We narrowed to 812 results for: cha;

...will be blocked by methylation and how that may change your expected digest results. If Dam methylation...

...pUC19 Let’s begin at the beginning. The well-characterized bacterial lac operon contains a gene called ...

...different biological systems. Although Xie et. al characterized the system in plants, PTGs will likely work ...

...CRISPR technology has changed how scientists edit and control genes, but according to the Broad Institute's...

... can also see and offer congratulations on job changes and promotions.  Follow academic scientists on ...

...26814968. McCarty, D. M., Paul E. Monahan, and Richard J. Samulski. "Self-complementary recombinant adeno-associated...

...small size of the polyhistidine tag minimizes the chance of the tag disrupting normal protein function, ...

...student groups and being aware of cognitive load changed the way I thought about engaging with learners....

...protein of interest is often one of the most challenging components of any immunoimaging assay. Secondary...

...In 2016, I was put in charge of the biggest project I’d seen in my already long tenure at Addgene. I ...

...the previous reactions. This will increase your chances to have a successful and ultimately faster cloning...

...29608282. PubMed Central PMCID: PMC5963681.  Meyer, Richard. "Replication and conjugative mobilization of broad...

... On the other hand, slow growth can also cause changes in plasmid copy number. During the shift from exponential...

... Michael G. Lemieux...

...from Promega, including many HaloTag plasmids Exchangeable HaloTag Ligands HaloTag Reversible binding of...

...promotes gender equality in careers through structural change in research organizations. “Fix the Knowledge” ...

...has been successfully applied to functionally characterize combinatorial gene knockouts generated via multiplexed...

...would have different labels - i.e. they would stochastically arise from different cells throughout the treatment...

...already-existing sets. Note that this tool relies on a stochastic search algorithm rather than pre-designed sets...

...familiarize yourself with your organization’s reporting mechanisms before you start an experiment, just in case ...

...application (different experiments/conditions can change binding ability – i.e., the antibody can no longer...

...Cas9 [other], Cas12a, RNA-targeting) CRISPR mechanism (KO, CRISPRa, CRISPRi, editing)   Figure...

... Michael G. Lemieux...

... number or expression level effects.  Another challenge that may arise when crossing mice with multiple...

...information briefs for policymakers. The rapidly changing landscape of genetics and our consistent stream...

...Tsiang H, Ceccaldi PE. Spread and pathogenic characteristics of a G-deficient rabies virus recombinant: ...

...Institute website at alleninstitute.org. A classic challenge in cell biology is making sure that what we observe...

...repression, and editing with a single Cas9-VPR in Saccharomyces cerevisiae. To control Cas9 activity and influence...

...No conference would be complete without CRISPR Michael Terns from of the University of Georgia studies...

... case.   Funding Makerspaces One of the main challenges when starting a new project at a makerspace is...

... genetic redundancy (meaning, there’s more of a chance of seeing a phenotype since additional genes that...

...CRISPR, siRNA, etc. Firstly, they are reversible mechanisms of control. Protein degradation can be induced...

...in house using a simple PNK reaction or can be purchased 5’-modified from a company. However, unlike an...

...The backbone modifications listed can refer to changes made to the original backbone by the depositing...

...model.  If your expression system is not well characterized in terms of CRISPR use, you will want to invest...

...this type of experiment can shed light on the mechanism by which cells gain resistance to drugs and can...

...enjoy seeing how our quality control process has changed with the introduction of next generation sequencing...

... like other viral vectors, though significant challenges remain (Wold & Toth, 2013).  While the pre-existing...

...domains of a protein that undergoes conformational change. Typically, two fluorescent proteins with overlapping...

...from flow cytometry experiments can be a little challenging to understand if you’ve never done any flow. ...