Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 286 results for: tac

Showing: 21 - 40 of 286 results

  1. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ...of the manager is not to empower but to remove obstacles.” – Scott Adams, Dilbert cartoonist What makes...for example. So aside from a focus on removing obstacles, what makes a good manager? First, it takes an...their present positions By removing the primary obstacle of job dissatisfaction, the work can get done,...blogs in this series will address more specific tactics managers can utilize to be in touch with the needs...

  2. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    Published
    Aug. 31, 2023, 1 p.m.
    ...named NeuroStack was used (Orguc et al., 2020). Using a programmable microcontroller, NeuroStack coordinates...rechargeable battery, is relatively small, and can be detached easily whenever necessary. This is all an impressive...tissue. They performed optical stimulation using NeuroStack and the fibers as previously described, then ...

  3. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ...this post for a refresher on AAV titers Making contact with a cell Although an individual AAV particle...to infect a cell, it has to come into physical contact with that cell. Since there is a limited amount... area on each cell, the way to get more AAV in contact with the cell is to increase the AAV concentration...expression It’s clear that we need high titers, as contact between the cell and any individual AAV particle...

  4. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type
    Blog Post
    Published
    June 25, 2019, 12:54 p.m.
    ... or sex pilus that attaches to the recipient cell drawing it close. Once in contact the donor can transfer...viral vector. One example are bacteriophages that attach to bacterial membranes and inject their genetic...bacterium to a recipient bacterium through direct contact. The donor bacterium contains a DNA sequence called...

  5. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ...biology-related customer support interaction. Before contacting customer service In an age of Tweeting and texting...customer service experience: Make sure you’re contacting the correct company. This might seem like a no-brainer...questions. BUT, I do believe that scientists who contact customer service deserve prompt, courteous responses...responses. No one is likely to look forward to contacting customer service, but the experience should never...

  6. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ...up your poster, and pack a few business cards to tack up next to your poster. Arrive early: You might ...well ahead of time ensures you aren’t scrambling to tack up your poster seconds before the session begins...popular posters at the end of the session. Get in contact See a poster you want to know more about? Interested...related to your research? Find a way to get in contact with the presenter after the session. Exchange ...

  7. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...proteolysis-targeting chimera (PROTAC) was developed for HaloTag. PROTACs are engineered molecules which...self-cleave and the protease and degron remain attached to the tagged protein. In this event, the degron...it is a straightforward system – HaloTag and its PROTAC are all that is needed to facilitate degradation...

  8. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ...Despite these grim statistics, new strategies for attacking cancer are yielding promising results in the laboratory...universally-dividing behavior of cancer cells, attacking the cellular machinery necessary for division....technologies become available almost daily, yet obstacles remain. One of the biggest challenges facing researchers...Pathway v2.0 Page Resources on the Addgene Blog Tackling Cancers' Drug Resistance with a New Screening ...

  10. Career Insights: Technical Support Specialist

    Type
    Blog Post
    Published
    Nov. 9, 2017, 2 p.m.
    ...absolutely convinced it was the company’s fault. I contacted them to express my concerns and request a replacement...customer support conversation, just go ahead and contact a technical support team with a question or two... for asking a quick question or making initial contact, but is less suitable for troubleshooting or issuing... friendly, patient, attentive, empathetic, and tactful. Occasionally it can also be necessary to take ...

  11. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ...advocacy will be required. Information on how to contact your representatives in case you would like to ... them about science: Representatives: To find contact information for your representative in the House...need to know is your area code. Senators: To find contact information for your senators all you need to do...state. State Legislatures: To find the website to contact representatives at the state level you can click...

  12. Congrats to the 2019 Deck The Lab Winners

    Type
    Blog Post
    Published
    Jan. 17, 2020, 3 p.m.
    ...tape garland. Thanks @ribosholmes!   Frogs and tacos The Miller Lab at the University of Michigan decorated... sweaters and Santa hats. There was even a giant taco... Miller lab annual #DeckTheLab. Getting in the...frog is coming to town. @Addgene @UMich_MCDB #christmastaco #frogsnowflake #xenopus #ASCBEMBO19 #MCDB 🎄...

  13. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ...greater than 75 plasmids, please let us know by contacting [email protected]. These deposits are considered...us at  [email protected] Special cases Please contact us at [email protected] or +1 (617)-ADDGENE (...deposit a pooled DNA library with Addgene?" or contact us at [email protected] or +1 (617)-ADDGENE (...genomic alterations unique to your strain. Please contact us at [email protected] or +1 (617)-ADDGENE (...

  14. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    Published
    Jan. 2, 2014, 3:44 p.m.
    ...the lab earlier, to read that stack of papers on your desk, and to tackle some new experiments before the...from webpage clippings, photographs, and file attachments. Organize your notes into notebooks, create checklists...

  15. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    Published
    Nov. 22, 2016, 3:30 p.m.
    ... Many thanks to our guest blogger Sean Stacey! Sean D. Stacey is a Ph.D. student at East Tennessee State...This post was contributed by guest blogger Sean Stacey who recently attended both online and on site courses...

  16. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    Published
    Sept. 15, 2015, 2:30 p.m.
    ...is no coming back. The whole lab is emotionally attached to this disease.” He says that the award from ...Patients affected by AHC suffer from recurrent attacks of unilateral paralysis (hemiplegia) that are often...often painful and accompanied by migraines. Those attacks can be brought on by a number of stressors, including...

  17. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ... Many thanks to our guest blogger Sean Stacey! Sean D. Stacey is a Ph.D. student at East Tennessee State...This post was contributed by guest blogger Sean D. Stacey. Opinions on the apps discussed in the article ...

  18. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    ...unsuccessful cloning reaction leaves the α-peptide intact, and therefore the cell will have a functional ...whitish-cream color of standard E. coli. On the other hand, intact β-galactosidase produces pigment from x-gal (included...important to give your plates enough time for any intact β-galactosidase to be expressed and process x-gal...

  19. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    Published
    July 22, 2014, 5:46 p.m.
    ...conversation about GMOs.  Challenge #2: Technical obstacles to plant synthetic biology In addition to public...understanding of plant biology is a significant technical obstacle to plant synthetic biology. We cannot build what...color-changing flower, and we’d love to hear about the obstacles you’ve faced as a plant scientist, and how you...

  20. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    Published
    Feb. 4, 2014, 3:50 p.m.
    ...Building Relationships for Success”. Choose 1-2 tactics to try. One of my favorites is making the pledge...which relationships will take off, or who your new contact will connect you to. Sometimes, it is not who you...searchable databases are usually offering their contact information so you can tap them for advice. As ...
Showing: 21 - 40 of 286 results