We narrowed to 473 results for: NOL

1. Split Fluorescent Proteins for Studying Protein-Protein Interactions

   Type

   Blog Post

   Published

   May 3, 2022, 1:15 p.m.

   ...Collection Split Luciferase systems, including NanoLuc®, DULIP, LuTHy Other split protein tools: Split-TurboID...

2. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

   Type

   Blog Post

   Published

   Dec. 14, 2021, 2:15 p.m.

   ...repeat and scFv–TET1 catalytic domain fusions. Nat Biotechnol 34, 1060–1065. https://doi.org/10.1038/nbt.3658...

3. Plasmids 101: Modular Cloning

   Type

   Blog Post

   Published

   March 12, 2024, 1:15 p.m.

   ...assembled into many combinations. Even with current technologies, it can be extremely hard to predict how a single...

4. Progress Towards a PAM-Free CRISPR

   Type

   Blog Post

   Published

   March 7, 2024, 2:39 p.m.

   ...genomic landscapes should be editable with CRISPR technology.     Fig. 1: SpCas9 has sparse PAM coverage...

5. Viral Vectors 101: Chemogenetics

   Type

   Blog Post

   Published

   July 11, 2023, 1:15 p.m.

   ...Gi-coupled receptor in transgenic mice. Nature Biotechnology 17:165–169 . https://doi.org/10.1038/6165 Roth...

6. A Look at Addgene's QC Process

   Type

   Blog Post

   Published

   May 6, 2025, 1:15 p.m.

   ...dedicated scientists with expertise in sequencing technologies and bioinformatics. Through their efforts, the...

7. Design Tips for Prime Editing

   Type

   Blog Post

   Published

   Jan. 23, 2025, 2:15 p.m.

   ...pegRNAs improve prime editing efficiency. Nature Biotechnology, 40(3), 402–410. https://doi.org/10.1038/s41587...

8. Making CRISPR Plasmids Using Fragmid

   Type

   Blog Post

   Published

   Aug. 29, 2024, 1:15 p.m.

   ... enables rapid assessment of emerging CRISPR technologies. Cell Genomics, 4(3), 100519. https://doi.org...

9. Synthetic promoter AAVs for cell-type specific expression in retinal cells

   Type

   Blog Post

   Published

   March 12, 2020, 1:15 p.m.

   ...more about precise neuronal targeting using AAV technologies Browse all of our viral vector blog posts Resources...

10. Early Career Researcher Toolbox: Finding Relevant Papers

    Type

    Blog Post

    Published

    Jan. 9, 2020, 1:45 p.m.

    ... contain a short summary of the topic and a chronological list of highly cited papers that contain your...

11. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type

    Blog Post

    Published

    Sept. 13, 2016, 2:30 p.m.

    ...Massachusetts Boston Christine Oslowski, PhD - Biotechnology Communications Specialist at AsisChem Inc. and...

12. 5 Tips for Troubleshooting Viral Transductions

    Type

    Blog Post

    Published

    Aug. 11, 2016, 2:23 p.m.

    ... quantification as measured with branched-DNA technology." Journal of clinical microbiology 37.6 (1999...

13. Plasmids 101: FLEx Vectors

    Type

    Blog Post

    Published

    April 28, 2016, 2:30 p.m.

    ... able to distribute materials containing FLEx technology. Read the genOway press release for more information...

14. Tips for Titering Your Lentiviral Preps

    Type

    Blog Post

    Published

    March 15, 2016, 2:30 p.m.

    ...of lentiviral vector titration methods."BMC biotechnology 6.1 (2006): 1. PubMed PMID: 16836756. PubMed...

15. Illuminating Epigenetics with A FRET Based Biosensor

    Type

    Blog Post

    Published

    Nov. 19, 2015, 3:30 p.m.

    ...reporting methylation status of these sites. This new technology opens up a new way to visualize changes in gene...

16. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

    Type

    Blog Post

    Published

    Oct. 30, 2018, 1:04 p.m.

    ...pluripotency and nuclear reprogramming. Adv Biochem Eng Biotechnol, 2009. 114: p. 185-99. PubMed PMID: 19343304....

17. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type

    Blog Post

    Published

    Oct. 11, 2018, 12:30 p.m.

    ... While T4 DNA Ligase, the mainstay of most biotechnological cloning efforts for over 50 years, prefers...

18. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type

    Blog Post

    Published

    Oct. 12, 2017, 2:26 p.m.

    ...plasmids for protein tagging, including luciferase (NanoLuc), fluorescent proteins (TagGFP2, TagBFP, TagRFP...

19. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type

    Blog Post

    Published

    Sept. 12, 2017, 1:44 p.m.

    ...sequencing capabilities and Seqwell’s plexWell technology, we can now perform NGS at Addgene to sequence...

20. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type

    Blog Post

    Published

    May 4, 2017, 2:30 p.m.

    ...Takeshi, Zhongmei Li, and Michael S. German. "Chronology of islet differentiation revealed by temporal...