We narrowed to 802 results for: Por

...work was with TALENs, in collaboration with the Porteus lab [2]. With the advent of CRISPR/Cas9, we began...correlative biochemical characteristics and ectopic reporter assays. We have been interested in exploring the...transfect CRISPR plasmids into MEL cells by electroporation, sort your transfected cells via FACS, primer...

..., the Addgene board members, vendors, Addgene supporters, and of course, Executive Director, Joanne Kamens...the Addgene family Perhaps the most heartfelt portion of the entire night was when Melina and Ken called... recognized. John and Stephanie Fan have been supporting Addgene from the very beginning and have played...

...had certainly realized the importance of seeking out mentoring as an important aspect to their ongoing success...takes us a decade or more for just the “training” portion of our careers. In that time we may have only 2...

...nonprofit organizations, we have always sought to support scientists at all research institutions. In 2012...includes over 600 plasmids such as luciferase reporters for gene expression analysis and tools for tracking... at [email protected] and we’d be happy to help support you in this process.  ...

...a scientist in training can adopt to ensure opportunities in the future. Studies show that the majority...ignore this in their cover letters. It is also important to express interest, even excitement, in a position... to highlight different skills and to get the important information in the right place on the page. 5)...

...contribution of students in their labs and the importance of the strong, cross-disciplinary collaboration...Prize. What did Dr. Doudna think was the most important part of their work? First, the discovery of a ... research in the scientific community and the importance of this type of experimentation to the eventual...

...Cepko 1. Work hard. There will be periods when opportunities arise. “Grab it!” 2. Don’t get caught up in ... to deadlines, writing up clear and attractive reports, being good at presentations and of course, networking...from heart and hustle. Each PI emphasizes the importance of passion, hard work, collaboration, and staying...

...degree is a means to gain employment, so it’s important to make sure a masters helps you get a job you...from the bench, look for “technical or product support specialist,” “field application scientist,” or ... career ladder is larger and there are more opportunities for promotion. It’s not uncommon for bachelor...

...unpublished materials. What about preprints? Addgene supports preprints and encourages authors to share their...are both excellent ways to provide transparency, support reproducibility, and accelerate science. We encourage... as soon as the preprint is posted in order to support these goals. And we can update your plasmids’ citation...

...surprising, and important, findings being shared. For instance, recently Cellosaurus received a report that HT...cross-references with cell line catalogs and collections. Importantly, Cellosaurus also identifies problematic cell...

...components that make up a plasmid and why each is important. Our “Plasmids 101” series designed to educate...and selectively grown on antibiotic plates.  Importantly, because the bacteria from which plasmids are...expression plasmids, gene knock-down plasmids, reporter plasmids, viral plasmids, and genome engineering...

...worldwide and the presentation made it clear how important it is for us to improve our resources for this...Needless to say we all got very good at applying temporary tattoos on the spot with our trusty spray bottles...microbiology community #ASMMicrobe2016 was a great opportunity for Addgene to let microbiologists know about...

...therapy - avoiding the use of viruses is also important for social and regulatory reasons. 1.) The transposon...puromycin, hygromycin) and fluorescent protein reporters (GFP, BFP, RFP, no FP). The vectors each contain...region of the RPL41 gene, to drive the selection/reporter parts of each construct. This new tool kit allows...

...” instead of levels because it emphasizes the importance of these very different roles. At any stage in...doing, managing or leading. All three roles are important to accomplishing any goal or finishing any project... or highlighting criticisms. To be given the opportunity to take on the role of leader, one needs to take...

...attendees an amazing opportunity to share their cutting edge science in increasingly important field.  Interested...through TALENs and CRISPR-Cas nucleases, provided important context for the rest of the day. He was followed...

...probes, such as antigen-binding fragments (Fabs) (Porter 1959) and single-chain variable fragments (scFvs...of antibody-combining sites within the variable portions of heavy and light chains. Proceedings of the ... PMID:4560694. PubMed Central PMCID: PMC427010. Porter, R. (1959). The hydrolysis of rabbit y-globulin...

...(Addgene #104102 - #104105) using in utero electroporation or with AAVs (prepared with Addgene #104110...Figure 3) that can be introduced using in utero electroporation. Then at your preferred time points, sacrifice...with an index-optimized clearing agent." Cell reports14.11 (2016): 2718-2732. PubMed PMID: 26972009. Ke...

...new tools and protocols that they can easily incorporate into their own labs. This week we are pleased...following sucrose treatment. Moreover, several reports have shown that cells treated with sucrose or other...test this approach with different capsids. Most importantly, because our AAV yields are improved we will ...

... RNA templates to DNA but suffer from several important shortcomings including low processivity (ie. the...29109157. Nanobody toolkit to monitor retrograde transport at the cell surface Article contributed by Marcy...functionalized nanobodies to study retrograde transport from the cell surface. Their toolkit includes ...

...stylistic consistency. If you’ve ever doubted the importance of copy editors, the New Yorker’s “Comma Queen...review were discussed. Science communication opportunities If you are interested in the science communications...summer program that gives graduate students an opportunity to work in radio, print journalism, and other...