...and feel the satisfaction of checking off each step as you painstakingly complete your experiment – one...Addgene Website: http://www.addgene.org/ If you’re working with plasmids and you decide to share them using...of useful molecular cloning protocols, including making LB agar plates, restriction digest of plasmid DNA.../ Labguru is a good place to go if your lab is looking to streamline your lab management and you want ...reagent storage and inventory, managing orders, tracking students and research projects, electronic lab...

...modulating the production of the pro-inflammatory cytokine interleukin IL-1 beta in the dorsal raphe nucleus (DRN... circular corridor apparatus using a camera or tracking software. Locomotion is a highly complex process...reflect elements of drug addiction and craving (‘seeking drugs’).  What’s involved in locomotor sensitization...protected) rather than being out in the open (risky). Taking advantage of these characteristic traits, rodents...reduced anxiety levels and a higher tendency for risk taking. These animals also show signs of neuroinflammation...

...consider a dual-sgRNA system. This system uses a “nicking” Cas9, in which one of Cas9’s two critical enzymatic...deleting the catalytic residue of a protein or knocking out a very small peptide. In these cases, you ...xCas9 has a PAM requirement of NG, GAA, or GAT, making it a more flexible cutter with the added bonus ... You can achieve your precise edit without even making a break.  Base editors are dead Cas9 (dCas9) or...sites was sufficient to repress transcription by blocking its initiation. In mammalian cells, robust repression...interest. dCas9-based repressors are useful when knocking out a particular gene isn’t feasible. For example...

...chat is a convenient alternative for asking a quick question or making initial contact, but is less suitable...to look forward to in a technical support role Talking to customers everyday, it can be fascinating and...right place to live it out, but ideally without asking too many (annoying) questions. Identifying and ...understanding of when to use these communication skills by working with people from many different backgrounds and... other roles in industry. Many thanks to Eric Perkins and Stephanie Hays for images used in this post...

..., 2020.   Scientists around the world have been making major improvements to CRISPR technology since its...blue light is readily absorbed and scattered by the skin, so it is not able to reach deeper tissues. Additionally... however, can penetrate 5 mm past the surface of skin and is less phototoxic. Using the FAST system, Ye...By fusing the two pdDronpa domains to the sites flanking the DNA-binding cleft of Cas9, the fluorescent...turn on transcription, label chromatin, and study kinetics. Using chemistry to photocage CRISPRs The aforementioned...spatial control of Cas9 cleavage when using a photomasking technique. Last, Hemphill et al. presented data...techniques (Matony et al., 2020). Whether you are looking to activate, repress, or modify a gene, you now...

...model organism in scientific research. Yes, the same kind we use in breads and other baked goods! Yeast is... the study of neurological diseases, such as Parkinson’s disease (PD). PD is primarily characterized by...C. elegans are also self-fertile hermaphrodites making it easy to conduct large experiments over multiple...60% of human genes have an ortholog in C. elegans making it ideal to study basic molecular biology processes...resources check out Addgene’s Worm Expression page. Looking for more model organisms? Check out Part two in...From the baker to the bedside: yeast models of Parkinson's disease." Microbial cell 2.8 (2015): 262. PubMed...

...politics? Science is an apolitical process for seeking knowledge. The process begins with a testable hypothesis...experiments can help support or reject a hypothesis. Looking at the data, scientists then revise their hypotheses...scientists who hold a doctorate degree, are currently working full-time, and have received a research grant in... of the new administration’s actions, including taking down the climate change page on whitehouse.gov,...depending on the number of people supporting it.  3) Linking scientists to one particular political party while...politicians and citizens to include science in decision making, it will be considered a success. Below are resources...

...without restructuring. Positive Points for Trainees - Taking Steps to Achieve a Successful Career Career Advice...various career opportunities through our career, networking, and management for scientists blog series. We...Session focused on finding this information and making it more easily digestible/available. What I viewed...welfare of the many researchers out there who are making great progress on some of the world’s most profound...At Addgene we hope that all parties involved in making academic research a successful and enjoyable path...Blog Post Read Our Career Blog Posts Read Our Networking Blog Posts ...

...delighted to see enterprising early career scientists taking an active part in creating their own opportunities...opportunities for career development and networking. Aside from the importance of the topics to be discussed, ...building valuable skills and meeting new people by working together on the event planning and execution. I...seeing many similar examples of science trainees taking a proactive role in their non-bench training. Grad...all. For grad students and postdocs interested in taking matters into their own hands, there are some easy...

...honing your science communications skills and networking. And on social media, you could be sharing your...Science Communications and Marketing Coordinator Networking “Many, perhaps most of the most interesting jobs...have to create opportunities. If you find yourself asking, "Why doesn't this product/organization/position...position and my team at Addgene was created.”- Eric Perkins, Director of Science Product Management “I knew.... Usually when I see inspiring artists who are thinking about getting into the arts (or any career to ...