We narrowed to 794 results for: Mpl

... had to get used to the workspaces, as well as complete the lab safety and cybersecurity training before...project is a major project given to each intern to complete and present on.    Figure 1: Addgene's...obtain eight clones. We ran digested and undigested samples of the clones on an electrophoresis gel in order...Toj-Villagran is a rising senior in high school who just completed their summer internship at Addgene. They plan ...

...Suitability for in vivo experiments, due to their simple structure    sdAbs in research These advantages...becoming valuable tools for diverse applications. For example, RANbodies can be used as an alternative to conventional...manipulate cellular functions, like in this early example we highlighted back in 2015, where sdAbs were used...around using sdAbs in diagnostic applications. For example, fluorescently and radioactively labeled sdAbs ...

...genetic screens employ various on/off switches to measure the function of a gene. For example, they eliminate...modifying the gene of interest in any way. For example, if green fluorescent protein (GFP) was the reporter...monitors due to transcriptional fusion.   Examples of promoter, enhancer, and gene traps at a genomic...

...bosses and employers. Availability of superiors for consultation, advice and brainstorming. Employees must ...Intelligence or to hone delegation skills, for example. So aside from a focus on removing obstacles, what...organizational decision-making. It is imperative that employees are thanked for their work by the words and actions...

...The technique relies on the basic ability of complementary basepairs adenine (A) and thymine (T) to hybridize...insert comes from using Taq polymerase for the amplification step since Taq polymerase leaves a single deoxyadenosine...deoxyadenosine (A) at the 3' ends of PCR products. The complimentary “T” in the pair comes from a topoisomerase I-linearized... add unique restriction sites on the ends) and amplify your sequence of interest with Taq polymerase using...

...career Developmental biology caught me early on: a simple egg is packed with information. I am fascinated...individual diversity to get from gamete to a visibly complex - and unique - adult. My initial research directions...communication professionals who are thinking about, for example, how to use new technologies to reach the right...the world of scholarly publishing. And see for example the Eighth International Congress on Peer Review...

... students Collaborations may be less “complicated” - for example, easy to initiate a collaboration without...this topic, the authors state, “Both explicit and implicit aspects of today’s postdoctoral training can directly...every 3-6 years—a short and exciting ride Complicated matrix structures are common Small integrated...

...Viruses are simple: a genome packaged in a protein shell (Taylor, 2014). They’re so simple that we can... even decide if they’re alive or not. Yet these simple, small particles have quite the outsized impact... been genetically modified to either limit or completely eliminate their replicative ability. The remaining...

... the collection all the time! We’ll serve up a sampling of some of the most popular and give you a tour...useful for screening for binding properties. For example, several coronavirus libraries have been used to...: Preparing Pooled Libraries Pooled Library Amplifications Resources on Addgene.org Browse all pooled... to Plasmid Pooled Libraries CRISPR Library Amplification Protocol ...

... detection methods. For example, you can provide location, titer dose (Example: 1E13 GC/mL) and subject...this resource for the community. There are a few simple steps you can take to input your data for sharing...and assessment of your experimental results. For example, if you visualized expression after AAV injection...

...research struggles in papers. They recount accomplishments in talks and interviews. They even tell short...Story Telling at work Don’t believe me? Here’s an example of storytelling to answer an informational interview...it worked out for all of us.” This is a classic example of the STAR (Situation, Task, Action, Result) interview...than I’m able to produce. But it’s also a great example of storytelling – you get context, time, cause,...

...repair template plasmid to create fluorescent and 3xFLAG-tag fusion proteins. The repair template and Cas9...The data are clear - the SEC-based system is both simple to use and highly efficient for C. elegans genome...elegans protein. The his-72 locus is shown as an example. Used with permission from the Genetics Society...

...Zalatan JG, et al.'s recent paper, "Engineering Complex Synthetic Transcriptional Programs with CRISPR ... the eukaryotic cell for the execution of many complex cellular behaviors, such as differentiation or ...customizable single guide RNA (sgRNA) that bears complementarity to a 20bp target sequence (Qi et al. 2013)..... (2013). PubMed. Zalatan, J. G. Engineering Complex Synthetic Transcriptional Programs with CRISPR ...

...reason is that I knew that the mind-set of the employer was not such that he/she would be looking at where...have done?” and “Would their academic experience compliment this sales and marketing role?”. Studying for... out more than necessary for my interview. The simplest place I could think of to start was “Marketing...about marketing would be to start reading a broad simple overview, learn some jargon and key phrases and...

...discussion. The Forced Choice Analysis is a fun example and you can find it in our Mentoring for Scientists...on setting SMART goals using a Development Plan template. A Development Plan or mentoring journal will ...help the Mentee feel a sense of accountability to complete action items and make progress. To illustrate ...with regular accountability checks. Focus on implementation of your goals and respect the time your Mentor...

...ul) samples of EGFP-expressing AAV packaged in various serotypes. This collection is now complete! pAAV-CAG-GFP.... Likewise, a lot of scientists have requested samples of AAV for testing purposes. To address this, we... kit with all the serotypes, since these 20 ul samples are distributed individually. See our Serotype ...

...formation. The quest for plant compounds to treat complications of diabetes Gloria Otito Izu, PhD Candidate...nephropathy, one of the major pathophysiological complications of diabetes mellitus. Recombinant human proteins...Now he aims on tracking pollution in rivers by employing synthetic biology resources and microfabrication...robot that will “swim” in the river, take water samples at specific locations, measure fluorescent signals...

...thinner membrane. Procedure The stain is a pretty simple process. First, flood the heat-fixed smear with...or pink, as shown in the image below. An example of Gram positive (purple) and Gram negative (red...is added, it interacts with the CV+, creating a complex within the peptidoglycan layer, staining the cell...the peptidoglycan layer, trapping the large dye complex created by the interaction of CV+ and I- in the...

...genome editing.  For a few reasons, Cpf1 is a simplified system for editing multiple targets compared ...s ability to process its own pre-crRNA arrays simplifies the crRNA cloning process. For cloning, Zetsche...in one direction. See the diagram below for an example of how this works. Make sure to order 5’ phosphorylated...latest developments in CRISPR technologies.  It’s a simple and effective method with multiple applications...

...Anti-FLAG frankenbody (FB) construction from the complementarity determining region of an anti-FLAG scFv and... the tag can recruit multiple frankenbodies to amplify the signal.  Liu et al. Genes Cells. 2021. https...genes within a single cassette provides increased simplicity for combinatorial genetic screens, and this optimized...Penn State Protein Ladder  by: Rachel Leeson This simple, inexpensive plasmid-based ladder system is a cost-effective...