We narrowed to 435 results for: Trac

1. Your Lentiviral Plasmid FAQs Answered

   Type

   Blog Post

   Published

   April 23, 2014, 1:08 p.m.

   ...screening with CRISPR and Lentivirus Read about the ClonTracr Library Resources on Addgene.org Addgene's most...

2. Transferable Skills Guide: External Collaborations

   Type

   Blog Post

   Published

   April 19, 2018, 1:25 p.m.

   ...being clear about what was expected, and keeping track of speakers. During your graduate or postdoctoral...

3. Antibodies 101: Reading a Flow Plot

   Type

   Blog Post

   Published

   Feb. 6, 2024, 3:13 p.m.

   ...., Pons, J. A., & Muro, M. (2018). In vitro intracellular IFNγ, IL-17 and IL-10 producing T cells correlates...

4. The PAM Requirement and Expanding CRISPR Beyond SpCas9

   Type

   Blog Post

   Published

   Aug. 20, 2020, 2:30 p.m.

   ...When choosing a Cas9, remember to check that your tracrRNA and crRNA (or synthetic gRNA) are derived from...

5. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

   Type

   Blog Post

   Published

   Feb. 18, 2020, 2:15 p.m.

   ...people within similar fields (ASM Microbe has eight tracks), something we didn’t have enough people to do ...

6. An Inside Look into Addgene's Dev Team

   Type

   Blog Post

   Published

   Jan. 23, 2020, 2:45 p.m.

   ...Addgenies, development includes everything from tracking plasmids from the moment a deposit kit is sent...

7. Bioinformatics at Addgene

   Type

   Blog Post

   Published

   July 14, 2022, 1:15 p.m.

   ...data for our DNA-based reagents. Not only do we extract as much information as possible from depositing...

8. Antibodies 101: Immunoprecipitation

   Type

   Blog Post

   Published

   Dec. 7, 2021, 2:15 p.m.

   ...strengths.    . Steps of an IP: 1) Protein extracts from cells or tissues contain a complex mix of...

9. CRISPR-based Genome Editing Systems For Editing in E. coli

   Type

   Blog Post

   Published

   April 13, 2021, 1:15 p.m.

   ... plasmids for multicopy integration. (B) Anhydrotetracycline (aTc) induces expression transposases leading...

10. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

    Type

    Blog Post

    Published

    July 29, 2025, 1:15 p.m.

    ...Confirmation Pick 2–3 positive colonies, grow them, and extract plasmids. Confirm the correct guide insertion by...

11. QC Sequencing Technologies at Addgene

    Type

    Blog Post

    Published

    July 15, 2025, 1:15 p.m.

    ...the terminating nucleotide and records it in a “trace” file to determine the sequence. Figure 1...

12. CRISPR 101: Any Base Transversion Editors

    Type

    Blog Post

    Published

    April 1, 2025, 1:15 p.m.

    ...Glossary Here’s a quick refresher if you ever lose track of which acronym is which. Acronym Full name ...

13. Hot Plasmids: Fall 2024

    Type

    Blog Post

    Published

    Nov. 21, 2024, 1:30 p.m.

    ...with Cas9 on an integrative plasmid containing tetracycline repressor TetR and resistance selection marker...

14. How to Write a Scientific Review Article

    Type

    Blog Post

    Published

    Feb. 16, 2017, 3:30 p.m.

    ...This is normal and it means you’re on the right track!  Get feedback After you’re done editing, send it...

15. Mesothelioma - Causes, Symptoms, and Treatment

    Type

    Blog Post

    Published

    Dec. 13, 2016, 3:30 p.m.

    ...Addgene's Cancer Collection Pages Learn How the ClonTracer Library Can be Used to Study Cancer Drug Resistance...

16. Antibiotic Resistance: An Old Solution but a New Problem

    Type

    Blog Post

    Published

    Nov. 10, 2016, 3:30 p.m.

    ...today, but it doesn’t have to be some looming, abstract, convoluted scientific concept. It is imperative...

17. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type

    Blog Post

    Published

    July 23, 2019, 12:46 p.m.

    ...in vivo transduction of AAV serotypes generally tracks between strains of mice. This suggests that a small...

18. Designing Your Chalk Talk for the Academic Job Interview

    Type

    Blog Post

    Published

    March 12, 2019, 1:37 p.m.

    ... by your presentation, then you’re on the right track. Furthermore, practice getting back to your story...

19. Plasmids 101: Codon usage bias

    Type

    Blog Post

    Published

    Sept. 27, 2018, 1:09 p.m.

    ...properly. For example Pechmann and Frydman found that tracts of non-optimal codons are associated with specific...

20. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type

    Blog Post

    Published

    Oct. 19, 2017, 1:54 p.m.

    ...proteins needed for later experiments (cellular tracking for instance) can be controlled with FLP-FRT. ...