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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 836 results for: Abo;

Showing: 381 - 400 of 836 results

  1. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    Published
    Sept. 14, 2017, 12:40 p.m.
    ..., see the table below and read on to learn more about GeoCas9.   SpCas9 GeoCas9 Size 1368 AA 1087...vivo. What aspect of GeoCas9 are you most excited about? Thermostability? Longevity in human plasma? Something... Additional Resources on the Addgene Blog Read about CRISPR Methods for Bacterial Genome Engineering   ...

  2. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

    Type
    Blog Post
    Published
    Aug. 22, 2019, 12:43 p.m.
    ... 101 blog posts Learn more about Lambda Red recombineering Learn more about counter-selection Resources...levansucrase that converts sucrose into a toxic metabolite in gram-negative bacteria. Plating on sucrose...

  3. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

    Type
    Blog Post
    Published
    Dec. 13, 2017, 3:20 p.m.
    ...ReFigure Analyzing replications as in this ReFigure about whether Natronobacterium gregoryi Argonaute has ...functionality Collecting preclinical and clinical data about a disease or therapeutic Side-by-side comparison...share your science at We Share Science Learn more about Data Sharing Resources at Addgene.org Deposit ...

  4. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    Published
    Dec. 3, 2020, 2:15 p.m.
    ...engineered Learn about the parts of the AAV transfer plasmid  Resources on Addgene.org Learn about AAV Find ...plasmid! Check out this blog post to learn more about the AAV transfer plasmid! References: Naso MF...

  5. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    Published
    March 31, 2015, 4:08 p.m.
    ...that their teams are working well together and collaborating effectively with other teams. While they sometimes... they are as engaged as possible. To learn more about being an effective manager, read the other posts...our podcast interview with Connie Cepko to learn about her management style. Leaders look to the future...

  6. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ...molecular biology, yet scientists rarely stop to think about how much easier it makes our lives. Plasmid transformation... sterile filter unless otherwise specified. The above table lists some antibiotics commonly found in the...or light – do not add them to media hotter than about 55oC and store plates/stocks wrapped in foil if ...

  7. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    Published
    Jan. 24, 2019, 2:24 p.m.
    ...Additional resources on the Addgene blog Read about Brainbow Learn about using AAVs for neuronal tracing Visit...page Additional resources on Addgene.org Learn about Addgene's viral service See our fluorescent protein...

  8. The Golden Stopwatch Award: Recognizing Facilitators of Research

    Type
    Blog Post
    Published
    Sept. 5, 2019, 1:06 p.m.
    ...materials as quickly as possible. (To learn more about why MTAs matter, check out another blog post: Why...provide specific examples if possible Tell them about the Golden Stopwatch! Any organization that deposits...California Institute of Technology Cold Spring Harbor Laboratory  Dana-Farber Cancer Institute  Fred Hutchinson...

  9. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    Published
    Feb. 14, 2018, 2:36 p.m.
    ...most popular tweet from the conference is shown above. The blog post I mentioned, John Doench’s How to...forward. To do that, we need to learn much more about cellular DNA repair pathways. An emphasis on cellular...at the Keystone meeting had great things to say about CRISPOR, a tool to help you design, pick, and close...

  10. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    Published
    Dec. 21, 2017, 2:06 p.m.
    ...with users of the software. We welcome suggestions about common features from anyone who uses SnapGene or...Product Management at SnapGene. She is passionate about building software that people love to use, and likes...  Additional Resources on the Addgene Blog Read about our SnapGene powered plasmid maps Learn how we use...

  11. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ...during development. Read this post to learn more about recombinase-based state machines and how they function...Albert Cheng and Mark Wanner from the Jackson Laboratory for Genomic Medicine described Casilio, a system...your target gene. Read this post to find out more about how the Casilio system works and how you can customize...

  12. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    Published
    June 9, 2016, 2:30 p.m.
    ...impaired NgAgo activity, with three mismatches abolishing activity. One-guide faithful NgAgo guide loading... how it’s working in the comments section! Read About NgAgo User's First Impressions Here References ... Additional Resources on the Addgene Blog Read About Cas9 Homolog Cpf1 Check Out High Fidelty Cas9 Mutants...

  13. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ...eyes open for any new technologies that will bring about the next revolution in biological research. We’re...Plasmids 101: Control Plasmids Need help thinking about the types of controls you’ll need for your next ...Addgene Browse Our CRISPR Resources Learn More about Viral Vectors Browse the Repository  ...

  14. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2015, 2:30 p.m.
    ... in part to the fact that very little was known about the role these factors played within an organism...genetics. This all changed thanks, in part, to a collaboration initiated at a Hawaiian deli in 1972. Using ...pictures and pro tips.  Happy reading! Learn more about the history of plasmids and cloning: CSHL Meeting...

  15. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    Published
    July 7, 2015, 3:36 p.m.
    ...just one injection step! The entire protocol takes about 2-3 weeks but requires less than eight hours worth... this species, namely, the robust HDR mentioned above, an accessible germline, and multiple selectable...modular system that requires very little hands-on labor and could facilitate the tagging of every protein...

  16. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    Published
    Aug. 21, 2014, 3:15 p.m.
    ...for Dummies”. The fastest way to learn something about marketing would be to start reading a broad simple...introduction to the financial world. It teaches you about selling products which is an important part of the...Seán on twitter @Macfhearraigh.    Read more posts about Scientific Career Development ...

  17. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    ...determining the T-cell epitopes that will likely bring about unwanted immunogenicity. The 3-D models can be applied...limitations. Using methods together can inform decisions about in vivo application and clinical testing. This would...to drugs: adverse reaction terminology. WHO Collaborating Centre for International Drug Monitoring, Uppsala...

  18. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...pombe have dominated the research scene. But what about the other yeasts – in the biomedical world, are ...pharmaceutical companies (along with some research laboratories) have picked up the system for some of the uses...eukaryotic cells and peroxisomes are where methanol metabolism occurs in methylotrophic organisms. K. phaffii...

  19. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    Published
    April 9, 2020, 1:15 p.m.
    ...following up on these leads as we speak, with collaborators in Paris and New York. We want YOU to join the...impact on virus replication. While we and our collaborators are starting these experiments, there's so much...are particularly interested in facilitating collaborative science, and making it accessible to all people...

  20. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ...Download Addgene’s Science Career guide to learn more about career paths and things you can do to prepare. ...can adapt to your kids' needs. Plan ahead. Think about and set up activities that kids can do more independently...leading up to your defense less stressful. Think about reproducibility best practices Support your future...
Showing: 381 - 400 of 836 results