...in the United States but around the world. As an organization that directly collaborates with NIH-funded... are constrained. This collaborative approach eliminates redundant work, saving time and allowing precious...

... develop tests for food products as opposed to therapeutics 15:15 - 16:26 : Means for testing for pathogens...how It works 18:02 - 19:56 : Why Sample 6's first test was for Lysteria 19:56 - 24:41 : Who is using Sample...

...to activate gene transcription. The researchers tested a variety of combinations to optimize both fusion...500nm). By fusing the two pdDronpa domains to the sites flanking the DNA-binding cleft of Cas9, the fluorescent...Upon light activation, dCas9 is released within minutes and red foci formed 20-40 min later. The CASANOVA...synthetase, read this article). The group first tested photocaging various lysines in Cas9 to determine...

...enriched our repository with 15,020 new plasmids — a testament to the scientific community's commitment to open...brings us closer to making an impact on humanity's greatest challenges. Thank you for being part of this incredible...future of science. Want to learn more about our latest developments? Subscribe to the Addgene newsletter...

...environmental impact of those processes? Who even regulates them? You may be surprised to learn that it hasn... been 50 years since the creation of the United States Environmental Protection Agency, which started ...scientists washed and reused materials such as glass pipettes or inoculation loops. While single-use is now ...

...So, as 2013 comes to a close, let’s take a few minutes to reflect on some of the most noteworthy developments...database to gather the results of Myriad's genetic tests for breast and ovarian cancer risk directly from...granted via a public repository or authors’ own web sites. 9)      The year 2013 has also been the year of...

... was pumpkin. Everyone gets to vote for their favorites, and trophies are awarded and coveted. I’ve only...we head into Thanksgiving weekend in the United States, I look forward to gathering around a large table...you have any traditions that you share with your labmates, be they food related or not? We would love to...

..., M.W., Nguyen, T.H., Coxe, M.A., Miller, B.C., Yates, K.B., Gillis, J.E., Sen, D.R., Gaudiano, E.F., ..., Haining, W.N., & Sharpe, A.H. (2019). PTPN2 regulates the generation of exhausted CD8+ T cell subpopulations...0480-4 LaFleur, M. W., Nguyen, T. H., Coxe, M. A., Yates, K. B., Trombley, J. D., Weiss, S. A., Brown, F....

...for scientific titles generated from the United States Standard Occupational Classification System  and...female to male scientific authorship in the United States (0.428). 4. The #1 (re)tweeted domain by scientists...scientists was Instagram Social media and news sites were also popular. Perhaps scientists, like nonscientists...

...transcriptional regulators. In this system, Cas9 generates a double-stranded DNA break at the genomic loci... the universal sgRNA is not predicted to target sites in the human, pig, or zebrafish genome, this system... Using the structure of CasX at eight different states of assembly from cryo-electron microscopy, the ...

...to make it more accessible. Other accessibility updates include a printer-friendly design and alt text ...on all images. We have also made accessibility updates to our website, which receives 3.8 million visitors...perspective on Addgene through her LinkedIn page. She invites you to connect with her there. More on the Addgene...

...months of strategizing, designing, developing, testing - and repeat - we’re proud and excited to finally...homepage While users are increasingly accessing websites other than through the ‘front door,’ the homepage...Additionally we also wanted to highlight our blog, daily updates, and offer better exploration throughout the site...

...sensitive restriction enzyme DpnI to remove the templates. Digestion products are then transformed directly...that the exonuclease activity of the polymerase generates sticky ends in the PCR reactions. These sticky...time to completely digest the template DNA. Note—templates must have been isolated from methylation competent...

... organs located deep in the body, like the gastrointestinal tract. Require Administration of Substrate...coli aided in ultrasound imaging of the mouse gastrointestinal tract. The E. coli strain Nissle 197 (ECN)...probiotic strain that can colonize the mammalian intestinal tract and has been used for 100 years to clinically...

... may appear to be binding at particular genomic sites when in fact they are just non-specific or indirectly... MNase. Once MNase has been localized to target sites, the nuclease is briefly activated to digest the... an excellent additional measure of DNA binding sites in combination with existing ChIP-seq data sets,...

...used in optogenetic studies are not native to vertebrates and are commonly introduced in zebrafish through... a neural circuit involved in locomotion that generates periodic motor commands for rhythmic movements...  Find plasmids from the Isacoff lab! They then tested what kind of swimming behavior, spontaneous swim...

...breakthroughs often occur when the unexpected infiltrates the realm of focused exploration. Here, we delve...your project's potential. Widening your search creates fertile ground for serendipitous encounters that...beneficial to carry a notebook at all times (or use the notes app on your phone) to capture fleeting thoughts ...

...bacteria, but also exist naturally in archaea and eukaryotes such as yeast and plants. In nature, plasmids...segment of DNA which contains several restriction sites allowing for the easy insertion of DNA. In expression...bacterial cells and selectively grown on antibiotic plates.  Importantly, because the bacteria from which ...

...immune response against a pathogen. The Fc domain creates a link between the antibody and other parts of ...opsonization of IgG and IgM antibodies on a pathogen activates the immune system's classical complement pathway.... (2008). The immunoglobulin constant region contributes to affinity and specificity. Trends in Immunology...

...production on a protocol video. Don't worry - no pipettes were harmed in the making of this photo!  Here... The sense of trust and comfort I had with my teammates acted as my safety net and trampoline all at once... I really believe that the culture at Addgene cultivates trust between coworkers and that trust then translated...