We narrowed to 807 results for: app
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TypeBlog PostPublishedNov. 14, 2016, 3:30 p.m....been requested 100+ times Since we so sincerely appreciate our depositor’s dedication to scientific sharing...plasmids with us. One way we can gauge if people are happy depositors is if they deposit plasmids more than...off! To share your photo, tag @Addgene and #happydepositor on Twitter. All Blue Flames started with a ...
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Addgene at the ASM Microbe 2016 Conference
TypeBlog PostPublishedJuly 7, 2016, 2:30 p.m....as #happydepositors who stopped by to say hello. Needless to say we all got very good at applying temporary...six continents who dropped by the booth. We were happy to chat with lots of researchers who weren’t aware... -
Sleeping Beauty Awakens for Genome Engineering
TypeBlog PostPublishedJune 30, 2015, 2 p.m.... though not all, of these defenses. For some applications of genome engineering - such as certain forms...and improved SB transposase, SB100X, that is approximately 100-fold more efficient than the first-generation...Epub 2009 May 3. PubMed. 3. Singh H, et al. A new approach to gene therapy using Sleeping Beauty to genetically... -
Management for Scientists: What Makes a Good Manager Anyway?
TypeBlog PostPublishedDec. 9, 2014, 2:22 p.m....team. It is pretty clear that if people are mostly happy at work, they will work harder and contribute more...contribute to job satisfaction: A feeling of being appreciated and valued This often stems from being included... Need feedback on their work but prefer to be approached as peers rather than subordinates Need mental... -
The Stingy Scientist: How the Baby Gel Box Was Born
TypeBlog PostPublishedApril 15, 2014, 3:11 p.m.... supplies every 10 years or so. Actually, I was happy to hear that I am not the only Addgenie who has ...I started looking for smaller and smaller gel apparatus until I settled on using glass slides. With just...this one only cost a few dollars. I used this apparatus for 15 years before I hung up my pipets. ... -
5 Uncommon Ways to Gain Career Skills During an Uncommon Time
TypeBlog PostPublishedOct. 20, 2020, 1:15 p.m....chatting makes it pretty easy to make 30 minute appointments with people all over the world. Think about ...are many different kinds of grants that you can apply for. If you are in a lab, your PI is likely working...working on a number of them. Find one to apply to or offer to help with lab grant writing. You may not get... -
Science Careers: Unruly Interests Feed Many Paths
TypeBlog PostPublishedSept. 26, 2017, 12:12 p.m....Science magazine. That one I just could not resist applying for. Not that I knew anything about being an editor...constellation of interests and skills than what fits for applied research. I focus in general terms on a couple ...Review and Scientific Publication, where diverse approaches to peer review were discussed. Science communication... -
In Vivo Biotinylation of Bacterial Fusion Proteins
TypeBlog PostPublishedJan. 25, 2018, 2:09 p.m....of an experimental protein-ligand interaction approaches that of GST-glutathione. Biotin-based immobilization...lack of site selectivity typically diminishes the apparent affinity for the ligand, especially if the modified...located near the ligand binding site. A selective approach entails the use of the enzyme biotin ligase, which... -
Addgene Begins Distribution of Recombinant Antibodies
TypeBlog PostPublishedMarch 28, 2022, 4:30 p.m....community about how the antibodies work in various applications, enabling you to better plan your experiments...the target antigen, and performance in various applications are included in the full webpage. Educational...recombinant antibody production as well as antibody applications. In addition, basic and advanced affinity reagents... -
Celebrate Open Data Day with Addgene’s AAV Data Hub!
TypeBlog PostPublishedMarch 5, 2020, 2:15 p.m....form has 5 general question types and it takes approximately 15 minutes or less if you have all of your data...under copyright, you will need to obtain written approval from the copyright owner and will have the opportunity...viewing the data you have any feedback at all, we’d appreciate you sharing it through this form. In honor of... -
New and Upcoming Viral Vectors - December 2019
TypeBlog PostPublishedDec. 19, 2019, 2:40 p.m....different levels of apparent dopamine affinity, with 113050 offering high apparent affinity, and 113049...113049 offering medium apparent affinity. Plasmid Serotype Name 113050 AAV9, AAVrg pAAV-hSyn-GRAB_DA1h... -
10 Great Guest Posts We're Thankful For
TypeBlog PostPublishedNov. 18, 2016, 3 p.m....artefacts in your experiments. Read this post from Erik Snapp, Associate Professor at Albert Einstein College ... membranes. Read the full post for potential applications of R-bodies and to find a video of them in action... to your DNA sequence of interest. Potential applications of the system include activation and repression... -
Advice for Moving Into Sales After Your Science Postdoc
TypeBlog PostPublishedAug. 21, 2014, 3:15 p.m....on the marketing or business background of the applicant. However, although this position did look for ...experience or a marketing interest that would be able to apply themselves immediately to the position. In case ...understand enough to relate it to the position I was applying for. After all I was only going to be interviewed... -
Mentoring for Scientists: I Have a Mentor, What Now?
TypeBlog PostPublishedMarch 6, 2014, 7:12 p.m....your meetings productive: Discuss logistics if appropriate: How often will you meet? How does the Mentor...the process of working on them is where growth happens. Mentors bring more than their knowledge and teaching... get a bunch of text messages asking me, “What happened? Where are you?” My gym posse holds me accountable... -
FlipGFP, a novel fluorescence protease reporter to study apoptosis
TypeBlog PostPublishedMay 21, 2019, 12:10 p.m.... the self-assembling capability of sfCherry approximately 20 fold by directed evolution. They then optimized...concept that the designed used in FlipGFP can be applied to other fluorescent proteins to create an array...and high brightness. This reporter achieves approximately 100-fold increase in fluorescence after cleavage... -
Addgene Depositors Get More Citations
TypeBlog PostPublishedApril 20, 2017, 2:30 p.m.... 3 Applied and Environmental Microbiology 1.6% 1.2% 0.75 2.3 4 2010 167 Applied and Environmental...Genetics & Heredity, Cell Biology, Biotechnology & Applied Microbiology, Biochemistry & Molecular Biology,... -
Summer SciComm Series: A PhD in Science Communication
TypeBlog PostPublishedAug. 23, 2022, 1:15 p.m....bioanthropology. Studying human health science made me happy, but it wasn’t long before I started becoming disillusioned...drives better after a few drinks. A family member approached me for advice on her baby’s health but adamantly...abstract where the real medical conditions had been swapped with made up illnesses. They were then asked to... -
New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor
TypeBlog PostPublishedApril 7, 2022, 1:15 p.m....origins of the system and explore advances that have happened since our original 2018 blog post. The origins... second generation of iGluSnFR, the Looger lab swapped eGFP for sfGFP to create SF-iGluSnFR. This improved...about other Fluorescent Biosensors A Practical Approach to Choosing the B(right)est Fluorescent Protein... -
Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond
TypeBlog PostPublishedJune 1, 2021, 1:15 p.m....perform the experiments to demonstrate that this approach was useful in humans. Then, one of them heard ...has been the realization that there is a great untapped potential to make science more efficient, open...activity is non-profit and pro bono. If this sounds appealing, join us and help make science a better place.... -
K. phaffii: Rising to the Occasion
TypeBlog PostPublishedApril 4, 2023, 1 p.m....used Parkinson’s experimental model. In fact, approximately 25% of yeast genes have a human counterpart,...2017). Beyond human relevance, yeast have many applications in food science and biotech due to its fermentation... ancient yeast strains and metazoan cells. Applications for K. phaffii There’s a number of different...