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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 751 results for: Nes;

Showing: 381 - 400 of 751 results

  1. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ...Vectors (or empty backbones) are frequently used in molecular biology to isolate, multiply, or express...At Addgene, we have a vast collection of empty backbones that have been designed, tested, and published...vector). Check out our complete guide to empty backbones for additional details and guidance for choosing...

  2. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    Published
    April 15, 2014, 3:11 p.m.
    ...discards according to the Cambridge, Massachusetts guidelines and wear an extra sweater so we don’t have to...visualization or isolation, then using only a few lanes or a small portion of the gel.  What a waste?  I... pour over the sides.  These tiny gels and tiny lanes required less agar, less ethidium bromide, less ...

  3. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    Published
    March 9, 2017, 3:40 p.m.
    ...sometimes impossible) timelines—others are usually waiting Work is concentrated to business hours (mostly), ...with peer mentors, to discuss your strengths, weaknesses, interests and passions is another great way ...

  4. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

    Type
    Blog Post
    Published
    Aug. 8, 2019, 1:19 p.m.
    ...20th century, and many iconic papers, including the ones on the structure of the DNA, were not peer-reviewed...submitted paper in addition to the journal-specific guidelines.  Peer-review step 1: The first read At the first...preferably the following morning - as usually deadlines are pressing. During this second read, I pay more...

  5. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    Published
    June 9, 2022, 1:15 p.m.
    ...communication itself, not the communication method, that determines if something is scientific or science communication...defined by any number of characteristics. Common ones include age range, familiarity with the subject,...audience to engage if they so wish. (And let’s be honest: are you more likely to closely read an email sent...

  6. Antibodies 101: Epitope Availability

    Type
    Blog Post
    Published
    Jan. 11, 2022, 2 p.m.
    ...fall into one of two categories: ones induced by the researcher and ones that happen naturally.  Sample ...working with native proteins instead of denatured ones - well, don’t relax just yet. Proteins often change...

  7. What the HEK?

    Type
    Blog Post
    Published
    April 18, 2023, 1:15 p.m.
    ... HEK is it good for?   As one of the older cell lines in the research community, many cellular research...organism. This pipeline is utilized to transduce cell lines for basic science research all the way up to the...HEK is wrong with it?   All cultured human cell lines are susceptible to contamination by mycoplasma and...

  8. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    Published
    Aug. 18, 2023, 4:15 p.m.
    ...coat (and envelope), has a “gutted” genome with genes central to the replication process removed. This... the viral vector, increasing both safety and usefulness of the tool. In fact, most gutted genomes have...vectors to reproduce when experimentally necessary, genes for the required proteins are packaged in separate...

  9. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ... Blog Perform Site Directed Mutagenesis by PCR Use REPLACR Mutatgenesis to Easily Generate Insertion ... be picked and sequenced for potential positive clones. Once you introduce your 3’-end “A” overhang insert...

  10. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2015, 2:30 p.m.
    ... Bioblasts? Plasmagenes? In the 1940s and 50s, scientists were working to understand genetic cytoplasmic... as everything from parasites, to symbionts, to genes and the labels applied to them were vague or contradictory...biology was never the same. Over the next few years genes from different bacterial (and eventually mammalian...

  11. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    Published
    July 7, 2015, 3:36 p.m.
    ...new method uses a selection cassette containing genes for hygromycin resistance and the Roller movement...testing, Dickinson et al. tested this system across 7 genes in parallel; when they injected 60-80 animals per...desired insertions on the first attempt for 6/7 genes. The data are clear - the SEC-based system is both...

  12. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    Published
    Jan. 21, 2014, 3:08 p.m.
    ... Business competitions   Leadership Management Marketing Communication Business models...positions   Market analyses Patent process Business Models Communication Regulatory knowledge ...

  13. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    Published
    May 21, 2019, 12:10 p.m.
    ...reporter that has a large dynamic range and high brightness. The Shu Lab from UCSF designed an improved apoptosis...reporter that has a large dynamic range and high brightness. This reporter achieves approximately 100-fold...the fluorescent protein collection Find empty backbones for fluorescent protein fusions Browse the fluorescent...

  14. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    Published
    Dec. 20, 2018, 1:15 p.m.
    ...scientists and the public together to build consensus guidelines, but there was never a specific timeline for ... not remembered kindly. However, despite many headlines questioning if He’s experiments prove otherwise...genetics this time?” She lives with her partner (in business and life) in an old Girl Scout Cookie factory ...

  15. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    Published
    April 9, 2020, 1:15 p.m.
    ...potential human helpers of SARS-CoV-2 (especially the ones that are druggable) is a key step in our strategy...as a control. For easier portability, all viral genes are flanked by EcoRI and BamHI restriction sites...study - so you might want to be careful with these clones (and help us figure out what is going on there!...

  16. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    Published
    June 23, 2022, 1:15 p.m.
    ...treat AD using CRISPR/Cas9. They are targeting the genes responsible for both early onset and late onset ...diseases In 2013, a team led by Jinsong Li from the Chinese Academy of Sciences found that mice with a dominant...efficiency. In 2022, despite the increasing effectiveness of CRISPR/Cas9, scientists are still looking...

  17. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ... on and off by binding proximal to their target genes. Since gene expression patterns determine everything...genetic and expression control systems to mimic illness on a molecular level. Atlas resource The TF map...from the library to further investigate specific genes and TF isoforms. Due to the size of the collection...

  18. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    Published
    Feb. 9, 2021, 2:15 p.m.
    ...different institutes, disciplines, and cultural backgrounds.” This work outlines the need for clear communication.../doi.org/10.1371/journal.pcbi.0030044 Wuchty S, Jones BF, Uzzi B (2007) The Increasing Dominance of Teams...

  19. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ...directed transcription from a given promoter. To harness the power of light, Christopher Voigt’s lab created...plasmids, with one RR (ompR) encoded chromosomally. Leakiness was also an issue; some promoter activity persisted...with other pathways. They also reduced system leakiness and increased dynamic range to around 100-fold...

  20. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type
    Blog Post
    Published
    Aug. 28, 2014, 3:34 p.m.
    ...is digested with either HindIII and BamHI alone (lanes 4-5), there is a single band of 7.3 kb representing...interpret the bands that you get in your sample lanes. Choose your ladder based on the expected band sizes...supercoiled, relaxed and nicked. If your digest lanes look like your uncut lane then there is something...
Showing: 381 - 400 of 751 results