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... Replogle, J. M., Adriaens, C., Ramadoss, G. N., Shi, Q., Hung, K. L., Samelson, A. J., … & Weissman, ...plasmids for MagIC-Cryo-EM here!   Arimura, Y., Konishi, H.A., Funabiki, H. (2024) MagIC-Cryo-EM: Structural...

.../genetics.115.176594 Yang H, Wang H, Shivalila CS, Cheng AW, Shi L, Jaenisch R (2013) One-Step Generation... find that many, if not all, the mice carry a frameshift mutation. For knock in with a donor oligonucleotide... genome. However, with only 3.4% of founders establishing germline transmission of the transgene, this...https://doi.org/10.1038/nbt.3026 Gurumurthy CB, Takahashi G, Wada K, Miura H, Sato M, Ohtsuka M (2016) GONAD...doi.org/10.1038/s41467-017-02706-7 Wang H, Yang H, Shivalila CS, Dawlaty MM, Cheng AW, Zhang F, Jaenisch R ...

..., Liu, J., Zhang, H., Xie, L., Ying, W., Li, D., Shi, L., Sun, Y., Li, Y., Li, Y., Sun, Y., & Zuo, E. ...

... 2011. 767: p. 411-8. PubMed PMID: 21822892. 33. Shi, Y., P. Kirwan, and F.J. Livesey, Directed differentiation...thanks to our guest blogger, Kusumika (Kushi) Mukherjee. Kusumika (Kushi) Mukherjee is the Editor of Trends...This post was contributed by Kusumika (Kushi) Mukherjee. The ultimate goal in the field of regenerative... pursue a career in science communication and publishing after completing her postdoctoral training from...441(7097): p. 1061-7. PubMed PMID: 16810240. 2. Takahashi, K., et al., Induction of pluripotent stem cells... 131(5): p. 861-72. PubMed PMID: 18035408. 3. Takahashi, K. and S. Yamanaka, Induction of pluripotent ...

....2013.04.025  Yang, H., Wang, H., Shivalila, C. S., Cheng, A. W., Shi, L., & Jaenisch, R. (2013). One-Step...10.1016/j.cell.2011.06.019  Wang, H., Yang, H., Shivalila, C. S., Dawlaty, M. M., Cheng, A. W., Zhang, F...

...collaborations and partnerships. There are many different ways to maintain a relationship - many of them ...about the current state of a partnership. Every collaboration and partnership is different as are the individuals... the next level. External collaborations and partnerships are also very important in a non-research context...although we do that too), we will often look for partnerships where we can leverage another organization’s...’s expertise to our mutual benefit.  Our partnership with SnapGene, for example, allows us to display ...and now must focus on managing the established partnership. If you’re willing to do similar things for your...position at Addgene I am now involved in Strategic Partnership Management, a very interesting and exciting part...

...residence or citizenship to continue their work in the US have the added burden of needing sponsorship. If you...create therapeutics to cure the cancer. Authorship:  Publishing books, journals online and/or in print.... in the United States. There has been a growing shift away from employers sponsoring individuals for green... permanent residence case with United States Citizenship and Immigration Services (USCIS). If it were ...last year less than 30% of applicants received sponsorship) then he or she can start working in October ...can be a good choice for individuals needing sponsorship. Academic H1B The academic H-1B allows employers...National Interest Waiver (NIW) allows for self-sponsorship of the I-140 application meaning that the category...

... a variety of science policy roles including fellowships, volunteer positions, and part-time opportunities... give advice and guidance to those that ask. Relationship building is key in science policy. Luckily, ...happy hours that you can attend to help build relationships.  Reach out to a science policy professional... product or a well-endowed philanthropy who can shift the funding landscape to focus on a neglected disease...the organization, these changes to policy could shift how society interacts with certain scientific areas...compiled by the AAAS Science and Technology Policy Fellowship Program to learn more about where science policy...policy? Many science policy training programs and fellowships have a strong focus on early career researchers...

...urged you to work on developing your “leadership skills”. Leadership is prized at all levels of an organization...All three roles are important to accomplishing any goal or finishing any project. Doers are the ones who...Business Review 10 Must Reads: On Leadership (Articles on Leadership, also can be purchased individually...inundated with articles and books on the topic of leadership. Perhaps one of your advisors or mentors has ...   I am often asked to give career seminars on Leadership Skills. After attempting to put together such...what skills were really leader-specific. How is leadership different from good management? Aren't all career... doers, some become managers and some take on leadership roles. You may be happy with a primary role of...

... voltage will shift the population to the right while decreasing the voltage will shift it to the left... peak that shifts to the right on a histogram plot and lower for a signal peak that shifts to the left...103, while the GFP+ population cluster would be shifted to the right in comparison, say somewhere between...underwent different treatment processes, you may see a shift in the negative population, so it no longer overlaps...shows peaks of high signal densities, so signal shifts due to upregulation or downregulation are easier... plots For smeary expressions, you can quantify shifts in signal using the mean fluorescence intensity... the green (top) has an upregulated expression (shift to the right). The blue (third from top) could be...

...platforms.  As you can imagine, shipping to so many countries means that our shipping team is always engaged in... country that we can export to — so far, we’ve shipped materials from our repository to an amazing 111...contracted with so we can always offer the fastest shipping. However, there were issues that persisted despite...Table 2: Addgene distributors Distributor Partnership Start Date Country Served Beijing Zhongyuan...materials, we noticed a large percentage of these shipments arrived in India at room temperature. Partnering...distributor for that country. In China, direct shipments weren’t able to clear import control. Partnering...number of barriers. Though we haven’t solved every shipping issue for every country, we’re continuing to use...

...mentor. How do you make the most of this new relationship? Consider adding formality and active goal setting... setting to your mentoring relationships, so that you can reap rewards in the form of reaching career ... career path is a great start to a mentoring relationship, but if you don’t take this past the chatting...meetings and setting the direction in a mentoring relationship falls on the shoulders of the Mentee. A Mentor...progress over the course of an ongoing mentoring relationship (in a book or online). Mentee: Make a list of...secret of accountability To make a mentoring relationship really productive, the most important step you... PhDs with interesting careers, find a summer internship, etc.). It is helpful to work together on setting...

...amongst the lab team, shipping team, and others to ensure that everything will ship out in time - from assembling...tubes, and to packing and shipping. Every single day. 673 plasmids shipped daily may be easy to picture...milestone by ending the day with 1,000,002 plasmids shipped. Reaching one million plasmids shared might have...picture (see the picture of our shipping room above), but as Joanne Kamens, Addgene’s executive director,...celebration was so much fun! Here's the Addgene Leadership team in all their photo booth glory! #1millionplasmids... and we just reached our 10,000th viral vector shipped! In the upcoming months, we will be moving into...

... then be recognized by a DNA-binding molecule (Hoshino and Fujii, 2009). Other forms of enChIP, that do...29533181. PubMed Central PMCID: PMC5849414. Fujita, Toshitsugu, and Hodaka Fujii. "Efficient isolation of specific...2013): 132-136. PubMed PMID: 23942116. Fujita, Toshitsugu, et al. "Identification of telomere-associated...24201379. PubMed Central PMCID: PMC3821016. Fujita, Toshitsugu, et al. "Identification of non-coding RNAs associated...25874893. PubMed Central PMCID: PMC4395285. Fujita, Toshitsugu, Miyuki Yuno, and Hodaka Fujii. "Efficient sequence‐specific...2016): 370-377. PubMed PMID: 26848818. Fujita, Toshitsugu, et al. "Identification of physical interactions...2017): 506-520. PubMed PMID: 28474362. Fujita, Toshitsugu, Miyuki Yuno, and Hodaka Fujii. "An enChIP system...

...planning you can do to build a bench mentor-mentee relationship and bring your best self to the training experience...plenty of articles on building successful mentor relationships, asking for mentor support, and choosing your...group, you will also typically receive technical mentorship from a ‘bench mentor’. In some instances, the...the research group). The bench mentor-mentee relationship is extremely important – without a good set ...mentor than your scientific mentor. Thus, this relationship will probably define your experience in the ...Luckily, you can do a lot to contribute to the relationship dynamic and set yourself up for success! Getting...information in your training. Bench mentor-mentee relationships Training can be a very fulfilling experience...

... career development. Publishing Panel The Early Career Researchers in Publishing Panel brought in editors...academia Require that postdoc salaries be paid on fellowships instead of grants such that PIs no longer save...Path Panel and the Early Career Researchers in Publishing panel, focused on showing researchers that they... PIs and all gave advice on how to succeed in publishing in the current academic environment. Some great...writing skills will help you in your academic publishing goals and make you a better communicator overall...have any questions or concerns throughout the publishing process. The diversity panel - Equity and inclusion...the work of underrepresented groups under their mentorship with the same critical eye they place on others...

... to diverse audiences Organization Mentorship Leadership Science writing competitions (e.g.... Consulting internships Communication Teamwork Management Leadership Networking ...the clear answer with a well-defined path to professorship. But recently a downward trend in funding and...the lab highlight “transferable” skills such as leadership, management, and communication. A great approach... Publicity Business competitions   Leadership Management Marketing Communication Business...Conference or club organizer Management Leadership Communication Networking As the number...

... build the relationship in a non-awkward way?  7 ways to strengthen your new relationships Here are some...Relationships need nurturing. Perhaps you’ve met someone interesting at an event. Or, perhaps you have...contacts you have; it’s about building genuine relationships with people you care about knowing. How do you...some ways to go about strengthening a new relationship with authenticity and not discomfort: Invite the...meeting (in person or virtually) will make the relationship stronger.  If appropriate, invite them to speak...same people is often the easiest way to build relationships and strengthen them over time.  Things to ...important” or senior people. Focus on building relationships with peers and people around you naturally. ...

...illuminating-transmembrane-proteins-using-hibit-crispr-cell-lines/ Shibata, C., Otsuka, M., Shimizu, T., Seimiya, T., Kishikawa, T., Aoki, T., & Fujishiro, M. (2023...exosomal transport is quite challenging. Work by Shibata et al. (2023) used HiBiT to measure the activity... conditioned media, and purified EV fractions (Shibata et al., 2023). Luminescence signal was used to ...VEGF-A to the heteromeric receptor complex. Find sHiBiT-KDR Fusion Vector at Addgene! HiBiT advances detection... https://doi.org/10.1021/cb3002478 Kamada, Y., Tateishi, H., Nakayamada, U., Hinata, D., Iwasaki, A., ...Hirosuna, K., Ozato, T., Yamamoto, H., Hirasawa, A., Ennishi, D., Tomida, S., Toyooka, S., & Otsuka, M. (2025...

...you're going to commit to doing a postdoctoral fellowship, what are you signing up for and how can you ...briefly describe some tips for a successful postdoc fellowship. 1. Reverse engineer success On Day 1 of your...preparation) A history of successful funding (fellowship grants) A project with sufficient preliminary...need to be there? A typical biomedical postdoc fellowship lasts five years. Most universities and institutes...publications, you will be more competitive for fellowships. If you explore your system more, you will have...favorable impressions on potential manuscript or fellowship reviewers, expose your research to search committees...write manuscript drafts, reviews, cover letters, fellowships, etc. Second, get constructive feedback from ...