...usual quality and success. Customer and technical support Our customer and technical service brands...helpful and the customer and technical service teams put 100% effort into helping our customers every day in...always adding to our “google doc of gratitude.” We must keep spreading the word about materials sharing,...scientific fields.” Thanks to our legal team, and great customer service, somehow we have scientists actually loving...spreading the word. Our blog and site resources are just killing it for scientists. Our website is visited...request from us but also by high school students and industry scientists as a universal resource for information...offer online for Addgenies and to serve external customers. I also can’t wait to see where our new data initiatives...

...optogenetics toolbox with CRY2clust Article contributed by Brook Pyhtila  Listen to the CRY2clust podcast segment...peptide. CRY2clust should prove a useful tool to further probe cellular function. The CRY2clust Plasmid...developed another useful optogenetic tool that enables robust and efficient oligomerization of target proteins...proteins in response to blue light. This tool, CRY2clust, was created by adding a 9-residue peptide to the ...CRY2, although some studies have shown that this clustering only happens under certain conditions. With...With its additional 9 amino acid peptide, the CRY2clust domain enables fast and reversible oligomerization...reversible upon termination of light stimulation. CRY2clust enables faster assembly and disassembly dynamics...

...-science skills is important for success Must always justify use of resources and pay attention to budget...training progresses.  In From Academic Solos to Industrial Symphonies, an article well worth reading on ...can directly interfere with a seamless jump into industry.” In his article On Leaving Academe, Terran Lane...budget constraints Academic science is much like industry but the “product” is publications—the pressure... is the same You get to interact with scientists just like you What is different about academia? Publishing... with the academic calendar Academic researchers must be extreme self-starters and especially good at ...publications are a nice to have, not a need to have Must be ready to change projects frequently with little...

...a specific question. Optogenetics is a tool and, just like any other tool in a scientist’s vast toolbox...proven. Therefore, a concrete, well defined, and robust behavioral output is sufficient to assess the effectiveness...opsin) are also available. Picking your opsin  You must consider whether you wish to stimulate or inhibit...recombinase while still taking advantage of the robust expression via the promoter in the AAV construct...neuroscience research technique often used beyond just optogenetics. Successful stereotaxic surgery relies...inject virus into 20 or more mice and the injections must be near identical for all of these surgeries. Identify...injection prior to using the virus. You may need to adjust coordinates based on your own method of identifying...

...explained that the data must be reproducible by someone else. Second, "an entrepreneur must be OK with the constant...quickly realize that a company requires more than just good data to become a product. I spoke with Dr. ... the best, wins. He explained that entrepreneurs must clearly communicate their idea at various levels...is. While communication styles in academia and industry vary drastically, he reassures that one can learn... the team was unable to secure enough money to sustain a startup, he went to work for Flagship Ventures...energy and effort into making the startup self-sustainable. Within a year from this decision, Mike and his...that are aligned in vision and outcomes. The team must work well together, have complementary skill sets...

...likely to see once a year. There was lots of hustle and bustle, but the lab was very competitive and not ...decisions are based on emotions and assumptions. I’m not just talking about simple things like “Which route should...identify your priorities and needs, but sometimes it just takes introspective thought on what’s worked for...interact regularly with the newer lab members. You must get an idea of what to expect and decide if it is...outside of academia 10 years after your training. You must ensure that you are expanding your options and preparing...(topic and/or technical aspects) applicable to industry if this path interests you  (e.g. human disease...drug/device development? Does the advisor have industry connections such as serving on Boards? Does the...

...processes, distribution, and customer service) Addgene provides unmatched customer service that is consistently...scientists accomplish their goals. Many nonprofits must spend a lot of resources continuously raising money... revenue to build reserves so the company is sustainable. The difference is what nonprofits do with their...materials are affordable but enough to make us self-sustaining. With this unique model, we are able to spend...that scientists and technology transfer offices trust us to work for them with full transparency. We don...purposes and not for a profit motive. We build this trust by keeping excellent records and maintaining confidentiality...specifically drives the decision making process at Addgene, just look to our core values. These accurately reflect...

...it doesn't look done, just keep working on it. Also bear in mind that this is just a review article and...topic when I set out to write the review. That’s just to say that you don’t have to be one either. Choose...wasn’t an expert in the field. To find reviews, I just searched online and found ones that I thought “looked... looked for papers from a wide range of authors. Just start writing When I first started I thought I would...a normal day.” The way I broke this cycle was to just start writing. A word of advice: when reading, don...summarized results and contextualized them. No really, just start writing! Don’t worry about grammar or formatting...like you don’t still know enough about the topic. Just get as many words down on the page as possible. ...

...A manager must both hire and fire to make sure the team is the best it can be. Managers must ensure that...although they must occasionally). Good leaders exert influence in other ways. While good managers must motivate... skills leaderhsip skills when done well? Do you just have to know it when you see it? “The Three Stages...they sometimes dig in and do some of the work, they must be more focused on delegating tasks and in motivating...management style. Leaders look to the future Leaders must get new things started and then step back to let...  Fire, Snowball, Mask: How Leaders Spark and Sustain Change, by Peter Fuda and Richard Badham Leadership...Goldsmith, with Mark Reiter Harvard Business Review 10 Must Reads: On Leadership (Articles on Leadership, also...

...and then you adjust. But that’s what keeps things interesting. And hopefully they’ll be just as interesting...pandemic. The Pandemic Pivot Back in March 2020, just four months after I started with Addgene, I was ...a protocol that didn’t require any lab shooting, just animation. The plan was for her to shoot everything...detail as possible when showing lab methods. So, this just wasn’t going to work for the majority of our educational...camera and audio, and the on-screen talent could just focus on their interview or performance. By this...rephrase questions was key. I had also hoped that just having me there would ease people enough.  Build...multiple interview subjects, I positioned a boom mic just out of frame allowing people to sit without having...

...primary role is customer support, but there are eight other teams who still retain a customer support element...disagreements. In fact, conflict resolution isn’t just about resolving conflicts between other people, ...conflict can lead to people disagreeing with each other just for the sake of disagreeing. It can be extremely...that a conflict is personal (”Person A and Person B just don’t get along”) when in fact many sources of conflict...through multiple channels is not reasonable or sustainable. That said, when you know that a person or team...assume that they saw what they needed to see and just didn’t have anything to contribute. Also keep an...other responsibilities. Knowing which aspects of customer support each team “owns” is critical for day-to-day...

... include: 1) Lost trust Perhaps the greatest risk is that the public will lose trust in scientists if ...useful to governments, however, inconvenient data must not be ignored. Returning to an earlier analogy,...scientist and politician Rush Holt said, government must have the same fundamental attribute that enables...science: “humility in the face of evidence” (4). Frustration among scientists has been rising as evidence ...lab and march. Unintended consequences While frustration and anxiety have inspired people to rally for... supporting unwanted results (14). Some loss of trust, I fear, has already come to pass. It may only be...detrimental for them to be homogenized or thought of as just another party. Additionally, interest groups are...

...make them serve you well. The happy byproduct might just be a career opportunity. The focus of this article...Talk 2. Tailor every talk to the occasion Don’t just plan to reuse a prepared talk unless it perfectly... narrative. Scientists do this all the time, you just need to figure out how to make it suspenseful. 4...t worry if your work hasn’t been commercialized, just talking about the possibility is interesting. Check...determine how long the talk will really take and to adjust content and slides to better suit the target time...details on visuals in science presentations. There is just no excuse for poor quality slides with sufficient...answer to, but if you are, never make up an answer. Just say you will get back to them with an answer, and...

... only modestly improve muscle function, and they must be injected regularly. Dystrophin and genome editing... therapy to be successful, multiple requirements must be met. First, CRISPR would need to be delivered...much work is still needed. First, CRISPR delivery must be optimized to: 1. Reach a high percentage of muscle...in muscle, as well as unwanted germline editing, must be rigorously examined over a long period of time...editing over time, but this approach would require robust stem cell editing to maintain the desired phenotype...mutations. In addition to upregulating HDR, NHEJ must be downregulated to prevent the introduction of ... each therapy, effectiveness and off-target risk must be evaluated separately, increasing the time to ...

...social life and rest. I need it just as much in my current role as Customer Support and Operations Manager...they are transferrable across most, if not all, industries and work environments. This is why they are called...another interview, and this job was made for you. They must have been looking at your resume while they wrote...times the hiring team will also want to examine not just technical skills, but also transferable skills, ...takes to thrive at the company and add value, not just “do the job”. When they ask about your creativity...Might you already have leadership skills that are just not being tapped at the moment? You can take this...develop your nascent leadership skills. You might just find that you’re more of a leader than you thought...

...concept called multiplexing. Since CRISPR is such a robust system, editing or labeling efficiency doesn’t ...processing a continuous multi-gRNA transcript, each gRNA must be expressed from its own promoter. But that doesn...your chosen gRNA target sequences and clone them in just as you would for a single gRNA. You’ll clone in ...creating flanking overhangs. These overhangs can be customized to link together multiple fragments, allowing...destination vector that expresses Cas9. This is illustrated in the schematic below.   Figure 2: ...1x4; for 6 gRNAs, you’d use pX330A-1x6. This customization means you don’t ever need to clone in filler...plasmids allow expression of 2-5 gRNAs in zebrafish. Custom destination vectors are used depending upon the...

...Toolkit Many industries, such as the food and beverage industry, the bioethanol industry, and the pharmaceutical...for genetic optimization of industrial yeast strains. However, industrial yeast strains are more difficult... necessary constructs, and complete screening in just 3 days? Thanks to Tyler Jacks’ lab, this 3-day goal...yeast cells. Named after the brightly-coloured Australian parrot, the dual colour-emission biosensor Rosella... a new method for generating protein chains or clusters. Veggiani, et al. Proc Natl Acad Sci U S A. 2016...pharmaceutical industry, depend on the yeast, Saccharomyces cerevisiae, as a key host for the production...transform. To increase the genetic toolbox for industrial yeast strains, the Borodina Lab has recently ...

...lead to frustration. Clinging to technical tasksDelegation can feel unnatural for those accustomed to hands-on... PhDs Making the Leap to Industry." He successfully transitioned to industry after his PhD and a couple... PhDs face when transitioning from academia to industry is whether to stay hands-on in the lab or pivot...exploring alternative career trajectories within the industry, but the transition wasn’t easy - I had to reinvent...adapt to these differences could have led to frustration, micromanagement, and disengagement. By tailoring...witnessed several colleagues do this while changing industries and positions. Some people discover they truly...

...also adjusted voltage and compensated your colors. Here are some good practice tips on adjusting voltage...with adjusted voltage, cells that did not receive the GFP protein are most likely to be clustered at signals...plots discussed in this post, voltage has been adjusted appropriately and compensation performed. Before...not be clearly picked up by the sensors. You can adjust this by changing the voltage for the particular... signals below 103, while the GFP+ population cluster would be shifted to the right in comparison, say...gating populations of interest with normal gates, just based on a histogram plot instead of a dot plot....

... it out. 4:00 PM--Respond to customer help tickets. Catch up on customer help tickets, and respond when...advocate for the customer and help build software and technology tools with the customer in mind. You can...Oxford Nanopore Technologies, I work with our customers in the US to learn where the gaps are in their... so many different people! I communicate with customers, software developers, as well as team members ... It’s divided into the key takeaway themes and customer pain points we’ve identified. I can’t wait to ...know what to work on next to help alleviate any customer pain points. Many thanks to our guest blogger...