We narrowed to 888 results for: URE

...selection-based gene tagging approach Multiple features of C. elegans have made CRISPR/Cas9 so successful... sequences are inserted into the genome. Figure 1: Self-excising cassette schematic from Dickinson...efficient for C. elegans genome modification. Figure 2: Schematic from Dickinson et al. (2015) illustrating...may prove effective in other species or in cell culture. In addition to CRISPR/Cas9 applications, SEC selection...

... possible for added sensitivity. As reported in Nature Methods, the FRET changes of these Twitch sensor...by a secondary screen in rat hippocampal neuron cultures. The optimized Twitch sensors allowed the researchers...toxic,” Merkx explained. New tools were needed to measure them. Merkx says, while in principle adapting a...them,” Merkx said. “For us, it is one of the main measures of success – whether we have really developed ...

...use agreements like MTAs.  Addgene does a lot to ensure that we can facilitate a fast and agile MTA process...material under the specified terms. This acts as the signature on any subsequent MTA for that plasmid facilitated... handled the majority of our MTA requests and signatures via an online process, though the process has...materials are requested, instead of coordinating signatures from two different institutes. Our online portal...

...participants could share their notes together. Figure 1: Can you draw Spongebob from these instructions...visuals, or asking questions using the polling feature or the chat box. One key thing to remember regardless...getting to know the app or virtual platform and making sure you stay connected. If possible, test out the technology...connection,” says Abitua about her workshop. “We made sure that we had a backup plan in case someone’s internet...

..., analyzed your data, and made your tables and figures. Now it's time to write. Sometimes, it's hard to...Linux.  Mendeley and Zotero have a lot of the same features, but this chart highlights the differences between...Can Grad School podcast episode 2 "Organizing Literature" : I found out about Zotero here Dear Grad Student...blog: Tips for writing scientific manuscripts: literature searching, reading, and organizing Tips for writing...

...serotonergic neurons (measured using a red calcium sensor) leads to the release of serotonin (measured using the ...throughput manner. She helped develop a method that measures the effects of sequence variants on abundance ...metabolism of leaves can be changed by light and temperature and leaf autofluorescence can interfere with ...

...actually mean? What relevance does it have on the structure of a bacteria species and how it can be used in...differences in cell wall composition. Cell wall structure Gram positive bacteria, or monoderms, have a single...the gram negative bacteria’s thinner membrane. Procedure The stain is a pretty simple process. First, flood... them red or pink. At the end of the staining procedure, gram positive bacteria are stained purple or ...

...CRISPR screening.   Using tRNAs Luckily, a fun feature of tRNA was fully unleashed by the Gao Lab’s work... for gene editing. They named this new array architecture the DAP array, short for “drive-and-process....payloads. Different from existing multiplex array architectures, including previous tRNA-gRNA arrays, that rely...Comparison of previous representative dual-gRNA architectures with DAP array   Qichen Yuan is a PhD...

...course (read our blog post on it and to see old pictures of the lab!). By 2016, we wanted to be taken seriously...working in our laboratory! Don’t worry though, we make sure that everyone gets proper equipment and safety training...project at BosLab. Once he optimizes plant cell culture and transgenesis he wants to share his knowledge...yeast transformations! Dennis Oleksyuk, Raphael Laurenceau, and Tami Pudina have initiated a soil microbiome...

...problem or complete a project, overcoming inevitable failures and setbacks. There are many roles that actively...because they enjoy spending time outdoors and with nature. Experience in the field or in a position that ...Conservationist Documentary Director Parasitology Botanist Nature Photographer/Artist Ecologist     Computer...Include any relevant job specific ‘buzz words’ to ensure recruitment agencies and employers put your resume...

...and with a group who all strive for a common big-picture goal. It is worth doing some some serious pre-work...position. Am I choosing the right mentor? Make sure your advisors will be good role models and teach... career path?  If you are considering this, make sure to choose an advisor that supports both paths – ...details and many more suggestions on planning for future success by finding and joining your dream lab, ...

...pitfalls? Here's what you need to know.   Figure 1: Management can seem a mystery at first! Image...potential. I often thought that performing a cell culture experiment myself would take less time than the...independence, while others required more guidance and structure. I quickly learned that my approach needed to ...approaches—one detail-focused and the other big-picture oriented. Their misalignment was slowing progress...

...pandemic. Though we’ve confirmed the expected features and sequences in each plasmid, none of these constructs...will give your research more visibility.    Figure 1: Illustration of SARS-CoV-2 from Philippa Steinberg... plasmids to confirm they contain the expected features and are free of mutation.  Once the samples arrive...scientists to confirm the plasmids contain all expected features and meet our QC standards. If any concerns are...

... conference via social media. The podcast also features an interview with Dr. Stephani Page, the founder...about a week and then unfollow those who do not capture your interest during that week.” You can also find... Media as a Scientist: A Very Quick Guide" from Nature Jobs Blog While Twitter is a popular platform for...pointers on how to use LinkedIn to research potential future employers and to arrange informational interviews...

...distribution and interactions of proteins within these structures. Enzyme-catalyzed proximity labeling (PL) uses...limitations of APEX2 and BioID. In the Ting Lab’s recent Nature Biotechnology paper, they describe TurboID and ..., the enzymes function across a much broader temperature range than BioID, which only functions optimally... the SH3 domain collection here! Teyra et al. Structure 2017. Pubmed PMID: 28890361. ...

...can affect reported titer by either method so to ensure equal animal dosing, we advise that users keep ...into codelivery efficiency at different doses. See Figure 4 in this paper for more information.   Q6 & Q7...Brain potency for each individual is reported in Figure 4B (see left for image and link for paper.)    ...and smaller CRISPR tools is an exciting area for future AAV application to epigenetic editing. This area...

...preceded by GA or followed by TC. As shown in the figure below, a Dam methylase recognition site (underlined... diagnostics, we suggest double checking to make sure your results will not be affected by methylation... has the XbaI site blocked by methylation), the figure below illustrates how sequence visualization software...will be blocked by Dam or Dcm methylation, you can ensure this site remains unblocked by first cloning your...

...are required for NHEJ repair regardless of end structure, and dictate the major events of the pathway: ...polymerases (Pol μ and Pol λ), nucleases (Artemis), and structure-specific end cleaning enzymes (Aprataxin, Tdp2... obligatorily introduce indels. Given the end structure of the Cas9 DSB (blunt or near-blunt ends without...graduate student interested in determining how the structure of broken ends impacts how they are repaired. ...

...engineering technology. Monoculture and chemical spraying, mainstays of modern agriculture, are now synonymous... “GM” by demonstrating the current benefits and future potential of the technology to the public in an...enjoyed exploring the outdoors and experiencing nature first-hand. He studied biology at Virginia Tech...

...for vector propagation. This post, along with a future companion post on mammalian vectors, will catch... you up on the core replication and resistance features of yeast vectors and explain how they differ from...eukaryotes and thus contain complex internal cell structures similar to those of plants and animals. Unlike...Saccharomyces cerevisiae; however, we’ve also noted some features required for the replication of fission yeast,...

...Addgene is ending 2024 with big plans for our future. And while we’ve been excited to share them with...now-common business practices, like accepting e-signatures and designing a website users could easily navigate...grow accessibility alongside our repository.  “I’m sure you hear it all the time, but Addgene is a totally...institutions with standardized agreements and electronic signatures to dramatically speed up MTA approvals. In 2013...

...before me had been assigned a structure paper with two single-panel figures, one of which was a cartoon ...molecular biology class. A Cell paper. With six figures. Averaging ten panels each. (For this story, it...notebook, opened it, found my outline, read over Figure 4, took a deep breath, and, re-oriented, continued...

...and the electroporated cells re-adhered to the culture plate within 4 hr of treatment. In some primary...super-resolution light microscopy, comes from his recent Nature Communications article “SRpHi ratiometric pH biosensors...to each transcription factor. They then induced cultures of these transformants with various concentrations...predicts that this level of control will enable future researchers to better simulate physiological gene...

...developing assays.  Protocol architecture The basic (very basic!) architecture of the indirect approach is...indirectly, with each protein of interest.    Figure 1: The direct approach (left) versus the indirect...best to use the direct method when possible, or ensure that the primary antibodies are different species...

...antibody you’ll want to be sure to choose the appropriate controls to be sure the staining you are seeing...produced Polyclonal antibodies are a heterogeneous mixture of many antibodies that recognize the same protein...change. If your protein might become slightly denatured during your experimental protocol, using a polyclonal...

...possibly giving you a way to connect with them in the future. Some profiles have a blue Follow button. For less...LinkedIn page. This can include news, inclusion, culture and connections. Start your interview research ...and “plus skills” areas. Include a professional picture. It doesn’t have to be a headshot in a suit, but.... Try looking a bit over your shoulder for the picture. It tends to look like a police photo if you look...

...context. While native purification preserves RNA structure and naturally occurring complexes present in the...which for RaPID is an RNA motif of interest. See figure 1 below for details on the key components of RaPID...in breast cancer patients. By digging into the literature and analyzing data from The Cancer Genome Atlas...–protein interaction detection in living cells. Nature Methods, 15, 207-212. PubMed PMID: 29400715. PubMed...

...fluorescent in situ hybridization (FISH) and chromosome capture suffer from low resolution and can’t be used in...using all the colors of the CRISPRainbow. Figure 1: CRISPRainbow was used to track the localization...image represents the composite of fluorescent measurements from red, blue, and green channels overlaid ...with dCas9 supplied by a separate vector. Figure 2: CRISPRainbow generates 6 unique colors and a...

...plasmid, succumb to the toxicity of CcdB.   Figure 1: Image from Bernard, P., et al. showing the pKIL...still express ccdB and therefore would die. This procedure dramatically reduces the number of clones that...contain the desired insert should not grow.   Figure 2: Image from http://teachline.ls.huji.ac.il/72682...all of the players becomes ever more important to ensure that you choose the most appropriate method for...

...CARM1 methyltransferase. This attendant signaling feature afforded by coactivator complexes suggests that...the NURSA website, the new version of which will feature a simplified user interface, expanded numbers of...associated with identifying plasmids in the research literature and acquiring them in a timely manner. Addgene...accordingly, the new version of the NURSA website will feature direct links to listings of available plasmids ...