We narrowed to 530 results for: Erf

1. Viral Vectors 101: Gamma-Retroviral Packaging Systems

   Type

   Blog Post

   Published

   Aug. 6, 2024, 1:15 p.m.

   ... in gene therapy research to reduce promoter interference and/or drive high expression of a transgene ...

2. OCaMP: A New Calcium Indicator for Neural Imaging

   Type

   Blog Post

   Published

   Aug. 19, 2025, 1:15 p.m.

   ...CC-BY-NC-ND 4.0 International license. Better performance = better data In side-by-side tests with popular...

3. Viral Vectors 101: Viruses as Biological Tools

   Type

   Blog Post

   Published

   March 21, 2023, 1:15 p.m.

   ...viruses such a pain is exactly why viruses are such wonderful scientific and medical tools. As scientists, we...

4. Sharing is Caring: But How to Distribute Open Hardware?

   Type

   Blog Post

   Published

   Feb. 28, 2023, 2:15 p.m.

   ...its early years, the light microscope has been a powerful tool in almost all disciplines. In biology, innovative...

5. Plasmids 101: Methylation and Restriction Enzymes

   Type

   Blog Post

   Published

   June 30, 2016, 2:30 p.m.

   ...How can I tell if my enzyme will cut? Whether performing a digest for cloning purposes or for diagnostics...

6. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

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   Blog Post

   Published

   Feb. 10, 2016, 3:30 p.m.

   ... Molecular Biology Reference Pages Choose Your Perfect Plasmid Backbone Find Plasmids for Your Research...

7. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

   Type

   Blog Post

   Published

   July 28, 2015, 2:30 p.m.

   ...quite small—20 amino acids—so we don’t think it interferes with the function of the protein. Myers: We’ve...

8. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

   Type

   Blog Post

   Published

   Feb. 24, 2015, 7:50 p.m.

   ...treatment with a BRAF inhibitor. (The test was the perfect complement for one conducted earlier by the Zhang...

9. dTAG - You're it!

   Type

   Blog Post

   Published

   June 21, 2018, 2:06 p.m.

   ...dTAG-13 due its immediate action, potent ligand performance, great depth of degradation, absolute selectivity...

10. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type

    Blog Post

    Published

    Sept. 8, 2020, 2 p.m.

    ...will use Addgene’s RESOLUTE plasmid collection to perform a functional screen in mammalian cells to identify...

11. RNA Extraction Without A Kit

    Type

    Blog Post

    Published

    March 24, 2020, 1:15 p.m.

    ... to avoid contamination, take extra care when performing RNA extraction. RNA is inherently not as stable...

12. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type

    Blog Post

    Published

    March 3, 2020, 2:15 p.m.

    ...CreLite-mediated recombination was found in both deep and superficial tissues of zebrafish studied, such as the brain...

13. CRISPRainbow and Genome Visualization

    Type

    Blog Post

    Published

    Feb. 28, 2017, 3:30 p.m.

    ...BioRender.com. With this rainbow of colors, Ma et al. performed sophisticated tracking of multiple chromosomal...

14. Troubleshooting Your Plasmid Cloning Experiment

    Type

    Blog Post

    Published

    Sept. 24, 2019, 12:55 p.m.

    ...primer to 40-60 bp. It is always a good idea to perform primer optimization, especially if you are having...

15. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type

    Blog Post

    Published

    March 19, 2019, 1:31 p.m.

    ...state of the photoswitchable proteins offers a powerful approach to control protein-protein interactions...

16. Celebrating 15 Years of Scientific Sharing

    Type

    Blog Post

    Published

    Jan. 14, 2019, 2:08 p.m.

    ... At Addgene we will never ever stop planning, perfecting, and learning, but there are a lot of things ...

17. Giving gRNAs a Facelift - Synthetic and Beyond

    Type

    Blog Post

    Published

    Oct. 20, 2022, 1:15 p.m.

    ...modification(s) Congratulations! You’ve picked your perfect guide; now check out our blog posts on choosing...

18. Antibodies 101: Affinity Reagents

    Type

    Blog Post

    Published

    April 14, 2022, 1:15 p.m.

    ...exciting time to be a scientist looking for that perfect fit!  References and Resources Additional resources...

19. Antibodies 101: Selecting the Right Antibody

    Type

    Blog Post

    Published

    March 15, 2022, 1:15 p.m.

    ...Antibody-based assays are complex and antibodies may perform differently in different samples and experimental...

20. Antibodies 101: Polyclonal Antibodies

    Type

    Blog Post

    Published

    July 14, 2021, 1:15 p.m.

    ...immunogen. A different type of purification can be performed to isolate antibodies only recognizing your protein...