Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 770 results for: INA

Showing: 381 - 400 of 770 results

  1. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ...DNA preps with multiple samples without cross contamination. We currently use the CosMCPrep system for this...new NEBNext Ultra II FS DNA Library Prep Kit for Illumina sequencing and took a class at NEB’s new teaching...prior NEBNext Ultra II DNA Library Prep Kit for Illumina, one was only able to extract libraries from previously...

  3. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    Published
    April 19, 2017, 3:25 p.m.
    ...assemble hundreds of different plasmids from a single Illumina MiSeq® run. As shown in Fig. 2, below, differences...heterogeneous and/or mixed clones present in the original sample. At seqWell, we have worked with Addgene...prepared as plexWell™ libraries, sequenced on an Illumina® sequencer, and then analyzed with our plasmid...

  4. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    Published
    Dec. 6, 2016, 3:30 p.m.
    ...Cas9 activators available in lentiviral format. Finally, while we can’t currently produce custom gRNA-containing...representation in the viral prep, infect target cells, and, finally, conduct the screen. To help alleviate some of...any questions or comments to [email protected]. Finally we’d like to thank all those who are allowing us...

  5. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    Published
    Aug. 4, 2020, 1:15 p.m.
    ...these materials may not be available from their original sources. Figure 1: Biological repositories...availability of this strain from The Jackson Laboratory. Originally developed and characterized by Stanley Perlman...materials to be deposited into repositories, and financial support to maintain repositories’ capacity for...

  6. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    Published
    June 1, 2021, 1:15 p.m.
    ... credit for small contributions. We strive to eliminate these two barriers, creating a community where...other scientists and to help in Crowdfight’s coordination work.  We also encourage you to file a request...Biomédica de Salamanca (IBSAL) and one of the coordinators at Crowdfight. She is currently based in Spain...

  7. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...Generated against full length GFP and converted to recombinant form by the NeuroMab Facility Isotype IgG2a ...a polyclonal, with the reproducibility of a (recombinant) monoclonal. Another reason is that some antibodies...  et. al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex ...

  8. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...to break membranes of different organisms. And finally, we demonstrated that R bodies can be “tuned” with...F. R. Organization and expression of genetic determinants for synthesis and assembly of type 51 R bodies...trait: Absence of toxicity of R-body producing recombinant E. coli on paramecia. European Journal of Protistology...

  9. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    Published
    Dec. 18, 2014, 4:06 p.m.
    ...how I got my current job as a Communications Coordinator. Wondering how you can develop more transferable...Theresa Liao (@theresaliao) is the Communications Coordinator for the Department of Physics & Astronomy, University...University of British Columbia. Originally on track for a research career, she transitioned into a communications...

  10. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    Published
    June 24, 2014, 3:59 p.m.
    ...Japanese ostracod(Cypridina noctiluca)Sea firefly(Cypridina hilgendorfii) 465 62 Cypridina (Vargulin) luciferin...

  11. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...considered an entirely new cell line from that of the original parental cells. Positive selection in mammalian...  HeLa, NIH3T3, CHO, COS-1, 293HEK Inhibits termination step of translation 2-10 ug/mL G418/Geneticin... Inhibits protein synthesis; premature chain termination 1-10 ug/mL Zeocin Sh bla HeLa, NIH3T3, CHO,...

  13. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    Published
    July 28, 2017, 1:30 p.m.
    ... by geneticists to study gene function. In this final installment, I’ll provide a brief introduction as...transgenes in different loci allows for genetic recombination so that the transgenes can be inherited on the...Engineering Posts Use FLEx Vectors for Site-Specific Recombination Resources at Addgene.org Browse Drosophila...

  14. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    Published
    June 1, 2017, 2:30 p.m.
    ...since it is impossible to check all possible combinations of biology and equipment. Because the performance...(Goedhart et al, 2011) allows for a precise determination of brightness in cells, under realistic experimental...fluorescent protein in the host cell, which is a key determinant of the practical brightness. Therefore, practical...

  15. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...s ability to process its own pre-crRNA arrays eliminates the need to include multiple promoters to drive...processing, i.e. tRNAs or Csy4 cleavage sites. This eliminates bulk from crRNA expression plasmids resulting...crRNA array (bolded red arrow).  This undoes the original reversing of the direct repeats, leading to expression...

  16. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    Published
    Nov. 24, 2020, 2:15 p.m.
    ...log off for the day. 1:45 PM--Write final report. Work on a final report for a user research project I...help implement solutions for the stakeholders. Finally, software product managers test the solutions with...

  17. Hot Plasmids - May 2022

    Type
    Blog Post
    Published
    May 26, 2022, 1:15 p.m.
    ...method (M-CREATE) by: Brian O'Neill  The Viviana Gradinaru Lab has previously developed the multiplexed version...mutations. These plasmids contain catalytically inactive dCas9 and microbial SSAPs (dCas9-SSAP) which insert...specific transcription response element (TRE) or kinase translocation reporters (KTRs). Find visual barcode...

  18. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...Did you catch our April AAV webinar with Tim Miles, PhD, Director of the CLOVER Center at CalTech? If...are sold in packages of 10…)  If you missed the webinar, you can watch the presentation and Q&A session...recent review.      And that's a wrap on our AAV webinar! Special thanks to our panel of experts, Tim Miles...

  19. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    ...support the community in developing better, more sustainable, easily sharable products. Open-Source Microscopy...makerspace networks and training users via workshops, seminars, or hackathons greatly increases accessibility...brings stronger networking across laboratory and disciplinary boundaries and can allow for innovative organizations...

  20. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    Published
    June 2, 2016, 2:30 p.m.
    ... approach, and conclusions (see above). Keep original files in a separate folder: You will often use...for a single use, save the script as originalName_descriptionOfEdit in the same directory where it was ...that was temporarily used. Just be sure that the original code remains in tact! Take notes Comment in ...
Showing: 381 - 400 of 770 results