We narrowed to 798 results for: KIN

... STED nanoscopy Article contributed by Eric J. Perkins Listen to Endosomal pH Sensors podcast segment ...approach allows minute-by-minute analysis of the kinetics of chromatin regulation and subsequent mathematical...

...proteins in your assay.  Narrowing the field After working your way through the above steps, you may find ...an antibody website. In 2016, the International Working Group for Antibody Validation proposed 5 pillars...

...developed by engineering the Cas12f scaffold gRNA and making strategic mutations in the DNA binding cavity of...half the size of the Cas9 or Cas12a based systems, making it well suited for AAV packaging. Most importantly...

...colleagues are working on developing new surface display vectors to improve expression and trafficking of these...

...unpublished results at a conference? Post your working manuscript as a preprint too and you can share ...American Society for Cell Biology in a position working with preprints in life sciences. He also posted...

...out there, it’s obvious that CRISPR is a ground-breaking technology that’s already had a huge impact on...nuclease itself. Design and testing of eSpCas9 Looking at the structure of the Cas9 nuclease (PDB ID: ...

...something that is actually useful to you. When embarking on a new literature search, try to find a recent... on in any given research area. Unless you are looking for a particular protocol or are trying to replicate...

... been working with the Rare Genomics Institute to provide plasmid grants to researchers working on rare...

...or their translation disrupted through physical blocking of the translation machinery. In the Sánchez lab...planarians. Depending on which planarian species one is working with, the protocol will slightly differ. For example...

...fluorescent protein Dronpa. The authors tracked protein kinase A (PKA) activity at the plasma membrane in living...artifacts, it has better folding and maturation kinetics than other dark fluorescent proteins, it has good...

... to own the process entirely remains a large undertaking. Priority number one was to ensure that this ... load it on to our new MiSeq and set up the run making sure to note the appropriate barcode for each sample...

...specific gene or brain region. When it comes to making the genetic manipulation, there are two main decisions...should be used? Modifying gene expression Generally speaking, one may want to achieve either inhibition or ...

...engineered mice: transgenic mice, knockin and knockout mice, and conditional knockin or knockout mice. While these...

... ability to study and understand the complex interworking of the 86 billion neurons in the human brain... opens the door to in vivo experiments such as tracking the synaptic maturation pattern of hippocampal...

...detection We and other members of the lab had been working on advancing the paper-based platform toward a ...when we needed additional expertise. Perhaps most striking is that there were seven institutions involved...

...has slightly different activation/inactivation kinetics. For example, the light-gated cation channel, ...protein-coupled vertebrate rhodopsin in cerebellar Purkinje cells. The Journal of biological chemistry. 2011...

...setup. This requires a less involved setup when working with mice and slightly less red tape for approval...has psuedotypes. Psuedotyping is the process of making a hybrid AAV, with the genome from one serotype...

... fluorescent proteins.  Currently, the team is working to identify how MagLOV’s structure changes under...starting point for much more. If you’re interested in working on these questions or collaborating, the York Lab...

...bacteria can grow and compete in the same container, making it easier for a smaller, recombined plasmid to ...developing your amplification protocol! We recommend looking at protocols used for libraries that are similar...

...binding distinct PAM sequences. Now, Hu et al., working in David Liu’s lab, have gone one step further,...Once you’ve tried it, let us know how xCas9 is working for you in the comments section! References Hu...

...and could be useful to labs wishing to create this kind of animal models. Start using adenoviral vectors...and also Adeno Cas9 and Adeno EA. Or if you're looking for a broader range of CRISPRs plasmid tools, find...

... side, successful indel generation and epitope knockin was achieved by a few respondents suggesting that...inconvenient. Another possible reason for the trickiness of NgAgo is the inability of mature NgAgo protein...

...more CRISPR tools are being published each month, making CRISPR a great choice for your next experiment!...ties directly back to your specific goal. When thinking about step 3, however, you may be surprised at...

...absorbance strength is low compared to other reagents, making it more difficult to detect. A particularly strong...applications and that you use the same solution for blanking your machine!  Finally, many NanoDrop systems ...

...culture experience. Let’s go over the basics of making rAbs. Production Recombinant antibody production... because it secretes fewer of its own proteins, making the purification process simpler. Due to its high...

...Ampicillin, often just called amp, is the historical king of the antibiotic resistance realm. Amp is a semisynthetic...period after transformation (the dreaded 60 minutes shaking at 37 degrees) can be shortened since the plated...

...don’t require a PAM sequence at the target locus, making them more flexible than Cas9/Cpf1. Some Cas13 enzymes...prefer targets with a given single base protospacer flanking site (PFS) sequence, but orthologs like LwaCas13a...

... are likely to order several plasmids at once, breaking the statistical independence assumption of the... biology.       David R. Liu is Director of the Merkin Institute and Vice-Chair of the Faculty at the ...

... bonds with water molecules and coaxes it into sticking to the hydrophobic glass filter. Each glass filter...depending on the size of bacterial culture you’re working with. Overall, the glass syringe filter method ...

...the transformation efficiency of the strain: when asking the cell to perform multiple recombination events...recommend Phusion or Q5. Speed up your life Rather than making up your PCR mix every time, pre-made aliquots of...