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TypeBlog PostPublishedNov. 18, 2021, 2:15 p.m....read more content related to CRISPR technology, subscribe to our CRISPR blog content. In this post we ...
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Antibodies 101: Polyclonal Antibodies
TypeBlog PostPublishedJuly 14, 2021, 1:15 p.m....protein G each have different affinities for IgG subtypes from different animals so it’s important to choose... -
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog PostPublishedMay 11, 2021, 1:15 p.m....the same cell, but not necessarily at the same subcellular compartment as the opsin or chemogenetic receptor... -
Antibodies 101: Fab Fragments
TypeBlog PostPublishedSept. 5, 2024, 1:15 p.m....flexibility of and demand for Fab fragments and subsequently Fab fragments intended for use as primary antibodies... -
Lighting Up Cell Signaling with Photoswitchable Kinases
TypeBlog PostPublishedJuly 25, 2017, 1:20 p.m....few such methods exist and they only work for a subset of kinases. The Problem: There is no common method... -
Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages
TypeBlog PostPublishedJan. 11, 2017, 3:30 p.m....them to keep raising salaries and that we are submitting Freedom of Information Act (FOIA) requests for... -
All in a Twist: dsRNA
TypeBlog PostPublishedFeb. 2, 2023, 2:15 p.m....intermediates, with each one acting on specific substrates and in distinct cell cycles. When RNA:DNA hybrids... -
Antibodies 101: Conjugation
TypeBlog PostPublishedMarch 26, 2024, 1:15 p.m.... (HRP) and Alkaline Phosphatase (AP). Various substrates for each of these enzymes allow you to detect... -
Plasmids 101: CcdB - The Toxic Key to Efficient Cloning
TypeBlog PostPublishedDec. 8, 2016, 3:30 p.m....offer your comments and suggestions, and even to submit a guest blog entry of your own! References 1. ... -
It’s Bioelectric! An Exciting Interdisciplinary Field of Research
TypeBlog PostPublishedOct. 29, 2019, 2:52 p.m....during regeneration, the worm creates two heads. Subsequent cutting results in a two-headed worm, despite... -
Gendered Innovations: Why Does Sex of the Cell Matter?
TypeBlog PostPublishedJuly 21, 2016, 2:30 p.m.... research, they worry about needing more study subjects and control groups. The important point, however... -
Addgene's Tips for Plasmid Quality Control
TypeBlog PostPublishedJan. 14, 2016, 3:30 p.m....to select the appropriate service and read the submission instructions. As for the analysis of a large ... -
Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9
TypeBlog PostPublishedDec. 16, 2015, 3:30 p.m.... Slaymaker et al. made a variety of alanine substitutions throughout the groove in 32 separate Cas9 mutants... -
New Tool for Lineage Tracing: The ClonTracer Library
TypeBlog PostPublishedSept. 22, 2015, 2:30 p.m....cells that represent the preexisting resistant subpopulation 2. Resistant cells among the separate replicates... -
CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt
TypeBlog PostPublishedApril 22, 2015, 2:06 p.m....toolboxes and making them available. We’ll also be submitting parts from another paper for resource allocation... -
Career Coaching for Scientists: Why and Where Do I Find One?
TypeBlog PostPublishedJan. 29, 2015, 1:25 p.m....Additional Resources on the Addgene Blog 6 Steps to Submitting a Resume That Gets Seen Guide Your PhD with ... -
How to Prepare for an Industry Interview
TypeBlog PostPublishedJuly 9, 2020, 1:15 p.m....Tips for writing a good cover letter 6 Steps to Submitting a Resume That Gets Seen Resume writing tips for... -
Engaging with science and society at pgEd
TypeBlog PostPublishedSept. 20, 2018, 12:17 p.m....science-outreach organizations or student publications, submitting op-eds to your local newspapers, or starting ... -
Educational Planarian Tools for Teaching Developmental Biology Techniques
TypeBlog PostPublishedMay 24, 2018, 1:20 p.m....natively expressed mRNAs.These native mRNAs are subsequently destroyed by Argonaute-catalyzed nuclease activity... -
Adeno Associated Virus (AAV) for Cell and Gene Therapy
TypeBlog PostPublishedNov. 7, 2017, 1:59 p.m....untreatable until 2008. Following a single dose of subretinal administration of rAAV2 carrying the human gene...