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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 662 results for: MAL

Showing: 381 - 400 of 662 results

  2. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ... from the those used to replicate the host's chromosomal DNA, but they still rely on the host machinery...streaked bacteria have higher copy numbers – for optimal results always pick a single colony and do not ...

  3. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    Published
    Sept. 17, 2020, 1:15 p.m.
    ...to request support for our operations until our normal work resumed. Addgene distributes and fulfills ...supporting research could provide Addgene with a small amount of funds, the community as a whole could ...

  4. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    Published
    Aug. 18, 2023, 4:15 p.m.
    ...decide if they’re alive or not. Yet these simple, small particles have quite the outsized impact — and not...of viral vectors (which many researchers will informally refer to as viruses) are named after the viruses...

  5. What the HEK?

    Type
    Blog Post
    Published
    April 18, 2023, 1:15 p.m.
    ... is present in this line, making it genetically female. HEKs are generally cultured as an adherent monolayer...blog Five Popular Model Organisms 10 Tips for Mammalian Cell Culture Adenoviral Vector Production and...

  6. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...valuable for CRISPR/Cas9 stem cell editing. The small molecule NSDB and Na+ hypertonicity are key elements... Introduction of macromolecules into cultured mammalian cells by osmotic lysis of pinocytic vesicles. ...

  7. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    Published
    April 7, 2022, 1:15 p.m.
    ...generations of iGluSnFR had limitations: they were not optimal for experiments requiring high signal to noise ...fusion to the PDGFR transmembrane domain in the mammalian expression pMinDisplay vector. GPI contains a ...

  8. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    Published
    Feb. 9, 2021, 2:15 p.m.
    ...aside Breakout Rooms to divide the audience into small sessions and do a quick break to chat. This can ... but can also be used to brainstorm on data in smaller groups, where for instance the presence of the ...

  9. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ... Assembly method from New England Biolabs® has minimal upfront requirements and allows you to expediently...generation, green chemistry and understanding the minimal genome. Many of these techniques require more complex...

  10. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    Published
    June 20, 2017, 2:30 p.m.
    ...handling recommendations Not all O-rings function optimally at -80°C. For samples that are shipped on dry ...changes in our solutions are transient and return to normal once the tube is opened and equilibrium with ambient...

  11. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    Published
    March 24, 2016, 2:30 p.m.
    ...PAM-distal side of the break and 91bp on the PAM-proximal side, consistently supported higher frequencies...attained without any of the error-prone repair that normally accompanies Cas9 cutting. We still don’t know ...

  12. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ...yourself to be as productive as you would be in a normal situation. For some other resources on working...Science, or Resources to Engage Kids at Home. It’s normal and okay to feel overwhelmed right now, but hopefully...

  13. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ...examining relationships between microbes and insects, mammals, and plants, among others. I particularly enjoyed...doesn’t love a book series that features a young female protagonist, some clever magic, and a satisfying...

  14. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    Published
    Sept. 12, 2019, 1 p.m.
    ...recently released our serotype testing AAV, which are small (20 ul) samples of EGFP-expressing AAV packaged ...resting fluorescence and can be used for imaging small neuronal processes (dendrites and axons). jGCaMP7c...

  15. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...and has extensive experience targeting RNA with small molecules. Other members’ projects range from hacking...Ph.D. grad student at Boston University studying mammalian immunology, and in his free time, he is pursuing...

  16. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    Published
    Oct. 26, 2017, 1:59 p.m.
    ...plasmids in the library are differently sized, smaller plasmids may be amplified preferentially, skewing...Brunello library is shown in Figure 2. You can use a small PCR product because the plasmids in the library ...

  17. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...alternatives - RNAi and Cas13. CRISPR-Csm doesn’t degrade proximal RNAs nor does it have high levels of off-target...changes of non-target genes and RNA-seq of a nuclear (MALAT1) and cytoplasmic (CKB1) RNA target. Adapted from...

  18. Components of CRISPR/Cas9

    Type
    Blog Post
    Published
    Feb. 2, 2016, 5 p.m.
    ...or closely related Cpf1) to cut the genome and a small RNA (gRNA) to guide this nuclease to a user-defined...used for CRISPR-mediated genome editing, these two small RNAs have been condensed into one RNA sequence known...

  20. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    Published
    July 22, 2025, 1:15 p.m.
    ...Luciferase is the best known: it’s derived from the small (19 kDa) luminous shrimp (Oplophorus gracilirostris... they emit or to optimize their expression in mammalian systems. Our luciferase collection page has a ...
Showing: 381 - 400 of 662 results