massachusetts, have a look at the Dyer Lab’s decorated office...

...be entirely endotoxin free. Endotoxin is a lipopolysaccharide of the cell wall of Gram negative bacteria...