We narrowed to 855 results for: NTS;

...They provide accounts of their research struggles in papers. They recount accomplishments in talks and... pay attention. Highlight your lowest points and highest points and make clear what was difficult and ...you don’t like it yourself. Be proud of your achievements and accept and acknowledge your failures. Foster...

...monomeric variants of Clavularia cyan fluorescent protein: identification of the molecular determinants of color...therefore can be used in immunoprecipitation experiments with other fluorescent proteins. Find mNeonGreen... coli with a gene library of approximately 484 variants, including some potential tetramer-breaking mutations...a protein which had a total of 31 amino acid replacements relative to CFP484. Because the excitation and...

...academia, it is especially important for graduate students and postdocs to get a perspective on other types...use of resources and pay attention to budget constraints Academic science is much like industry but the...science’s sake More opportunity to train and teach students Collaborations may be less “complicated” - for...tell you to pick up the pace Constant worry about grants and renewals What is different about non-academia...apple can change entire culture) Specific requirements for hiring more common Likelier to hire on ...

...professional societies hosting virtual networking events. Download Addgene’s Science Career guide to learn...reproducibility best practices Support your future experiments and other researchers by implementing reproducibility...out Reproducibility for Everyone. Plan out new experiments carefully. Consider submitting a registered report...consider using this framework to design your future experiments.  Use Addgene’s AAV Data Hub. You can search ... the AAV Data Hub for tools to use in future experiments. If you’ve used Addgene’s AAV tools, you can ...

...while sipping an afternoon coffee in between experiments. The longer we are in this flexible work schedule...people use is the built-in whiteboard, in which participants can contribute and sketch on the board without...time breaks up the monologue a little and gives participants something else to focus on. Use collaborative... creativity are pivotal in writing publications/grants too. As described by several works (Wuchty et al...be done randomly or manually assigning which participants to group together. Breakout Rooms can be used...

...culture and in live zebrafish. For cell culture experiments, only dL5**-expressing cells treated with MG-...neighboring bystander wild-type cells.   Zebrafish experiments with MG-2I-dL5** were performed with embryos ...Zebrafish are an ideal model organism for these experiments because developing embryos are transparent, so...also show that MG-2I-dL5** only generates small amounts of non-specific ROS when exposed to YFP and RFP...your experiences using photosensitizers in the comments below!   References 1. He, J., Wang, Y., Missinato...

...digested with ribonucleases, ribosomes protect the segments of mRNA to which they are bound. These regions...color switching, TRICK requires the following components: a GFP protein that binds to the coding region...the mRNA to the plasma membrane; this sequence prevents diffusion of the mCherry labeled mRNA and keeps...second. They also explored the effects of 5’ UTR variants in translation of cell cycle protein Emi1, showing...coding sequence and 3’ UTR. Before beginning experiments, it’s important to verify that these changes ...

...Zhou and Fatima Enam. There were many excellent applicants but Xin and Fatima stood out for their creativity...learn more about Xin’s work with photoswitchable components of the ERK signaling pathway in our recent blog...first design is a whole cell biosensor with two components - an enzyme that breaks down the HMO of interest...communication skills throughout her training and wants to make her work understandable to people both inside...Choose the Brightest Fluorescent Protein for Your Experiments Resources on Addgene.org Find Fluorescent Protein...

... production of viruses used to deliver CRISPR components to cells or organisms under study. In the past...a single cell line and proceed to use it for experiments.” - Alex Chavez, researcher at the Wyss Institute...particularly prominent problem in CRISPR genome editing experiments. We aim to alleviate this frustration by producing...primary goal. Getting these quality-controlled reagents from Addgene can greatly expedite a researcher's...our viral services. Please send any questions or comments to [email protected]. Finally we’d like to thank...

... inactive dCas9 molecules. We followed these experiments with more detailed investigations into the release...delivery by nucleofection The in vivo editing experiments in this publication were performed using a technique...well as the sequences of our validated editing reagents can be found on our plasmid pages. We encourage...encourage any labs performing genome editing experiments to try nucleofection as (in our hands) this technique...transfection approaches. Moreover, the cost of reagents is relatively modest, requiring expressed Cas9...

... this system to develop a bacterial camera that prints a chemical image, as well as a genetic method for...experimented with the promoter strength of various components, removing inducible promoters that could crosstalk...increased dynamic range to around 100-fold. These improvements will allow researchers to better fine-tune the... input light intensity. In addition to these improvements, the constructs have been greatly streamlined...larger systems. The work of Schmidl et al. also represents a large step forward in optimizing TCSs. Over...

...does one stand out amongst a pool of successful applicants and get chosen to work in his or her lab of choice...documented history in science. Potential graduate students typically go through a rotation, which reveals...we do fits in with what they want to do. The experiments must fit with their interests.” George Church.... Alternatively, perhaps the lab publishes and presents regularly with labs around the world. You may ...the happy dance at least once in awhile. Acknowledgments Thank you to Dr. Tom Ellis (Imperial College...

...race to find vaccines, therapeutics, and other treatments to stem the spread of COVID-19. That’s why we...you used plasmids from this collection in your experiments and published your data, please fill out this...expression vectors containing various spike protein variants are also available. Plasmids are continually added...proteins, and membrane proteins. Although all viral components are available in bacterial expression plasmids...purification of SARS-CoV-2 proteins for downstream experiments and offer flexibility in tag, cleavage, codon...

...and editing speed and consists of the following components: A plasmid in the host bacterium expressing ...infectious. The editing speed dictates which phage variants can propagate faster than the rate of dilution...uninfected host cell culture). This dilution step represents the major difference between PACE and PANCE: ...stringency PACE, resulted in the evolution of TadA variants with much higher base editing activity compared... TadA-7.10 enzyme. After testing numerous TadA variants, they identified TadA-8e, which edits 590-fold...

...facets of life. It also seems as if many of the experiments in neurobiology (and really in all of biology...minimal immunological effects. AAVs are used in experiments targeting genes to specific cell types in the...us how useful the service has been for their experiments. This helped reinforce our goals of making more...the Addgene blog to get some ideas for future experiments and to find tools that will make your transition...many biological fields the number potential of experiments enabled by physical, software, and biology-based...

...involving protein stability or binding experiments. Potential experiments that one might perform using the ...transcripts for degradation. Despite how easy these experiments are to physically set up in the lab, RNAi is ...the specific gene producing long unspecific RNA fragments that are incorporated into the dsRNA necessary...membranes, organelles, and other subcellular compartments is via proteomics--the study of the distribution...

...controlling cell signaling. Key components of OptoNBs OptoNBs have two key components: an AsLOV2 domain and a ...activity in cells.  Let’s shed some light on the key components of OptoNBs, how they are generated, and their...variable heavy chain antibody domain.  How do these components interact? When fused together to form an OptoNB...below table summarizes the results for these experiments.  AsLOV2 Inertion Site mCherry #1 mCherry ...

...throughput? Will it be sensitive enough? No one wants to spend time screening hits that aren’t “real” ...line possessing a patient mutation to assess how patients may respond to drug treatment. Another example...particular disease as the input population. For these experiments, the goal is to identify manipulations which ...manipulations are assessed, rather than all manipulation events. To put it another way, only cells or organisms...condition that can apply enough pressure to select for variants of interest, though. A selection can take the ...

...implement these improvements. Let us know what you think about the updates in the comments section below... in action). Read on to learn more about the improvements we’ve implemented and how they’ll make it easier...on. Beyond these simple but powerful display improvements, when you click on any plasmid map, you also...checking in at blog.addgene.org for additional enhancements that will further improve the map displays and...

...’ve got you covered. Defining your control requirements To pick the best tool for the job, it’s important...important to know what the job requirements are. Here are the questions you should be asking. What kind ...with the systems discussed above, just with more elements of control. Tet promoters Tetracycline-dependent...promoters are engineered by placing tet response elements upstream of a minimal promoter. These systems ...expression is tunable by titration with different amounts of tetracycline. Tetracycline regulation is reversible...