We narrowed to 530 results for: abo.2

... and whether I would be able to carry out a yeast-2-hybrid library screen; instead I thought they would...for Dummies”. The fastest way to learn something about marketing would be to start reading a broad simple...introduction to the financial world. It teaches you about selling products which is an important part of the...Seán on twitter @Macfhearraigh.    Read more posts about Scientific Career Development ...

... ligands can elicit varying degrees of β-arrestin-2 recruitment to receptors, indicating their agonistic...small-molecule fluorescent labels. Nature Methods, 19(2), 149–158. https://doi.org/10.1038/s41592-021-01338... A., Purkayastha, N., Flad, K., Schmidt, M. F., Tabor, A., Gmeiner, P., Hübner, H., & Weikert, D. (2020...

...is the most charged, with 5 negatively charged and 2 positively charged amino acids. FLAG tag has been ...Tags have a lot of major benefits – as discussed above – but there are a few instances when they should... hindrances, and destabilization can be brought about by the tag so it’s important to validate tagged ...

...our SPARC blog post for more details. Figure 2: Dense (c), intermediate (d), and sparse (e) labeling... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.  ...non-overlapping cells. Until now, tools for doing this were labor-intensive and required using manipulations such ...

...containing complementary polynucleotide chains. J Virol 9(2): 394-396. Lecomte E, Tournaire B, Cogné B, Dupont...share the procedures and Python scripts with laboratories and viral vector cores routinely producing AAV...on Addgene.org Find AAVs at Addgene Learn more about Addgene's viral service Find protocols for working...

...Bacterial Cell Envelope. Cold Spring Harb Perspect Biol. 2(5) a000414 (2010). Cleveland Clinic. Gram Stain. https... it can be used in a lab? Here we’ll be talking about the ins and outs of this classification system, ...The classification system is also useful in a laboratory setting. When designing a plasmid to be inserted...

...delivering an AAV construct expressing channelrhodopsin 2 (ChR2), a blue light sensitive cation channel, fused...I often wonder about the gut-brain axis (admittedly for self-serving reasons – I want to understand my...

...extraction protocol for more details.   Figure 2: An uncut plasmid is expected to form 3 bands: nicked...molecular biology and viral vector protocols Learn about the common lab E. coli strains Find tips for viral...

...structure-based framework engineering. Methods, 34(2), 184–199. PubMed PMID: 15312672. Inbar, D., Hochman...Additional resources on the Addgene blog Learn about visualizing protein turnover Download the fluorescent...

...yourself greater chances for success.  Build a team of 2 or 3 Don’t go it alone.  You’ll find that no matter...spring of 2015 and since that time have received about 3 project proposals per month.  We are currently...

...aspects of the work you presented may also be unclear. 2. Make the audience your primary focus When writing...  If someone asks a question about something they did not understand about your presentation, question ...important and relevant to them.   When you think about it, virtually every milestone in a scientist's career...scientists learn from advisers and other mentors by collaborating as they craft sentences for abstracts and presentation...  If someone suggests a change to a slide, think about whether the same principle applies to other slides...much bigger job than merely telling your audience about the work you've done. If you want to maximize the...expert audiences in other fields, like when collaborating with colleagues in other fields or writing grant...

...to inspire and open a new conversation about GMOs.  Challenge #2: Technical obstacles to plant synthetic...Revolution Bioengineering. Read their previous blog post about how they started their company here. The first transgenic... foundation of life on earth, and opportunities abound to build better fuels, feeds, foods, and fibers...not understand, and the complicated and unique metabolic processes of plants are being investigated on ...biochemical pathways involved in central anthocyanin metabolism are well understood. Additional work has also...knockouts to better understand developmental, metabolic, and photosynthetic processes. Often the tools...the color-changing flower, and we’d love to hear about the obstacles you’ve faced as a plant scientist,...

...perceived climate change risks. Nature Climate Change, 2(10), 732-735. doi:10.1038/nclimate1547 Kunda, Z. (...discussions about the pros and cons of different fabrics while we worked. We also had discussions about the difference...grad student. I chose to focus on misinformation about sexual and reproductive health, as the stigma around... misinformation can flourish. I’ve never forgot about all the anxious, whispered questions from friends...the study design or by their preconceived ideas about the controversial medical issues. Participants ...none of the participants had preconceived opinions about “adeolitis.” The participants in my study were ...make reusable cloth menstrual pads while we talked about the environmental impact of disposable products....

...create a recombinant antibody.     Figure 2: Changes to the heavy chain or light chain genes within... Learn more about research applications for antibodies in Intro to Antibodies Read about the Developmental...antibodies in your experiments, you may be curious about all of the different kinds of antibodies that are...Disadvantages of monoclonal antibodies As mentioned above, genetic drift is one concern when using hybridomas...Hybridoma Bank, a repository for hybridomas  Learn about monoclonal antibodies for immunotherapy Resource...

...and in line with Addgene style.        Fig. 2: Reference image (left) provided to our graphic designer...We also want to tell you about things you didn’t know you wanted to know about, like research breakthroughs... want to know more about! You can also suggest blog topics or even reach out about writing a guest post...technical skills, and experiences from thousands of laboratories that makes up our Help Center, blog, website...

...axon terminals to the soma (retrograde tracer) (Fig. 2). Viruses as neuronal scalpels Viruses can be injected... Get Started with AAV Learn more about Lentivirus Find out More about Addgene's Viral Service Resources...We often think about the brain’s function in terms of its regions. But equally important is the way the...head bone’s connected to the neck bone,” but with about a hundred billion neurons and six hundred trillion...

...improve the brightness of previous Nano-lanterns by 2-4 fold and permit five-color fluorescent imaging! ...Since their first publication in 2012, the Nagai laboratory has assembled a collection of multicolored nano-lanterns...Nano-lanterns, Takai et al. continuously imaged cells for about 4 hours! The latest enhanced Nano-lanterns (eNL)... Nano-lanterns and your experiments The Nagai laboratory has a done a wonderful job characterizing Nano-lanterns...Additional Resources on the Addgene Blog Learn about Photoactivatable Fluorescent Proteins Check Out ...

...to visualize multiprotein complexes.  Fig. 2. Multiplex immunolabeling with subclass-switched recombinant... called NABOR (Neuroscience AntiBody Open Resource). The antibodies distributed through NABOR are recombinant...troubleshooting methodologies drew me to learn more about the process that was used to create this important...secondary antibodies are commonly used in research laboratories to detect the different subclasses, but there...

...work with Grows extremely fast (doubling time of ~ 2 hours) Amenable to genetic manipulation The first...pombe have dominated the research scene. But what about the other yeasts – in the biomedical world, are ...pharmaceutical companies (along with some research laboratories) have picked up the system for some of the uses...eukaryotic cells and peroxisomes are where methanol metabolism occurs in methylotrophic organisms. K. phaffii...

...Without binding to the guide RNA, Cas9 cannot cut. 2. Bind to target DNA in the presence of a guide RNA...that you know about all the different components of CRISPR, It's time to start thinking about how you can...Use the following flow chart to get you thinking about the types of experiments you're planning on doing...