... a protospacer flanking sequence, so you can target virtually any RNA sequence! In bacteria, Cas13d-mediated...The Cas13d proteins they identified have little sequence similarity to previously identified Cas13a-c orthologs...

...working on lab operations software for next-gen sequencing (NGS), and high-throughput screening. While some...in the US to learn where the gaps are in their sequencing workflows, any problems they’re having with our...region. She is particularly interested in DNA sequencing tech, and the intersection of technology, software...

...in your organism or cell type? You can use DNA sequencing or other molecular cloning techniques to determine...resistance gene followed by the target site (the same sequence you’d like to mutate in the genome) and an out-of-frame.... A gRNA that targets your desired gene (whose sequence is also found in the reporter gene). A control...

...: shRNAs are short RNA sequences which target complementary mRNA sequence for degradation. Viral introduction...viral vector for your gene of interest – an shRNA sequence specific to your gene’s mRNA or a sgRNA targeting...

...reconstituted in vivo to form a protein identical in sequence to the unmodified enzyme. Each half of the base... cell. Intein splitting removes any exogenous sequences and regenerates a native peptide bond at the split...markers, transformation acceptor vectors, and sequences for genomic integration by homology directed repair...

...These vectors contain an Autonomously Replicating Sequence (ARS) derived from the yeast chromosome. As the...part of an ARS along with part of a centromere sequence (CEN). These vectors replicate as though they ...DNA (apparently independent of a known specific sequence) dictate the replication of these vectors. S. ...

...HHCS-associated sequences had lower IREB2 binding enrichment compared to wild-type sequences and that loss...however, knock-in of BoxB sites into endogenous DNA sequences could allow one to study RNAs expressed from their...

...lowering Cas9 expression, and truncating gRNA sequences used for targeting; however, these options can...the gRNA (see figure below) Because off-target sequences have less complementarity to the gRNA, they have...Lab similarly thought that if they could weaken sequence independent interactions between Cas9 and DNA,...

... available, and the collection of samples and sequencing is being organized and funded by someone else...the makerspace comfortable with next-generation sequencing data and the bioinformatics pipeline. It’s possible... the Illumina GAIIx we recently acquired  for sequencing again - it’s too inefficient and the reagents...

...also the first eukaryotic genome to be entirely sequenced and is very amenable to genetic manipulation. ...organisms, the C.elegans genome is also completely sequenced and over 60% of human genes have an ortholog in...Kerstin, et al. "The zebrafish reference genome sequence and its relationship to the human genome." Nature496.7446...extrachromosomal maintenance and integration of transforming sequences." The EMBO journal 10.12 (1991): 3959-3970. PubMed...