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We narrowed to 393 results for: ara-2

Showing: 381 - 393 of 393 results

  1. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...can still record the signal separately for each target.   Figure 2: Examples of simultaneous multiplex...or formalin-fixed, paraffin-embedded tissue sections, methanol- or paraformaldehyde-fixed cell samples...samples, or other preparations. Sequential IF For a sequential IF experiment (Figure 1A), you’ll block, add...different color fluorophore that you can image separately on your microscope. Abcam's sample protocol is...different antibodies, blocking, washing, and sample preparation steps, in order to ensure your antibodies are...Ilangovan, V., Lo, H., Olson, M., Mohamed, S. Y., Sarabipour, S., Varma, A., Walavalkar, K., Wissink, E. M...

  2. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    Published
    Jan. 4, 2022, 2:15 p.m.
    ...become a valuable asset yourself.     Fig 2: A diagram of the Thomas-Kilmann Conflict Model, courtesy...favorite books, Michael Ende’s Momo, the title character endears herself to the nearby village (and to ...or how completely different teams are tackling comparable conflicts. Is this level of domain knowledge ...

  3. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ...variety of environmental conditions, and double every 2 hours. Yeast are also the first eukaryotic genome ...as Parkinson’s disease (PD). PD is primarily characterized by protein misfolding that leads to a build-...23594743. PubMed Central PMCID: PMC3703927. Jennings, Barbara H. "Drosophila–a versatile model in biology & medicine...

  5. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ... Watch Joanne give her "Not" Networking 101 Talk 2. Tailor every talk to the occasion Don’t just plan...come up with clear and concise answers. This preparation will help you avoid giving only yes or no answers...

  6. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ...PubMed PMID: 3459152. PubMed Central PMCID: PMC323600. 2. Chester, Nicholas, and Daniel R. Marshak. "Dimethyl...small difference in size, the fragments should be separated on a high percentage agarose gel (~3%). Note that...

  7. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ... from damage (Abe & Lim, 2024).    Figure 2: A) When proteins (blue) interact with the air-water...RvLEAMshort (1:6 molar ratio) with 10 minutes of glutaraldehyde crosslinking: representative micrograph (C)...allowed the authors to determine structures at comparable or higher resolution than using more complex ...buffer additive MgCl2 and the crosslinking agent glutaraldehyde. Adding RvLEAMshort to the sample before plunge...

  8. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...biology 14.6 (2004): 757-764. PubMed PMID: 15582400. 2. Tomkinson, Alan E., et al. "DNA ligases: structure...the ligation junction were represented. Sixteen separate pools were prepared, each with a different complement...applications such as routine cloning or DNA library preparation. However, when a method relies on accurate ligation... 19329793. PubMed Central PMCID: PMC2719376. 5. Barany, Francis. "Genetic disease detection and DNA amplification...PMID: 1986365. PubMed Central PMCID: PMC50775. 6. Barany, Francis, and David H. Gelfand. "Cloning, overexpression...PMCID: PMC208194. 8. Luo, Jianying, and Francis Barany. "Identification of essential residues in Thermus...76.2 (1989): 245-254. PubMed PMID: 2753355. 12. Barany, Francis. "The ligase chain reaction in a PCR world...

  9. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ...experience in cancer research After graduating with a 2:1 BSc in Molecular Biology (roughly a B average in...really passionate about during my degree. I loved parasitology. However, when I enquired about roles in this...end of my three months, I fully understood why parasitology roles in the field require experience. Working...beginning of 2010, I explored options for going into parasitology but another major factor held me back; I do ...

  10. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...Nutr, 129 (2009a): 477–484. PubMed PMID: 10064313. 2. Chapman-Smith, A., & Cronan, J. E. “The enzymatic...Forster, A. C., Skingle, D. C., & Symons, R. H. “Preparation and uses of photobiotin.” Methods in Enzymology...

  11. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...effective recovery of knock-ins, but did result in 1-2 kilobases of additional sequence being introduced....brilliantly and irreverently illustrated in the parable of the Geneticist vs the Biochemist, we geneticists...selection allowed me to optimize a range of editing parameters: oligo repair templates with homology arms of... template design The Meyer lab meticulously characterized several aspects of editing. First, they devised...doi.org/10.17912/DDVH-BG64 Tzur YB, Friedland AE, Nadarajan S, Church GM, Calarco JA, Colaiácovo MP (2013)...

  12. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...Single-chain variable fragment (ScFv) Figure 2: Comparison between the IgG antibody and scFv. ...the surface of extracellular pathogens such as parasites or microbes, or to proteins expressed on the surface...containing a mixture of proteins. The proteins are separated based on molecular weight by SDS-PAGE, and are...

  13. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ...ask new and interesting questions in your own lab. 2. Seek multiple mentors during your postdoc At the ...corresponding author publications (published, not in preparation) A history of successful funding (fellowship ...
Showing: 381 - 393 of 393 results