We narrowed to 783 results for: Ina

...unsuccessful. Nothing more joyful that getting to the final step and the transformed bacteria refuse to grow...(Jackson ImmunoResearch, 016-050-084; 0.2 μg/ml final). Both rabbit (EMD, AB3596) and mouse (NEB, E8032S...matrices, not so much. Maybe a calcium-independent proteinase site could be used for plasmid version 2.0? Instruments...

...mobilized to figure out how to deliver when labs finally opened.  I was awed by the teamwork and initiative...One function that rose to the challenge was our Finance Team. With Addgene closed for almost two weeks ...for unknown amounts of time, we were facing a financial crisis. As a nonprofit organization, Addgene charges...

...Di Ventura (@barbara_synbio) May 13, 2021 The original AraC transcriptional regulator Their system is...does BLADE have over AraC? When compared to the original AraC system, BLADE is simply more precise. “We...light switch and shuts off just as quickly. The original AraC system, which uses arabinose, requires many...

...Washington University in St. Louis provides a combinatorial indexing strategy that simultaneously tracks...cell type that can differentiate into hepatic or intestinal cell types. MEFs were reprogrammed into iEPs ...2019). Key takeaways of CellTag CellTag is a combinatory cell-indexing tool that combines scRNA-seq and...

...outside of academia, a job as a ‘Cancer Marketing Coordinator’ came up at Abcam. Unlike other job descriptions...scientists think that they are unable to tick these final two boxes, but I knew there would be a way around...marketing gives you a great introduction to the financial world. It teaches you about selling products which...

...This is the fifth and final post in the Addgene Blog Mentoring for Scientists Series. The entire series...SMART.” SMART goals are Specific, Measurable, Attainable, Relevant, and Time-bound. It is a good idea ... open communication Feel valued by giving back Final tips for success Make mentoring meetings a priority...

...community-editable resource for storage and dissemination of information about FPs. FPbase.org FPbase...data to existing proteins when missing, or edit inaccuracies as they find them (anyone can register with ...community-editable database is free from the risk of inaccurate data submissions, so FPbase includes a versioning...

...DNA preps with multiple samples without cross contamination. We currently use the CosMCPrep system for this...new NEBNext Ultra II FS DNA Library Prep Kit for Illumina sequencing and took a class at NEB’s new teaching...prior NEBNext Ultra II DNA Library Prep Kit for Illumina, one was only able to extract libraries from previously...

... a means of altering cell fates and generating dominant phenotypes., Development 118, 401–15 (1993). PubMed... 7. S.-L. Lai, T. Lee, Genetic mosaic with dual binary transcriptional systems in Drosophila., Nat. Neurosci..., L. Liang, L. Luo, The Q system: a repressible binary system for transgene expression, lineage tracing...

...assemble hundreds of different plasmids from a single Illumina MiSeq® run. As shown in Fig. 2, below, differences...heterogeneous and/or mixed clones present in the original sample. At seqWell, we have worked with Addgene...prepared as plexWell™ libraries, sequenced on an Illumina® sequencer, and then analyzed with our plasmid...

...Generated against full length GFP and converted to recombinant form by the NeuroMab Facility Isotype IgG2a ...a polyclonal, with the reproducibility of a (recombinant) monoclonal. Another reason is that some antibodies...  et. al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex ...

...these materials may not be available from their original sources. Figure 1: Biological repositories...availability of this strain from The Jackson Laboratory. Originally developed and characterized by Stanley Perlman...materials to be deposited into repositories, and financial support to maintain repositories’ capacity for...

... credit for small contributions. We strive to eliminate these two barriers, creating a community where...other scientists and to help in Crowdfight’s coordination work.  We also encourage you to file a request...Biomédica de Salamanca (IBSAL) and one of the coordinators at Crowdfight. She is currently based in Spain...

... sign up here.        New toolkit for N or C terminal tagging of proteins for degradation using AID ...created a new toolkit of plasmids for the N- or C- terminal tagging of proteins with mAID and other tags (...SpCas9-NG with the activation-induced cytidine deaminase  Target-AID-NG, which mediated a C to T conversion...

..., cyan, or magenta) depending on the hairpin combination. A gRNA containing all three hairpins produces...generates blue, green, or red colors for labeling. A combination of MS2, PP7, or boxB sequences in the same sgRNA...increase stability and minimize misfolding and recombination during virus production needed to deliver CRISPR-Sirius...

...Cas9 activators available in lentiviral format. Finally, while we can’t currently produce custom gRNA-containing...representation in the viral prep, infect target cells, and, finally, conduct the screen. To help alleviate some of...any questions or comments to [email protected]. Finally we’d like to thank all those who are allowing us...

...to break membranes of different organisms. And finally, we demonstrated that R bodies can be “tuned” with...F. R. Organization and expression of genetic determinants for synthesis and assembly of type 51 R bodies...trait: Absence of toxicity of R-body producing recombinant E. coli on paramecia. European Journal of Protistology...

...how I got my current job as a Communications Coordinator. Wondering how you can develop more transferable...Theresa Liao (@theresaliao) is the Communications Coordinator for the Department of Physics & Astronomy, University...University of British Columbia. Originally on track for a research career, she transitioned into a communications...

...considered an entirely new cell line from that of the original parental cells. Positive selection in mammalian...  HeLa, NIH3T3, CHO, COS-1, 293HEK Inhibits termination step of translation 2-10 ug/mL G418/Geneticin... Inhibits protein synthesis; premature chain termination 1-10 ug/mL Zeocin Sh bla HeLa, NIH3T3, CHO,...

... by geneticists to study gene function. In this final installment, I’ll provide a brief introduction as...transgenes in different loci allows for genetic recombination so that the transgenes can be inherited on the...Engineering Posts Use FLEx Vectors for Site-Specific Recombination Resources at Addgene.org Browse Drosophila...