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We narrowed to 450 results for: HA tag

Showing: 381 - 400 of 450 results

  1. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    Published
    March 30, 2017, 2:30 p.m.
    ...emerging validation technology. Digital PCR has several advantages over mismatch nuclease assays that will...As mentioned, these assays have several major advantages over the commonly employed mismatch nuclease ...bi-allelic mutations: Perhaps the most noteworthy advantage of digital PCR assays is that they are easily .... 6. Fu, Y. et al. High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells...

  2. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    Published
    Feb. 18, 2014, 4:22 p.m.
    ...senior mentors available to go around. My advice has been to find 5 interested peers and form your own...step-by-step, how-to guide to get one started. Advantages of the group mentoring format Adding peer advice...Because the participants are at a similar development stage, they have a lot to learn from each other. Some ...the group. The group can be one gender and career stage (e.g. all women, all men, all postdocs, all grad...support each other as you transition to the next stage in your careers! Stay in touch…this group will be...

  3. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    Published
    July 21, 2020, 1:15 p.m.
    ...quantified at 595 nm. This method is time consuming and has low sensitivity so it’s not used much. However, the...concentration. It’s important to consider the advantages and disadvantages of each method and when one method might...

  4. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    Published
    Nov. 5, 2019, 1:28 p.m.
    ...the most widely used CRISPR nuclease, recent work has shown that alternative enzymes may better suit specific...Joung and Jennifer Doudna Labs used targeted mutagenesis to generate hyper accurate Cas9 (HypaCas9). This... sites. Using a combined random and directed mutagenesis approach, the Anna Cereseto Lab created pX-evoCas9...experimental approaches, knowledge of both the advantages and limitations of each Cas enzyme can help determine...

  5. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...since its development, CRISPR/Cas9 genome editing has been used to study the effect of gene knockout in...-target effects and/or toxicity, it would be advantageous to instead use a preexisting, conserved cellular...leading nucleotide could be processed, a distinct advantage over other methods that favor certain nucleotides...potential of PTGs. Applications of PTGs The major advantage of the PTG system is its universality; robust ...

  6. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    Published
    July 10, 2014, 7:17 p.m.
    ...Collin’s Lab in 2009. From 2009 to today, Sample6 has changed focus and names, swapped leadership, and ...of technologies, effectively communicating the advantages embodied, and driving the enterprise value of...plan competition and advance investors to the next stage of diligence." In business competitions everything... be OK with the constant fear of failure at all stages in the startup". To gain insight into "the random-walk...

  7. "What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring

    Type
    Blog Post
    Published
    Dec. 17, 2013, 2:47 p.m.
    ...?” Some of the Tweeted comments: “A good mentor has your goals/needs/situation in mind, rather than (...being coddling, to listen without being taken advantage of, and to respect my students as developing colleagues...

  8. What's New in CRISPR - September 2019

    Type
    Blog Post
    Published
    Sept. 10, 2019, 12:56 p.m.
    ...PAM motif, averaging 19 targets per gene. The lab has previously used the library with SpdCas9 to perform...lab discovered a new Cas protein, CasX, from metagenomic analysis of microbial DNA from groundwater. Now...

  9. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    Published
    Aug. 23, 2022, 1:15 p.m.
    ...with chemicals in it,” leaving me to wonder how he has survived this long without downing any dihydrogen...graduate with my BSc in Anatomy, the University of Otago had established the Centre for Science communication...Department of Science Communication at the University of Otago. Her research focuses on misinformation about sexual...

  10. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...AAV for your experiment. The Addgene viral service has now expanded to offer many of our control plasmids...tools for light mediated neuronal control. The advantages of optogenetics lie in the spatiotemporal control...recombination of lox sites. This system takes advantage of the extensive supply of transgenic reporter...

  11. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    Published
    Oct. 15, 2024, 1:15 p.m.
    ...titers found in our in-stock preps. If a request has technical considerations that may affect expected...specialist will reach out to you during the request stage, before you order.  Timeline Once your order is ...

  12. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    Published
    April 1, 2014, 5:33 p.m.
    ...to a variety of careers outside of academia. She has had previous graduate students and postdocs go on...interviewed Addgene depositors who are at various stages in their careers to get advice on choosing a lab...

  13. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    Published
    June 4, 2020, 1:22 p.m.
    ...amplification are endA negative, meaning the gene has been removed/mutated to neutralize the nuclease activity...elution of plasmid DNA through their kit. The advantage of using a Tris buffer is that it allows better...

  14. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ...logical pattern across the poster. This style has the advantage of being different and potentially more catchy...to your audience. With a poster, you have the advantage of being able to verbally communicate your work...and acknowledgements sections are inserted. The advantage of this style is that it will display your data...

  15. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ...nucleases to generate an engineered mouse model has advantages over the injection of a targeting vector alone... to create engineered mouse models at embryonic stages, and the pros and cons of different genome editing...mice are engineered during the embryonic stem cell stage using one of several techniques. The longest standing...

  16. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    Published
    May 23, 2019, 12:26 p.m.
    ...platform. During the conference If the conference has a social media policy, this should be on their website...conference hashtag to set one up. It’s also helpful to write your Twitter handle on your nametag for the ...Check out the conference website for any relevant hashtags and handles to help you find other scientists ... share their research on social media. Use the hashtag if you are live-tweeting any sessions that the ...

  17. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...’s a good thing, though! Viral delivery has several advantages over other mechanisms. For one, many viral...Cellular phenomena such as exocytosis, autophagy, voltage, and tension all have specific sensors designed...

  18. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    Published
    Nov. 29, 2016, 3:30 p.m.
    ... is easy, after all, who working in a laboratory has never faced an odd situation with experiments? Our...major challenge when we started was creating a LEGO stage that resembles a real laboratory. It only took one...

  19. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ...In these instances, the cloning vector typically has two separate resistance cassettes and your gene of...Additionally, the presence of a plasmid is disadvantageous from the bacterium's perspective – a plasmid-containing...

  20. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ..., another method to create point mutations, also has a strict editing window about 15 nt upstream of the...NGAG and NGCG and SaCas9 with an NNGRRN PAM by mutagenizing the PAM-interacting domain. However, these alternative...mutants with PAM flexibility. In this system, the mutagenesis plasmid (MP6) permits quick, large-scale mutation...recognizing multiple PAMs should have a fitness advantage over proteins with single PAM recognition. ...cell population measured with NGS) are shown as percentages, with fold changes compared to SpCas9 in parentheses...
Showing: 381 - 400 of 450 results