Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 879 results for: IND

Showing: 761 - 800 of 879 results

  1. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    Published
    Aug. 11, 2020, 1:15 p.m.
    ...inherently unstable due to their secondary structure, palindromic nature, and high GC content. This instability...expression in retinal cells Resources on Addgene.org Find AAVs for your experiment Browse our AAV protocols...

  2. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    Published
    March 28, 2022, 4:30 p.m.
    ...neuroscience and is available to both academic and industry scientists.  The collection of antibodies available...As we build the collection, we hope that you will find useful antibodies for your research. Please consider...

  3. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ...your selection method kills the transgene free individual. For example, with CRISPR-Cas9 gene editing,...experiments can only be collected from heterozygous individuals. Only 1/4 of the descendants of the heterozygous...

  5. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    Published
    Aug. 22, 2023, 1 p.m.
    ...piece of equipment that is custom-built, hard to find, or inaccessible to you for another reason. Visiting...visiting position. What now? First, you’ll need to find someone who is willing to host you! If there is ...

  6. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...recombinant proteins by the pharmaceutical and biotech industries as a protein expression workhorse. This yeast...into methanol and peroxisomal autophagy can be induced by removing methanol from the media. This feature...

  7. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    Published
    Feb. 9, 2021, 2:15 p.m.
    ...get to annotate things as they come up in their minds. The final screen can also be saved and exported...PhDs: Swimming upstream a career from academia to industry”. To date, aside from doing science, he has assisted...

  8. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    Published
    Nov. 7, 2019, 2:18 p.m.
    ...journals and publishers. As journal editors, we often find ourselves having to ask authors for additional information...Press. Additional resources on the Addgene blog Find more ways to make your research more reproducible...

  9. How to Lead a Great Meeting

    Type
    Blog Post
    Published
    Feb. 9, 2016, 3:30 p.m.
    ...free to play around with different strategies and find out what works for your organization. Ask for feedback...Advice from Addgene Executive Director Joanne Kamens FInd Tips on Picking a Research Project Read Other Inside...

  12. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ... the only informal synthetic biology club of its kind, running from start to finish during the academic... Addgene.org Guide to SynBio Assembly Standards Find Plasmids for Creating and Controlling Gene Networks...

  14. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...method it is not possible to follow turnover of individual proteins in situ.  For the visualization of specific...Söderberg, Ola, et al. "Direct observation of individual endogenous protein complexes in situ by proximity...

  15. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ...request from us but also by high school students and industry scientists as a universal resource for information...about Addgene Resources on the Addgene website Find plasmids for your research  Learn about our viral...

  16. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...Another similarity to Cas12a is that Cas14 also indiscriminately cleaves non-complementary ssDNA when engaged... the genome. Cas13, similar to Cas14, also indiscriminately cleaves ssRNA when engaged at its RNA target...

  17. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ...research (e.g., AAV) are useful for labeling the individual neurons they infect. However, they generally ...Expression AAV for Rabies Glycoprotein (G) expression  Find Retrograde AAV ...

  18. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ...decontaminating liquid waste, remember that the percentage indicates the proportion of the decontaminate in the waste...things they work with, and that can understandably induce some nerves. Following safety procedures and practicing...

  19. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ... question falls. Here are some things to keep in mind after you hit “send” or dial that last digit: An...why you haven’t received a response, just keep in mind that our customer support scientists wear many hats...

  21. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ...challenging to compete in today’s tough academic or industry job market for biology PhD’s. I was facing the...plants engineered to detect explosives and rapidly indicate their presence. They are genetically modified ...

  23. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ... Sybr Green is a fluorescent dye that works by binding to all double-stranded DNA, while Taqman uses an...variance in your variant options, you’re likely to find an application that matches your needs. Happy quantifying...

  24. Antibodies 101: Flow Compensation

    Type
    Blog Post
    Published
    May 16, 2024, 1:15 p.m.
    ...wavelengths (See this handy emission spectra chart to find your antibody's spectra). Your cytometer will therefore...diagonal population, this is a sign of spillover and indicates that it is necessary to apply a compensation value...

  25. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...the University of Miami Miller School of Medicine. Find plasmids from the Lee Lab here. Pantelis Tsoulfas...the University of Miami Miller School of Medicine. Find plasmids from the Tsoulfas lab here.     References...

  26. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ... to build a gene drive in a model organism would find our work and consequently use these precautions ...geographic area where escaped organisms won't be able to find mates2. For example, ongoing experiments attempting...

  27. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...-expression by using a plasmid with multiple, individual expression cassettes, having the genes expressed... the ability to spark translation initiation independent of the 5’ cap.  IRES elements are very useful...

  29. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...2015). Total Virus and Bacteria Concentrations in Indoor and Outdoor Air. Environmental Science & Technology...RT and IN incorporated into HIV-1 particles independently of the Gag/Pol precursor protein. The EMBO Journal...

  30. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ... insert-specific sequence. This is a “yes or no” kind of test, with a positive clone amplifying a product...insert-specific and back-bone specific primers. Find Empty Backbones at Addgene Each approach has advantages...

  31. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ...collaborators have completed their work thoroughly. It’s kind of a chicken and egg situation- do we work so hard...rose-colored-glasses-esque picture of how everyone is kind and collaborative and all we do is have fun all ...

  32. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...information or clarification on these vectors. Read on to find the answers to our most frequently asked lentiviral... designed specifically for transient expression (find some useful backbones here) or from viral transduction...

  34. New Coomassie Protocol Video

    Type
    Blog Post
    Published
    Oct. 31, 2023, 1:15 p.m.
    ... proteins.    If this video is helpful, you can find our complete collection of protocol videos on the...

  35. New Western Blot Protocol Video!

    Type
    Blog Post
    Published
    March 28, 2023, 1:15 p.m.
    ...out all of our protocols on our Protocol page, and find our protocol videos on our YouTube page.     ...

  37. Introducing...Addgene Quick Tips!

    Type
    Blog Post
    Published
    Sept. 19, 2024, 3 p.m.
    ...Bench Layout,” is below.      And you’ll be able to find all published videos in our Quick Tips playlist:...

  38. Help Us Name Blugene’s New Virus Friend

    Type
    Blog Post
    Published
    Jan. 7, 2020, 1:55 p.m.
    ...Hmmm, they need a name. And we need your help in finding a good name for Blugene’s virus friend.  During...
Showing: 761 - 800 of 879 results