We narrowed to 610 results for: ACE;
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TypeBlog PostPublishedMarch 6, 2018, 1:49 p.m....and the ATP dependent 9°NTM DNA Ligase are not replacements for T4 DNA Ligase in applications such as routine...PMCID: PMC306403. 11. Wu, Dan Y., and R. Bruce Wallace. "Specificity of the nick-closing activity of bacteriophage...
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Antibodies 101: Designing Your First Flow Panel
TypeBlog PostPublishedMay 28, 2024, 1:45 p.m.... presence or absence of certain markers on the surface or the inside of your cells. While proteins themselves...plasma membrane and can be detected through a cell surface label. The viral vector also encodes for eGFP, ... -
Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands
TypeBlog PostPublishedMarch 21, 2024, 1:15 p.m..... Other dyes may prove challenging to stain intracellular compartments in in vivo model systems, so extra...green, red, and far-red dyes is important for intracellular fluorescence live imaging (Hayashi-Takanaka... -
Typing CRISPR Systems
TypeBlog PostPublishedMarch 18, 2025, 1:15 p.m....function. After analysis, CRISPR classifications were placed into a fairly simple hierarchy: class to type to.... However, knowing the basic diversity and the surface-level function of each type could lead you to new... -
Deep Dive: qPCR
TypeBlog PostPublishedSept. 29, 2022, 1:15 p.m....multiplex your reference genes to save on plate space. For many cell lines and tissue types, appropriate...your reference gene set can save significant plate space and may be worth the initial effort of setting it... -
Technical Design of a Western Blot
TypeBlog PostPublishedAug. 22, 2024, 1:15 p.m....stability Limited protein size range Tris-acetate 40–500 kDa Tris-tricine 150 V, 1–3 hours ... simply wet your membrane with 20% methanol and place it on a light box after drying. Nitrocellulose, ... -
Addgene’s First AAV Data Hub Challenge
TypeBlog PostPublishedSept. 20, 2022, 1:15 p.m....Addgene’s first AAV Data Hub Challenge, now taking place Dec. 6, 2022! Share your AAV data, connect with ... -
Introducing the Addgene Antibodies 101 eBook!
TypeBlog PostPublishedOct. 29, 2024, 1:15 p.m....application question, the Antibody 101 eBook is a great place to start looking for answers. We hope you find... -
Meet Aavery, Blugene's New Virus Friend!
TypeBlog PostPublishedFeb. 13, 2020, 2:34 p.m....vector terms. And, the name Kendall has a special place in our hearts: our first two offices were located... -
Addgene is Stopping Use of X (formerly Twitter)
TypeBlog PostPublishedJan. 11, 2024, 2:15 p.m....addgene.bsky.social; or @addgene on Instagram, TikTok, Facebook, and LinkedIn. We are also exploring other social... -
The 12 Days of CRISPR: 2021
TypeBlog PostPublishedDec. 28, 2021, 2:15 p.m....Addgene gave to me: the PEDAR strategy to delete and replace long genomic sequences. On the ninth day of CRISPR... -
Overwhelmed? Take a Break With Our 5 Favorite Science Comics
TypeBlog PostPublishedDec. 5, 2013, 3:27 p.m.... science and in the lab? Addgene is after all a place that encourages people to take some time out every... -
Twelve Amazing Guest Blog Contributors!
TypeBlog PostPublishedNov. 26, 2024, 2:15 p.m....Cytometry, Designing Your First Flow Panel, and Beyond Surface Labeling Ashlyn Lemmen, who shared her flow cytometry... -
Addgene’s New Way to Help You Share Science
TypeBlog PostPublishedNov. 14, 2013, 4:10 p.m.... at [email protected] or tell us on Twitter and Facebook. We’ll track down the story, get the inside scoop... -
Bringing Renewable Energy to Addgene
TypeBlog PostPublishedAug. 27, 2019, 1:08 p.m..... Addgenies swapping out printer cartridges can place the spent cartridge in the large cardboard box in... -
Addgene Protocols Go Viral: AAV Purification and Titration
TypeBlog PostPublishedJune 13, 2019, 12:19 p.m....! AAV purification by iodixanol gradient ultracentrifugation This video goes over AAV vector purification... -
Deck the Lab 2022!
TypeBlog PostPublishedJan. 17, 2023, 2:15 p.m....It's just unfortunate that we had no choice but to place it next to our lab trash (which coincidentally matches... -
Tips for Working in Industry & Success in Management: Interview with Crystal Shih
TypeBlog PostPublishedOct. 5, 2017, 1:13 p.m....Investigator III at Novartis, a multinational pharmaceutical company based in Switzerland. After completing... -
CRISPR Cheat Sheet
TypeBlog PostPublishedJan. 27, 2021, 2 p.m....! CRISPR Cheat Sheet Clustered Regularly Interspaced Short Palindromic Repeats: Sequences of DNA found... -
Countdown to Halloween @Addgene
TypeBlog PostPublishedOct. 29, 2014, 5:26 p.m....this year’s Addgene costume contest, like us on Facebook and checkout our FB page on Friday for more photo...