We narrowed to 459 results for: PAC;

1. Plasmids 101: Methylation and Restriction Enzymes

   Type

   Blog Post

   Published

   June 30, 2016, 2:30 p.m.

   ... the adenine in AACNNNNNNGTGC or GCACNNNNNNGTT Impact on cloning and digests Although not all prokaryotic...

2. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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   Blog Post

   Published

   Feb. 24, 2015, 7:50 p.m.

   ...gain-of-function screening when combined with a compact pooled sgRNA library. Our SAM-mediated screens ...

3. Antibodies 101: Fab Fragments

   Type

   Blog Post

   Published

   Sept. 5, 2024, 1:15 p.m.

   ...sometimes you want a tool that is a little more compact — more of an antibody fragment rather than the ...

4. Antibodies 101: Conjugation

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   Blog Post

   Published

   March 26, 2024, 1:15 p.m.

   ...region or even just nearby, then conjugation could impact the antibody’s antigen recognition and affinity...

5. Plasmids 101: The protein expression toolbox

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   Blog Post

   Published

   Feb. 16, 2023, 7:05 p.m.

   ...choice cDNA. Both lentivirus and retrovirus can package cDNAs for genomic delivery and have many therapeutic...

6. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

   Type

   Blog Post

   Published

   May 11, 2021, 1:15 p.m.

   ... from these studies is that the tag protein can impact the expression of the transgene of interest. In...

7. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

   Type

   Blog Post

   Published

   Jan. 11, 2017, 3:30 p.m.

   ...two-postdoc households. To better assess the potential impact of these salary fluctuations, we are continuing...

8. Lighting Up Cell Signaling with Photoswitchable Kinases

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   Blog Post

   Published

   July 25, 2017, 1:20 p.m.

   ...signal strength, and how long their signals last all impact transduction of the message. To study signaling...

9. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

   Type

   Blog Post

   Published

   June 15, 2017, 2:30 p.m.

   ... the aforementioned methods only determine the capacity of homodimerization in aqueous solution. In cyto...

10. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type

    Blog Post

    Published

    Nov. 3, 2020, 2:15 p.m.

    ...eliminates the need for intracranial injections. When packaged in PHP.eB, ChRgers and ChR2(H134R) both reach ...

11. Summer SciComm Series: Cognitive Load

    Type

    Blog Post

    Published

    June 16, 2022, 1:15 p.m.

    ...communicate. It’s okay (and encouraged) to leave blank spaces or to gloss over some aspects when needed. The ...

12. New Tool for Lineage Tracing: The ClonTracer Library

    Type

    Blog Post

    Published

    Sept. 22, 2015, 2:30 p.m.

    ...their own work. She says, “I spend a lot of time packing, shipping, and sharing protocols one by one,” and...

13. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type

    Blog Post

    Published

    May 5, 2015, 3:54 p.m.

    ... – F1000 just announced the launch of their F1000Workspace.  Give your favorite searching, reading and...

14. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type

    Blog Post

    Published

    April 22, 2015, 2:06 p.m.

    .... The Apollo 11 mission circuitry that took a spaceship to the moon was built on NOR gates. It’s a fundamental...

15. Working with Nuclear Receptors

    Type

    Blog Post

    Published

    July 15, 2014, 4:03 p.m.

    ...cascades conceivably allows the genome to sense the impact of the total environment on the cell. The NURSA...

16. A Look at Addgene's QC Process

    Type

    Blog Post

    Published

    May 6, 2025, 1:15 p.m.

    ...discrepancy in the plasmid, and that it is not known to impact the function of the plasmid. If the issue is likely...

17. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type

    Blog Post

    Published

    Nov. 14, 2024, 2:15 p.m.

    ...start site function would also help to reveal the impacts of a lncRNA on nearby genes and its role in gene...

18. CRISPR in the Clinic

    Type

    Blog Post

    Published

    Nov. 2, 2023, 1:15 p.m.

    ...Clinical trials are underway to explore CRISPR’s capacity for both classes of edits in cancer therapy. Blood...

19. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type

    Blog Post

    Published

    July 12, 2021, 1:15 p.m.

    ... are cloned into pLVX vectors (for lentiviral packaging) and contain an IRES-Puro marker for stable cell...

20. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type

    Blog Post

    Published

    April 6, 2021, 12:30 p.m.

    ...number one was to ensure that this changeover had no impact on our ability to provide requesting scientists...