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We narrowed to 786 results for: KIN

Showing: 571 - 600 of 786 results

  1. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ... ability to study and understand the complex interworking of the 86 billion neurons in the human brain... opens the door to in vivo experiments such as tracking the synaptic maturation pattern of hippocampal...

  7. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ... to own the process entirely remains a large undertaking. Priority number one was to ensure that this ... load it on to our new MiSeq and set up the run making sure to note the appropriate barcode for each sample...

  8. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...detection We and other members of the lab had been working on advancing the paper-based platform toward a ...when we needed additional expertise. Perhaps most striking is that there were seven institutions involved...

  9. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    Published
    Sept. 17, 2015, 2:30 p.m.
    ...has slightly different activation/inactivation kinetics. For example, the light-gated cation channel, ...protein-coupled vertebrate rhodopsin in cerebellar Purkinje cells. The Journal of biological chemistry. 2011...

  11. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ...binding distinct PAM sequences. Now, Hu et al., working in David Liu’s lab, have gone one step further,...Once you’ve tried it, let us know how xCas9 is working for you in the comments section! References Hu...

  12. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...setup. This requires a less involved setup when working with mice and slightly less red tape for approval...has psuedotypes. Psuedotyping is the process of making a hybrid AAV, with the genome from one serotype...

  13. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ... fluorescent proteins.  Currently, the team is working to identify how MagLOV’s structure changes under...starting point for much more. If you’re interested in working on these questions or collaborating, the York Lab...

  14. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ...bacteria can grow and compete in the same container, making it easier for a smaller, recombined plasmid to ...developing your amplification protocol! We recommend looking at protocols used for libraries that are similar...

  15. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    Published
    Aug. 4, 2016, 2:30 p.m.
    ... side, successful indel generation and epitope knockin was achieved by a few respondents suggesting that...inconvenient. Another possible reason for the trickiness of NgAgo is the inability of mature NgAgo protein...

  16. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    Published
    Sept. 24, 2015, 2:30 p.m.
    ...more CRISPR tools are being published each month, making CRISPR a great choice for your next experiment!...ties directly back to your specific goal. When thinking about step 3, however, you may be surprised at...

  17. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...don’t require a PAM sequence at the target locus, making them more flexible than Cas9/Cpf1. Some Cas13 enzymes...prefer targets with a given single base protospacer flanking site (PFS) sequence, but orthologs like LwaCas13a...

  19. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...475nm Lyn-tag (derived from the tyrosine-protein kinase Lyn) Membranes 3 mCherry 587nm/ 610 nm human ...Anderson JC, Dueber JE, Leguia M, Wu GC, Goler JA, Arkin AP, et al. BglBricks: A flexible standard for biological...

  20. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...culture experience. Let’s go over the basics of making rAbs. Production Recombinant antibody production... because it secretes fewer of its own proteins, making the purification process simpler. Due to its high...

  21. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    ...Ampicillin, often just called amp, is the historical king of the antibiotic resistance realm. Amp is a semisynthetic...period after transformation (the dreaded 60 minutes shaking at 37 degrees) can be shortened since the plated...

  22. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ... bonds with water molecules and coaxes it into sticking to the hydrophobic glass filter. Each glass filter...depending on the size of bacterial culture you’re working with. Overall, the glass syringe filter method ...

  23. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...the transformation efficiency of the strain: when asking the cell to perform multiple recombination events...recommend Phusion or Q5. Speed up your life Rather than making up your PCR mix every time, pre-made aliquots of...

  25. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ... insert-specific sequence. This is a “yes or no” kind of test, with a positive clone amplifying a product...this type of primer pair anneals to a sequence flanking the insert and one primer anneals to the insert...

  26. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...and public health and medical organizations are working to help educate the public about proper antibiotic...observe any mutations at the target site. In a mouse skin colonization model, phagemid treatment decreased...

  27. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...library screen only to find the system itself is not working because your reporter protein doesn’t function ...Rajagopala S.V., Stellberger T., Uetz P.  Exhaustive Benchmarking of the yeast two-hybrid system. Nature Methods...

  28. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    Published
    Aug. 20, 2015, 2:30 p.m.
    ...and develop an area of expertise. Whether you’re seeking a full-time job or want to be an independent grant...a mentor, find your people Find someone who is working or has worked as a Grant Professional to mentor...

  29. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...optogeneic actuators and sensors at Addgene. Cell marking/selection: Expression constructs like plasmids ...Because of its stability, GFP can be used in lineage tracking capacities in cell fate studies. It can also be...

  30. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...many questions from scientists all over the world looking for some additional information or clarification...functional before investing time and resources into making a stable cell line. When expressing a viral construct...
Showing: 571 - 600 of 786 results